What are the limitations of negative, spore, and capsule staining?
What alternative or supplementary methods can be used to account
for those limitations
Place references and credible sources.

Answers

Answer 1

Negative, spore, and capsule staining are three common methods of bacterial staining used to differentiate bacterial species. However, these staining methods do have certain limitations.

Negative staining can only differentiate bacteria that have a cell wall, while spore and capsule staining may not be effective on bacteria that don't produce spores or capsules, respectively. In addition, spore and capsule staining may produce false positives.

Alternative or supplementary methods that can be used to account for these limitations include Fluorescence In Situ Hybridization (FISH), Polymerase Chain Reaction (PCR), and Molecular Typing techniques such as Ribotyping, Amplified Fragment Length Polymorphism (AFLP), or Repetitive Element PCR (REP-PCR).

References:
Fernandez-Cuenca, F. et al. (2014). Molecular typing for bacterial epidemiology. FEMS Microbiol Rev 38(4): 768–800. doi:10.1111/1574-6976.12090
Aydin, A., Sengul, M. (2016). Bacterial Staining Techniques. In: Clinical Microbiology Procedures Handbook 4th Edition. ASM Press.
Holt, J.G. et al. (1994). Bergey’s Manual of Determinative Bacteriology, 9th Edition. Williams & Wilkins, Baltimore, MD.

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Related Questions

Patient presents with airway anatomy that makes visualization of the airway structures difficult during direct laryngoscopy. This general term is known as________

Answers

The general term for airway anatomy that makes visualization of the airway structures difficult during direct laryngoscopy is known as difficult airway.

This can be caused by various factors such as obesity, short neck, small mouth opening, or limited neck mobility. It is important to identify a difficult airway prior to the procedure in order to take necessary precautions and ensure patient safety.

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The 2010 Deepwater Horizon oil spill in the Gulf of Mexico released millions of gallons of oil into the ocean. This was a major human-caused ecosystem disturbance in the area. Which statements BEST describe what happened after the spill?

Answers

None of the above statements accurately describe what happened after the Deepwater Horizon oil spill.

What is the eco system?

An ecosystem is a dynamic complex of plant, animal, and microorganism communities and the non-living environment, interacting as a functional unit. It can be a river or a coral reef, a desert or a forest, around us or far away. All parts of an ecosystem, including the air, water, soil, sunlight, plants, animals, and microorganisms, are connected and rely on each other to survive. Humans are also a part of many ecosystems and have an important role to play. Ecosystems provide a variety of services, such as food, medicine, and clean air, water, and soil.

In fact, the oil spill had a devastating effect on the marine life in the Gulf of Mexico. The spill caused a significant decrease in the diversity of species in the Gulf of Mexico, causing a massive die-off of fish, birds, mammals, and other wildlife. The oil also caused a major disruption to the food web in the Gulf, making it difficult for some species to find food and survive. The spill also caused long-term damage to the Gulf's ecosystem, with some species still feeling the effects of the spill even a decade later.

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Complete questions as follows-
The 2010 Deepwater Horizon oil spill in the Gulf of Mexico released millions of gallons of oil into the ocean. This was a major human-caused ecosystem disturbance in the area. Which statements BEST describe what happened after the spill?

1. The oil spill caused a major decrease in the diversity of species in the Gulf of Mexico.
2. The oil spill caused a major increase in the number of fish in the Gulf of Mexico.
3. The oil spill caused an increase in the number of birds and mammals in the Gulf of Mexico.

In three short sentences, explain the application of Hardy-Weinberg Equilibrium to detect the presence or absence of evolution: - Sentence 1 - Define the Hardy-Weinberg equilibrium in terms of allele

Answers

The Hardy-Weinberg equilibrium is a condition that describes the genetic composition of a population that is not evolving. In other words, the Hardy-Weinberg principle states that under certain conditions, the frequencies of alleles and genotypes in a population will remain constant over generations.

This principle is important in evolutionary biology because it serves as a null model for studying how populations change over time. In the Hardy-Weinberg equilibrium, the frequencies of alleles and genotypes in a population remain constant over generations. In order for a population to be in Hardy-Weinberg equilibrium, several conditions must be met.

First, the population must be large and randomly mating.

Second, there can be no mutations, no gene flow, no natural selection, and no genetic drift. If these conditions are met, then the frequencies of alleles and genotypes in the population will remain constant over time.

