How can you guarantee a high expression of your protein in any expression vector?

Answers

Answer 1

Answer:

protein expression refers to the way in which protein are synthesized, modified and regulated in living organisms.

Explanation:

In the protein research,the term can apply either the object of study or the laboratory techniques required to manufacture proteins.

How Can You Guarantee A High Expression Of Your Protein In Any Expression Vector?

Related Questions

ions but you do not want to increase the volume of the blood by reabsorbing large amounts of water from the filtrate. Assuming the aldosterone and ADH are both present, how would you adjust the hormones to accomplish the task

Answers

Answer:

Aldosterone and ADH are part of the renin angiotensin aldosterone system, this system is a system regulated by the kidney and lungs to be able to raise blood pressure.

Explanation:

This system is a retainer of liquids and water, to increase the blood volume of our body, in this way arterial hypotension is compensated.

Another mechanism with which blood volume can be increased is with the stimulation of thirst, and the need to ingest a liquid because of the dry mouth that our central nervous system perceives.

Both mechanisms prevent hypotension and dehydration.

FIGURE 20.15 Brain structure

Answers

The brain is structured in 3 parts: rombencephalon, midbrain and forebrain.

The robencephalon is a little evolved structure and therefore has a very simple and low complexity organization. It is divided into three parts: the medulla oblongata, the annular protuberance and the cerebellum. The robencephalon is responsible for functions that are basic and essential for our organism, in addition, that part of the brain is responsible for our movement. Despite being responsible for basic functions, injuries in any part of the brain can cause serious problems to the body.

The midbrain is responsible for conducting the motor and sensory impulses essential for the full functioning of our body. Its functioning is very important for the full functioning of the organism, without the midbrain, or with the impaired midbrain, the body may present abnormal and strange movements such as inflexibility, spasms and tremors,

The forebrain is the most evolved, developed and complex structure of the brain. It consists of the diencephalon, thalamus, hypothalamus and cerebrum. Due to its complexity, it is responsible for the most complex functions of the organism, being highly efficient and causing serious damage if it does not work properly.

active transport occurs in cells for example when Na-k pump is at work.Any process that involves active transport most often involves the​

Answers

Answer:

Any process that involves active transport most often involves the​ expenditure of energy in the form of ATP hydrolysis.

Explanation:

Active transport in cells is a form of transport which involves the transport of solute molecules across a membrane against a concentration gradient using energy provided from some chemical reaction occuring in the cell.

Active transport is an endergonic (energy-requiring) process and therefore, must proceed only when coupled to an exergonic (energy-releasing) process such as the breakdown of ATP, an oxidation reaction, absorption of sunlight, etc.

In many instances in cell, such as the Na/K pump, ATP hydrolysis is the the common exergonic reaction to which active transport is coupled to.

History has demonstrated that the techniques employed in this laboratory exercise often overestimate an individual's actual resting tidal volume. List some reasons why these laboratory techniques might produce abnormally high values for resting tidal volume.

Answers

Answer:

See below

Explanation:

One possible reason here could be the fact that since the person is being watched while doing this exercise, they might tend to breather deeper than they normally do. Secondly, it may also be because the person is trying to make up for the spit filter and flow meter by breathing harder/sharper than they normally would.

Why would satellite imagery be more useful than a map in some instances?

Answers

Answer:

topography

Explanation:

Answer:

A.

Explanation:

took test

What can happen to freshwater animals if they do not have mechanisms that remove water?why does this happen??

Answers

Answer:

Freshwater animals have lower water and higher salt concentrations than the environment in their cells. This means water constantly tries to diffuse into the animals, which can bloat them. They have special cell processes that remove extra water.

Explanation: Penn Foster

Which of the following is the largest known gravitationally bound structure in the universe?
Which of the following is the largest known gravitationally bound structure in the universe?


Galaxy


Cluster


Solar system


Local Group

Answers

Answer:

Cluster

Explanation:

A cluster is the largest gravitationally bound object in the universe.

Answer:

Cluster

Explanation:

A cluster is the largest gravitationally bound object in the universe.

Label parts A and B in this picture of saprophytes (fungi)

Answers

Answer: A- Sporangium

              B- Rhizoids

Explanation:

What is one way to classify mud found in an ecosystem? organism population biotic factor abiotic factor

Answers

Answer:

abiotic factor

Explanation:

Mud, while hosting many multicellular and unicellular organisms, simply refers to the combination of water and dirt, neither of which are living.