The Hardy-Weinberg equilibrium is useful in detecting the presence or absence of evolution because it allows researchers to compare the actual frequencies of alleles and genotypes in a population to the expected frequencies under the Hardy-Weinberg principle. If the actual frequencies differ significantly from the expected frequencies, then it is likely that the population is evolving.

For example, if the frequency of a particular allele increases over time, then it is likely that this allele is undergoing positive selection and is becoming more common in the population. Conversely, if the frequency of a particular allele decreases over time, then it is likely that this allele is undergoing negative selection and is becoming less common in the population.

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How is it possible that our 25,000 genes are able to produce the
hundreds of thousands of proteins made by the body?

Answers

It is possible that our 25,000 genes are able to produce the hundreds of thousands of proteins made by the body through a process called alternative splicing.

Alternative splicing is defined as a process by which a single gene can produce multiple proteins. During alternative splicing, different parts of the gene are spliced together in different ways to produce different mRNA transcripts. These different mRNA transcripts can then be translated into different proteins. This allows a single gene to produce multiple proteins, allowing our 25,000 genes to produce the hundreds of thousands of proteins made by the body. A protein-coding DNA transcription unit may comprise both a coding sequence that will be translated into the protein and regulatory sequences that will control and regulate the synthesis of that protein.

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Trace a troponin molecule from the great cardiac vein of the
heart to an extensor muscle on the dorsum of the foot.

Answers

The troponin molecule begins its journey in the great cardiac vein of the heart. From here, it enters the systemic circulation and is transported through the aorta and down the femoral artery. It then enters the iliac artery and proceeds to the dorsalis pedis artery. From here, it is transported to the dorsum of the foot, where it enters the extensor muscle.


A troponin molecule is a protein that is involved in muscle contraction. It is found in both skeletal and cardiac muscle cells. Here is the step-by-step process of how a troponin molecule travels from the great cardiac vein of the heart to an extensor muscle on the dorsum of the foot:

1. The troponin molecule is first found in the cardiac muscle cells of the heart.
2. It then enters the bloodstream through the great cardiac vein.
3. The troponin molecule travels through the circulatory system until it reaches the lower extremities.
4. It then enters the skeletal muscle cells of the extensor muscles on the dorsum of the foot.
5. Once inside the muscle cell, the troponin molecule binds to actin and tropomyosin, which are other proteins involved in muscle contraction.
6. This binding causes the muscle to contract, allowing the extensor muscles on the dorsum of the foot to move.

In summary, a troponin molecule travels from the great cardiac vein of the heart, through the circulatory system, and into the extensor muscles on the dorsum of the foot, where it plays a role in muscle contraction.

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In the fruit fly Drosophila melanogaster, the alleles for sparkling eyes and shaven bristles are so tightly linked that essentially no crossovers occur between these two loci. A dihybrid with this genotype,
spa +
sv/spasv +
, is test crossed to the tester spa sv / spa sv. What percentage of progeny would you expect to have sparkling eyes and shaven bristles? a) 0%
b)5%
c)25%
d)35%
E)40%

Answers

If the alleles for sparkling eyes (spa) and shaven bristles (sv) are so tightly linked that essentially no recombination occur between these two loci i.e. there will be a) 0% recombination.

They will behave as if they are on the same chromosome, and will segregate together during meiosis. Therefore, the expected gametes produced by the dihybrid parent with genotype spa + sv/spa sv + are:

spa + sv

spa + sv +

spa sv

spa sv +

The tester parent spa sv / spa sv will only produce gametes with the spa sv genotype.

When genes were clustered on the same chromosome, Morgan and his team discovered that certain genes were extremely tightly coupled (exhibited very little recombination), while others were lossely linked (showed higher recombination).

The chance of recombination is 50% when the genes are far from one another or on different chromosomes. In this instance, the two loci's allele inheritance is independent. Recombination frequency less than 50% indicates a connection between the two loci. And tightly linked genes show 0 % recombination.

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Enzymes increase the reaction rate by:
allowing reactions to proceed at a higher temperatures
increasing the energy of collisions
increasing the concentration of substrate
lowering the activation ener

Answers

Enzymes increase the reaction rate by lowering the activation energy. Thus, Option D is correct.

This allows the reaction to proceed more quickly and efficiently, as less energy is required to initiate the reaction.

The other options listed, such as allowing reactions to proceed at a higher temperature, increasing the energy of collisions, and increasing the concentration of substrate, are not accurate descriptions of how enzymes increase reaction rates. Enzymes function by lowering the activation energy, which is the minimum amount of energy required for a reaction to occur.

By lowering this energy barrier, enzymes allow reactions to proceed more quickly and efficiently.

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i need help w number 17

Answers

Answer:

photosynthesis

Explanation:

What enzyme (or chemical method) was used on Protein Example #2 to make the D fragments?
a. trypsin
b. chymotrypsin
c. V8 protease
d. asp-N-protease
e. pepsin
f. cyanogen bromide
"D" Fragments – Protein #2
D-1) E A
D-2) V K
D-3) M L E G K
D-4) W F N S E K
D-5) G P Q A A N V T K
D-6) T L E E G Q A V S F E I V E G N R
D-7) G F G F I E V E G Q D D V F V H F S A I Q G E G F K

Answers

The enzyme used on Protein Example #2 to make the D fragments is trypsin.

Trypsin is a protease, meaning it breaks down proteins, and it specifically cleaves proteins at the carboxyl side of lysine and arginine. This means that trypsin cleaves after the amino acids lysine (K) and arginine (R).

When trypsin is used, the resulting cleavage sites produce a series of peptides, which are referred to as the "D" fragments. The "D" fragments of Protein #2 are as follows: D-1) E A, D-2) V K, D-3) M L E G K, D-4) W F N S E K, D-5) G P Q A A N V T K, D-6) T L E E G Q A V S F E I V E G N R, D-7) G F G F I E V E G Q D D V F V H F S A I Q G E G F K.

Therefore, we can say that trypsin is used on Protein Example #2 to make D fragments.

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in the center region of the retina and has a high density of smaller, tightly-packed cones with high acuity. is called?

Answers

The center region of the retina that has a high density of smaller, tightly-packed cones with high acuity is called the fovea.

It is responsible for sharp central vision, which is necessary for activities like reading, driving, and recognizing faces. The fovea is located in the macula, which is the central part of the retina. Cones are the photoreceptor cells that are responsible for color vision and are most concentrated in the fovea. This is why the fovea is important for tasks that require detailed vision and color perception. The retina is a complex structure that includes several layers of neurons and supporting cells, as well as blood vessels and other structures that nourish and protect the delicate cells within it.

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1a) The 3 carbon molecule generated during glycolysis is
called _____________________.
A. struvate
B. acetyl-CoA
C. oxaloacetate
D. pyruvate.

Answers

Option d) is the correct answer. Pyruvate is a 3-carbon molecule generated during glycolysis. It is the end-product of the preparatory reaction of glycolysis, which is the breakdown of glucose into two molecules of pyruvate. Pyruvate is then used in a variety of metabolic processes, such as energy production, biosynthesis, and the synthesis of other molecules.

In energy production, pyruvate is converted into ATP, the energy currency of the cell. This is done through a process called oxidative phosphorylation, in which pyruvate is oxidized to form acetyl-CoA, which is then further oxidized and combined with ADP to form ATP.

In biosynthesis, pyruvate is used to make other molecules, such as amino acids, lipids, and nucleic acids. It can be converted into various intermediates such as oxaloacetate, malate, and fumarate, which can then be used to make other molecules.

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Question #10: Let's say the 1:10 dilution of ONP solution has an
absorbance value of 1.026. The linear regression equation is
y=0.0014x + 0. What is the amount of ONP (µmol) in
10 mL of ONP solution?

Answers

The amount of ONP in 10 mL of the 1:10 dilution of ONP solution is 733.14 µmol.

The question is asking what the amount of ONP (µmol) is in 10 mL of the 1:10 dilution of ONP solution given that its absorbance value is 1.026 and the linear regression equation is y=0.0014x + 0.
To solve this problem, we need to use the linear regression equation and the absorbance value. The equation can be rearranged to solve for x (the amount of ONP):
x = (y - 0) / 0.0014
We can substitute the absorbance value for y and get:
x = (1.026 - 0) / 0.0014
x = 733.14 µmol