Answer:

abiotic factor

Explanation:

Name the components that gets digested in stomach and pancreas.

Answers

Explanation:

example protein can be digested both in stomach and pancreas

Protein can be digested in the stomach and pancreas because both secretes proteases (enzymes to digest proteins). The stomach secretes pepsin which works optimally under acidic conditions. The pancreas secretes trypsin which works optimally in neutral conditions

The adaptive structure and function of leaves and stems helps the plant

A create its own energy
B prevent wilting
C produce haploid gametes
D produce diploid gametes

Answers

Answer:

I think, your answer is,. create it's own energy.

By the process of photosynthesis, where stems and leaves do their major function.

Explanation:

Hope it helps you........

Answer:

A.  Create it's own energy

Explanation:

Plants have adaptations that assist in the creation of energy such as: stems support and hold the leaves above the ground for more light, surface of leaves exposed to sunlight.

What substance are the bacteria (microorganisms) breaking down in the digestive system of cattle? Describe the digestion of this substance.

Answers

Answer:

The Bacteria or microorganisms are present in the rumen of cattle that aid in the digestion of cellulose and grains in the digestive system of cattle.

The process of digestion of cellulose by bacteria in cattle is anaerobic digestion and includes two steps – the production of the required enzymes and the fermentation.

In the fisrt step of production of enzyme, the microbes or bacteria are found in the first chamber of the stomach. These bacterias or microbes releases several enzymes that helps in splitting the cellulose into smaller carbs such as glucose.

These smaller carbs are the move to thrd chamber of the stomach and fermented. the required nutrients are absorbed. The digested food then finally reached at fourth chamber (acidic part), where the remained food is digested and passes to the small and large intestines.

whats 20x20? thank you

Answers

Answer:

400 yayyyyyy

Explanation:

yvhjghkkkkhhh

Answer:400

Explanation:

20

x20

_____

00

+400

--------

400

Approximately how much methane does one cow produce per year? How does the greenhouse gas warming effect of methane compare to carbon dioxide? Describe the organisms that digest and ferment the hay inside of the cow’s stomach? How are scientists reducing the amount of methane released from cows?

Answers

Answer:

Cattle and other ruminants are significant producers of the greenhouse gas methane—contributing 37 percent of the methane emissions resulting from human activity. A single cow on average produces between 70 and 120 kg of methane per year and, worldwide, there are about 1.5 billion cattle.

While carbon dioxide is typically painted as the bad boy of greenhouse gases, methane is roughly 30 times more potent as a heat-trapping gas. ... As temperatures rise, the relative increase of methane emissions will outpace that of carbon dioxide from these sources, the researchers report.

The cow's rumen is like a large fermentation vat. More than 200 different bacteria and 20 types of protozoa help the cow to utilize fibrous feedstuffs and non-protein nitrogen sources. ... Bacteria adhere to the feed and gradually digest the fermentable material.

“You can probably reduce methane by about 20-25% by altering diet,” he says. One study by researchers at the University of California, Davis, estimated it might be possible to reduce global methane emissions from cows by 15% by changing their diet.

Explanation:

Hope it helps.

Write the numbers in the correct order to sequence the food through the digestive tract. 1- Peristalsis begins in the esophagus. 2- Food is pushed into the throat with the tongue. 3- Epiglottis closes to allow food to pass to the esophagus. 4-Chyme mixes with digestive enzymes. 5- Esophageal sphincter allow food to pass to the stomach. 6- Food is mixed with digestive juices forming the chyme that moves to the small intestine. 7- Stool moves to the rectum then to the anus. 8- Digested nutrients move to the blood. 9- Water is absorbed and waste is transformed into the stool. 10- Waste product moves into the large intestine. )

Answers

Answer:

2: food is pushed into the throat with the tongue, 3 epiglottis closes to allow food to pass esophagus, 1 peristalsis begins, 5 esophageal sphincter allow food to pass, 6,4,10, 9,7,8

Explanation:

How does a catalyst influence a chemical reaction?