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Genetic experiments often require constructing specific chromosomes with the desired genotype, which involves matings to produce specific recombinant progeny. The multiply-marked X chromosome we will use in lab 7 carries four markers: yellow (y). crossveinless (ev), vermillion (w and forked O. However, as vermillion is not used in the lab. Dr. Gilliland wants to build a tester stock that only carries mutants for y, ev and f.
1a) This goal is easier because these are X-linked genes. What feature of X-linked genes helps simplify finding the right recombinant chromosome? (2 pts)
1b) BRIEFLY Describe a hang seleme has could produce that desired chromosome. You have two homozygous parent stocks to start with: the current multiple-mutant stock, y- cv- y- f-, and the wildtype strain Oregon-R, which is y+ ev+ v+ f+. Indicate the males and females you would cross each generation, and state what outcome(s) would be needed to create the desired chromosome. (Hint: the shortest possible solution requires 2 generations, but other crosses work too.) (8 pts)
parents: y- cv- y- f- y+ ev+ v+ f+
goal: y- cv- v+ f-

Answers

The feature of X-linked genes that helps simplify finding the right recombinant chromosome is that they are passed directly from father to daughter. This means that any desired recombinant chromosome can be obtained in one generation of mating.

To create the desired chromosome, a two-generation cross could be used. In the first generation, the wild-type strain Oregon-R (y+ ev+ v+ f+) should be crossed with the current multiple-mutant stock (y- cv- y- f-).

The resulting F1 progeny should be crossed back to the multiple-mutant stock (y- cv- y- f-) in the second generation. In the F2 progeny, the desired chromosome (y- cv- v+ f-) should be present, as it is the combination of the y- cv- from the multiple-mutant stock, and the v+ f- from the wild-type strain Oregon-R.

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"Which one of the following is the cause of malaria? a. Parasitic worm b. A toxic chemical in mosquito saliva c. Severe immune reaction to mosquito bite d. Single-celled protist

Answers

The cause of malaria is a single-celled protist, specifically the a) Plasmodium parasite.

Malaria is a serious and sometimes fatal disease that is spread by the Anopheles mosquito when it bites an infected human. The parasite enters the bloodstream and travels to the liver, where it multiplies and then re-enters the bloodstream, infecting red blood cells.

This causes flu-like symptoms, including fever, chills, and muscle aches. If left untreated, malaria can cause severe complications such as anemia, organ failure, and even death.

While there are effective treatments for malaria, prevention through mosquito control measures such as insecticide-treated bed nets and indoor residual spraying is also critical to reducing the spread of this disease.

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The plasma membrane is made of phospholipids. A phospholipid molecule has a polar head and two non-polar tails. How does the plasma membrane work? O Polar molecules can interact with the polar heads and cross the membrane. Polar molecules form covalent bonds with the polar heads and become permanently attached to them. Polar molecules are excluded from the interior of the membrane by repulsion from non-polar tails. O Non-polar molecules are excluded from the interior of the membrane by repulsion from non-polar tails. O Non-polar molecules are attracted to the non-polar tails, thus not being able to cross the membrane.

Answers

The statement that describes how plasma membrane work is as follows: Polar molecules form covalent bonds with the polar heads and become permanently attached to them (option B).

What is plasma membrane?

Plasma membrane is the semipermeable membrane that surrounds the cytoplasm of a cell. The semipermeability denotes that it allows some substances to pass and doesn't allow others.

According to this question, the plasma membrane is made of phospholipids. A phospholipid molecule has a polar head and two non-polar tails.

Gases, hydrophobic molecules, and small polar uncharged molecules can diffuse through phospholipid bilayers. Larger polar molecules and charged molecules cannot.

Since the heads are hydrophilic, they face outward and are attracted to the intracellular and extracellular fluid.

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how many genetically different children could your parents have produced with their combinations of gametes
- 64 trillion
- 46
- 8 million
- 285

Answers

The answer is Option C: 8 million. 8 million genetically different children could your parents have produced with their combinations of gametes.

Each parent produces 23 pairs of chromosomes in their gametes, which combine to form a zygote with 46 chromosomes. Because each chromosome pair can combine in 2 different ways, there are 2^23 possible combinations for each parent, which equals 8,388,608 or approximately 8 million different possible combinations of gametes.

Therefore, your parents could have potentially produced 8 million genetically different children.

Humans have 23 pairs of chromosomes. That means that one person could produce 223 different gametes. In addition, when you calculate the possible combinations that emerge from the pairing of an egg and a sperm, the result is (223)2 possible combinations.Thus the correct option is C : 8 million.

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Why do you think that most animals have some variant of either a nerve net or a central nervous system? Why is there not just a single type of nervous system? Why not more than 2 main types of nervous systems?

Answers

Most animals have some variant of either a nerve net or a central nervous system because these two systems have evolved to be the most effective and efficient ways of transmitting and processing information throughout the body.

A nerve net, which is found in simpler animals like jellyfish and anemones, is a diffuse network of neurons that allows for simple responses to stimuli. A central nervous system, which is found in more complex animals like mammals and birds, is a more centralized system that allows for more complex behaviors and responses to stimuli. There is not just a single type of nervous system because different animals have different needs and environments. A nerve net is sufficient for simple animals that do not need to process complex information, while a central nervous system is necessary for more complex animals that need to process more information and make more complex decisions.

There are not more than 2 main types of nervous systems because the nerve net and central nervous system have been shown to be the most effective and efficient systems for transmitting and processing information. Other types of nervous systems may have evolved in the past, but they were not as successful and therefore did not become the dominant systems.

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The similarities in the mitochondrial DNA and some proteobacteria indicate:
A. that there was a proteobacteria ancestor of eukaryotic cells.
B. proteobacteria and mitochondria are similar as a result of convergent evolution.
C. that mitochondria expelled from eukaryotic cells evolved into proteobacteria.
D. that mitochondria are likely the result of endosymbiosis of a proteobacteria and a eukaryotic cell.

Answers

The similarities in the mitochondrial DNA and some proteobacteria indicate that mitochondria are likely the result of endosymbiosis of a proteobacteria and a eukaryotic cell. The correct option is D.

Mitochondria contain their own DNA which is similar to that of some proteobacteria, and is different from the DNA of the cell in which it resides.

This suggests that the mitochondria originated from an independent organism that became incorporated into a eukaryotic cell and ultimately evolved into what it is today. This is known as the endosymbiosis theory.  
1. Mitochondria contain their own DNA which is similar to some proteobacteria.
2. This suggests that the mitochondria originated from an independent organism.
3. This organism became incorporated into a eukaryotic cell.
4. This is known as the endosymbiosis theory and indicates that mitochondria are likely the result of endosymbiosis of a proteobacteria and a eukaryotic cell.

Hence, the correct option is D.

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Describe at least 3 aspects of the scientific process or writing that you feel confident about that perhaps you didn’t before this course. A good response might be: "I feel more confident in my ability to perform a literature search using appropriate keywords, and to locate pertinent articles."

Answers

I feel more confident in my ability to perform a literature search using appropriate keywords, and to locate pertinent articles.

I have also become better at formulating a hypothesis based on existing research and critically analyzing scientific data.

Lastly, I have become more confident in my ability to write scientific reports with clear and concise language that is supported by facts and evidence.

In this course, I have gained a better understanding of the scientific process and how to apply it to my writing.

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prevention of aging and cosmetic rejuvenation are two potential
applications of _____ research.

Answers

Prevention of aging and cosmetic rejuvenation are two potential applications of stem cell research.

Stem cells аre а type of cell thаt cаn divide indefinitely аnd differentiаte into а wide vаriety of cell types. This mаkes them incredibly vаluаble for medicаl reseаrch, аs they cаn be used to study аnd potentiаlly treаt а wide vаriety of diseаses аnd conditions. One potentiаl аpplicаtion of stem cell reseаrch is the prevention of аging.

By studying how stem cells аge аnd whаt fаctors contribute to this process, reseаrchers mаy be аble to develop treаtments thаt cаn slow or even reverse the аging process. Аnother potentiаl аpplicаtion of stem cell reseаrch is cosmetic rejuvenаtion. By using stem cells to regenerаte dаmаged or аging skin, reseаrchers mаy be аble to develop treаtments thаt cаn improve the аppeаrаnce of skin аnd reduce the signs of аging.

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What
would you expect a down mutation in the
-35
labeled DNA would do to the binding of the o factor to region 4.2
in this assay (panel b, red curve) ?

Answers

A down mutation in the -35 labeled DNA region that binding of the o factor to region 4.2 in the assay (panel b, red curve) would lead to a reduced binding of the o factor

DNA is a self-replicating, double-stranded, helical molecule that is responsible for passing genetic information from one generation to the next. It is made up of nucleotides that are linked together by covalent bonds. A mutation is a change in the DNA sequence that alters the structure or function of the encoded protein. There are two primary types of mutations: (i) Point mutations and (ii) Frame-shift mutations.