Answers

Answer: A catalyst speeds up a chemical reaction, without being consumed by the reaction. It increases the reaction rate by lowering the activation energy for a reaction. ... Remember that with a catalyst, the average kinetic energy of the molecules remains the same but the required energy decreases

Explanation:

If a splinter of wood were to enter the skin and introduce microorganisms to that site, what specific early and late events of inflammation would respond to that newly infected tissue

Answers

Answer:

If a splinter of wood enters the skin and introduces microorganisms to the site the body would first release chemical mediators like histamine then cause vasoconstriction. Platelets work to make a blood clot then neutrophils come in and vacillation occurs. This causes redness, warmth, swelling and pain. Edema and pus formation occurs therefore the neutrophils work to rid the area of cell debris and pathogens. Later the lymphocytes and macrophages will finish the job of the neutrophils and scar tissue is formed to repair the damaged tissue.

Explanation:

In the wild, various sperm and egg cells must navigate a complex environment to complete fertilization. There are mechanisms in place at the molecular level to guard against interspecies fertilization, even between very closely related species. Some of these mechanisms involve lock and key functions of interacting proteins. These molecules most likely regulate which type of reproductive isolation mechanism? Please explain.

Answers

Answer:

pre-zygotic reproductive isolation

Explanation:

The molecules regulate the pre-zygotic reproductive isolation mechanism.

Reproductive isolation generally refers to the variety of biological processes that sustain the formation of new species of biological organisms.

Some of these processes act to prevent fertilization or the formation of zygotes between organisms that are not of the same species (pre-zygotic mechanisms) while others ensure that the product of fertilization is invalid in case the former fails (post-zygotic mechanisms).

Some pre-zygotic mechanisms ensure that there exists no form of mating among organisms that are not of the same species due to incompatible reproductive organs, but where this fails, another pre-zygotic mechanism will act so that that the mating will not lead to fertilization and there will not be formation of zygotes.

The prevention of fertilization between organisms with compatible reproductive organs but of different species is carried out by molecules. These molecules ensure that there are locks and keys in place to prevent the fertilization of the egg by the sperm.

A karyotype shows that a child has Klinefelter syndrome (47,XXY). If the child is also color-blind (due to a recessive X-linked allele), despite his parents having normal color vision, in which parent and stage of meiosis did nondisjunction occur?

Answers

Answer:

It occurred in the mother and in meiosis II stage

Explanation:

A karyotype shows that a child has Klinefelter syndrome (47,XXY) which involves a male child having an XXY chromosome in which the XX chromosome codes from the mother.

If the child is also color-blind (due to a recessive X-linked allele), despite his parents having normal color vision then the nondisjunction occurred in meiosis II because sister chromatids separate during meiosis II and the non separation is the reason for the Klinefelter syndrome.

Two students performed the same experiment, testing how far iodine will diffuse through starchy tissue, such as potatoes. They cut 5 potato squares each, all of equal sizes, and placed them into small cups with iodine solution for 15 minutes. Then they measured how far from the edge of the potato the iodine diffused into the potatoes, in millimeters
Distance Diffused (mm)
Trial 1 Trial 2 Trial 3 Trial 4 Trial 5
Student 1 3.25 3.17 3.26 3.64 3.44
Student 2 3.14 3.43 3.22 3.37 3.28
What is the range of student 1's results?
A. 3.35
B. 0.29
C.0.47
D. 3.29

Answers

Answer:

0.19

Explanation:

3.44-3.25 = 0.19

Is B supposted to be 0.29 or 0.19?

The the range of student 1's results is 0.47. The correct option is C.

What is testing?

An experiment is a test in which a system's component is changed to examine how the result is affected.

In order to determine the exact source of the experimental outcomes, tests should ideally involve manipulating as many additional variables as possible.

The process of scientific testing is determining what we would expect to see if a theory were true and contrasting it with what we actually see.

Through testing and experimentation, the scientific method establishes facts in an unbiased manner.

Making an observation, formulating a hypothesis, making a prediction, carrying out an experiment, and then evaluating the findings are the fundamental steps.

The range of student 1’s result is equal to = 3.64 - 3.17 = = 0.47

Thus, the correct option is C.

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Proteins are NOT found in what type of food source?​

Answers

Answer:

all fruit except dried fruits

all vegetables except peas, beans and corn

herbs and spices

Primatologists Group of answer choices know nothing of primate bones. study only physical aspect of primates. explore the relationships between specific social behaviors and reproductive fitness. study cultural anthropology.

Answers

Answer:

The correct option will be Option B (study only physical aspect of primates).

Explanation:

The significant components of that same endangered as well as surviving primates are analyzed by a primatologist. Also, the primates come to terms with either the molecular and physiological elements. A primer broader knows a great deal about everything from the bones of the primates. We also integrated depth of understanding of both biologizes including primate behavior.