In point mutations, one nucleotide is changed to another, whereas in frame-shift mutations, one or more nucleotides are added or deleted from the sequence, resulting in a change in the reading frame.Both types of mutations can cause a reduction or complete loss of protein function. The effect of a down mutation in the -35 labeled DNA region on the binding of the o factor to region 4.2 in the assay (panel b, red curve) would be to decrease the binding of the o factor to region 4.2.

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Woodpeckers directly create holes in wood, providing homes for other species (like chickadees which always nest in cavities). These habitat alterations are vital: ~90 percent of all available nesting cavities are created by woodpeckers. A species that significantly and directly impacts habitat like this is called a/an…?

Answers

A species that significantly and directly impacts habitat like woodpeckers do is called a keystone species.

Keystone species play a crucial role in maintaining the structure and function of an ecosystem. They have a disproportionately large effect on the habitat and other species within it, and their removal can lead to drastic changes in the ecosystem.

In the case of woodpeckers, they create nesting cavities for other species, which is vital for the survival of those species.

Without woodpeckers, there would be a significant decrease in available nesting cavities, and this could negatively impact the population of species that rely on them for nesting.

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After Counting Platelets On Both Sides Of The Counting Chamber, The Technician Got 210 And 235 Respectively. If 20ul Of Blood Was Added To 1.98ml Of The Diluent, Calculate In One (1) Litre The Number Of Platelet In The Blood If The Volume Of The Chamber Is 0.02ml. Comment On The Results.

Answers

The number of platelets in one (1) litre of the blood is [tex]1.1125[/tex] × [tex]10^{9}[/tex] platelets / litre.

To calculate the number of platelets in one (1) litre of blood, we need to first find the average number of platelets counted in the counting chamber. This can be done by adding the two counts and dividing by 2:

Average platelets = (210 + 235) / 2 = 222.5

Next, we need to find the dilution factor, which is the ratio of the volume of blood to the total volume of the solution:

Dilution factor = volume of blood / total volume

= 20ul / (20ul + 1.98ml)

= 20ul / 2000ul

= 0.01

Now, we can use the average platelets, dilution factor, and volume of the counting chamber to calculate the number of platelets in one (1) litre of blood:

Platelets in 1 litre = (average platelets / dilution factor) * (1000ml / volume of counting chamber)

= (222.5 / 0.01) * (1000ml / 0.02ml)

= [tex]1.1125[/tex] × [tex]10^{9}[/tex] platelets / litre

As for the comment on the results, it would depend on the normal range of platelet count for the individual or population being tested. Generally, a normal platelet count ranges from 150,000 to 450,000 platelets per microliter of blood. In this case, the calculated platelet count of [tex]1.1125[/tex] x [tex]10^{9}[/tex] platelets / litre (or 1,112,500 platelets / microliter) is within the normal range.

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Which of these solutions has the lowest concentration of H*? O
coffee, pH 5 O stomach acid, pH 2 bleach, pH 13

Answers

Bleach is the solution with the lowest concentration of hydrogen ions (H*) with a pH of 13. This is because pH measures the concentration of H* in a solution, and the lower the pH value, the higher the concentration of H*.

The solution with the lowest concentration of H* is bleach, with a pH of 13. The pH scale measures the concentration of hydrogen ions (H*) in a solution. The lower the pH value, the higher the concentration of H*. Therefore, a solution with a pH of 2, like stomach acid, has a higher concentration of H* than a solution with a pH of 5, like coffee. Bleach, with a pH of 13, has the lowest concentration of H* of the three solutions listed.

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Explain molecularly and with genotypes what happened to cause Stern’s (1936) observation of twin spots in fruit flies (one patch with recessive bristle expression and one adjacent patch with recessive body color expression in a fly heterozygous for both linked traits):

Answers

Stern's 1936 observation of twin spots in fruit flies can be explained molecularly and with genotypes.


The genetic mechanism underlying the twin-spot phenotype is described below.

Molecularly: Stern observed a pair of phenotypically distinct spots on the wings of a heterozygous fly. It was hypothesized that this phenotype was caused by somatic loss of heterozygosity, in which the two genetically distinct cells that make up the wing were differentially affected by mitotic recombination, resulting in the two-color spots.Genotypes: This phenotype is caused by genetic recombination between the recessive bristle and body color genes, both of which are located on the X chromosome. The phenomenon of genetic recombination between linked loci is referred to as crossing over, and it is critical for producing genetic diversity. In a heterozygous fly, crossing over between the homologous chromosomes during meiosis results in the production of recombinant gametes, which have new combinations of alleles from both parents, thus causing twin spots on the wings of the fly.

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13. The brain signals the body of an arimal to move by transmittieg electrical impulses through the neurons. An crimal is then able to walk or run because

Answers

By sending electrical impulses through neurons, the brain signals the body to move, activating the muscles that are responsible for movement.

The electrical impulses that travel between brain neurons are referred to as brain signals. For the brain to regulate and synchronise the body's movements, thoughts, and emotions, these messages are necessary. There are billions of neurons in the brain, and they all communicate with one another via a sophisticated network of synapses. A neuron sends off an electrical impulse that travels down its axon and causes the release of neurotransmitters when it receives a signal from another neuron. A new electrical impulse is then started when these neurotransmitters bind to receptors on the surface of additional neurons. The brain is able to regulate all facets of the body's behaviour and function, from simple physical movements to intricate cognitive functions, by sending and receiving these signals.

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Compare and contrast the sporophyte generation of mosses and
ferns. Your reply could take into consideration vascular tissue,
dominant form, diploid or haploid, and what happens to it as the
life cycle ?

Answers

The sporophyte generation of mosses and ferns is the stage in the life cycle of these plants where a diploid multicellular organism is formed, usually from a haploid spore. The dominant form of the sporophyte in both mosses and ferns is a stalked structure that grows from the ground.

In mosses, the sporophyte has no vascular tissue, meaning it is unable to transport water and other nutrients effectively. Ferns, however, do have vascular tissue in the sporophyte stage, which aids in transporting water and other nutrients more efficiently.

Additionally, the sporophyte of ferns is the dominant form, while the sporophyte of mosses is less well developed.

Finally, the sporophyte of mosses and ferns will eventually produce spores, haploid cells, which will then develop into gametophytes, which in turn produce gametes. These gametes will then fuse and form a zygote, which will develop into a sporophyte.

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What are the serum markers at diagnosis in testicular tumors and
which are important in which tumors? Explain in details.

Answers

The main serum markers that are commonly used in the diagnosis of testicular tumors are alpha-fetoprotein (AFP), beta-human chorionic gonadotropin (beta-hCG), and lactate dehydrogenase (LDH).

Serum markers are substances that can be detected in the blood and can be used to help diagnose and monitor testicular tumors.

Alpha-fetoprotein (AFP) is a protein that is normally produced by the liver and yolk sac during fetal development. Elevated levels of AFP can be found in patients with non-seminomatous germ cell tumors, such as embryonal carcinoma, yolk sac tumor, and choriocarcinoma.

Beta-human chorionic gonadotropin (beta-hCG) is a hormone that is normally produced by the placenta during pregnancy. Elevated levels of beta-hCG can be found in patients with choriocarcinoma and some cases of embryonal carcinoma.

Lactate dehydrogenase (LDH) is an enzyme that is found in many tissues throughout the body. Elevated levels of LDH can be found in patients with both seminomatous and non-seminomatous germ cell tumors.

It is important to note that not all testicular tumors will produce elevated levels of these serum markers. Seminomas, for example, typically do not produce elevated levels of AFP or beta-hCG. Therefore, the presence or absence of these serum markers can help to differentiate between different types of testicular tumors and can be used to guide treatment decisions.

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What would happen to transcription at the GAL1 gene in yeast if
the Gcn5 subunit of SAGA was deleted? Why? (Assume cells growing in
galactose.)

Answers

If the Gcn5 subunit of SAGA was deleted, transcription at the GAL1 gene in yeast would be reduced. This is because Gcn5 is an important component of the SAGA complex, which is involved in the activation of transcription at the GAL1 gene.

Without Gcn5, the SAGA complex would not be able to properly recruit RNA polymerase II to the GAL1 promoter, leading to reduced transcription of the gene.

Additionally, Gcn5 is responsible for the acetylation of histones, which is important for the accessibility of the DNA to the transcription machinery.

Without Gcn5, the histones would not be properly acetylated, leading to a more closed chromatin structure and reduced transcription at the GAL1 gene.

Therefore, deletion of the Gcn5 subunit would have a negative impact on transcription at the GAL1 gene in yeast cells growing in galactose.

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Which statement is the best description of science?

Answers

Answer:

the systematic study of the structure and behavior of the physical and natural world through observation, experimentation, and the testing of theories against the evidence obtained

Explanation:

Explanation:

Science is the pursuit and application of knowledge and understanding of the natural and social world following a systematic methodology based on evidence.

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