The other choices don't apply to the specified scenario. So option B will be the perfect option for b.


How are the current and resistance related when the voltage of a circuit is constant?
The current doubles when the resistance doubles because they are directly proportional.
The current doubles when the resistance doubles because they are inversely proportional.
The current is cut in half when the resistance doubles because they are directly proportional.
O The current is cut in half when the resistance doubles because they are inversely proportional.

Answers

Answer:

The current is cut in half when the resistance doubles because they are inversely proportional.

Explanation:

Ohm's law states that the electrical current (I) flowing in a circuit is proportional to the voltage (V) and inversely proportional to the resistance (R).

I = V/R

Similarly, increasing the resistance of the circuit will lower the current flow if the voltage is not changed.

HOPE IT HELPS.

Answer:

D

Explanation:

Why were the advent of metagenomics, and the application of molecular techniques such as single-cell and next-generation sequencing, important for defining a microbial relationship

Answers

Answer:

Based on novel approaches, it has been seen that symbiotic associations prevails between a host and one to thousands of more microbes, which challenges the previous meaning of symbiotic association as one that takes place between the host and a single microbe. It was considered previously that symbiotic association’s takes place only between the pair of single microbe and host.  

However, newer approaches has revealed that such kind of association can take place between one microbe and many other hosts. A symbiotic association can take place between microbe-microbe, plants-plants, microbe-plants, and others. However, symbiotic association has never been found to take place between a bacteria and animal host. The symbiotic associations are always not parasitic.  

20. Geneticists creating the "glow in the dark" or fluorescent rabbit, inserted the GFP gene
from a jellyfish that makes it glow. The geneticist studied these rabbits to learn how....

(1 point)
A)to create a new energy source

B)to create interesting looking pets.

C) the florescent cells can help track the movement of cells.

D) to create light in a dark laboratory.

Answers

The correct answer is C) the fluorescent cells can help track the movement of cells.

Explanation:

In the last years, geneticists and scientists created animals that glow in the dark by inserting a Green Fluorescent Protein or GFP gene found in some species of jellyfish. This protein was used in animals such as rabbits, rats, and even chickens. One of the key reasons for this is that by inserting fluorescence scientists can better observe the development and movement of cells. This includes analyzing cells reproduction and growing in embryos of "glowing" animals or inserting the protein in specific cells or organs in an organism to observe how these change or move. Thus, the purpose of studying fluorescent rabbits is that "the fluorescent cells can help track the movement of cells".

Which type of muscle tissue is both voluntary and striated?

smooth
cardiac
skeletal
heart

Answers

Answer:

skeletal muscle fibres.

Explanation:

skeletal muscle fibres occur in muscles which are attached to the skeleton. They are straited in appearance and under voluntary control.

Hope it helps.

Answer:

C

Explanation:

Hope that helps!

Emily and Zach are two students working in the lab to improve the stability and activity of the newly discovered transport protein BCM1 in eukaryotic cells. While introducing mutations into the protein sequence that were predicted to improve the stability and activity of BCM1 Zach convinced Emily to mutate some of the amino acids in the N-terminal signal peptide region of the protein sequence. Subsequently, they expressed this mutated BCM1 protein in eukaryotic cells and observed very little transport across the membrane via the mutate BCM1 transporter in comparison to the levels of transport that was observed via the unmutated BCM1 transporter. Upon further analysis Emily determined that the reduction in transport across the mutated BCM1 transporter was due to mutations made in the signal peptide region of the BCM1 sequence. Explain to Zach the importance of this signal peptide region and disrupting it would lead to reductions in the BCM1 transporter activity.

Answers

Answer:

A mutation in the N-terminal region may alter protein stability  

Explanation:

Transport proteins are proteins capable of transporting substances through biological membranes. These proteins are located within cellular membranes where they form channels that allow the movement of substances between the internal and external sides of the membrane. The N-terminus is the first region in the protein that emerges from the ribosome during its synthesis. This region is usually composed of signal peptides consisting of about 30 amino acids required for protein delivery. Moreover, the N-terminal region is also important because it dictates protein degradation by peptidases. Consequently, mutations in the N-terminal region of transport proteins can alter the properties of these proteins, i.e., either by modifying protein stability or by altering protein signaling.

Which of the following formed in response to the cooling climate of the Archean? a. lava b. rocks c. fossil fuels d. oceans

Answers

Answer:

It is oceans I got it wrong on my test

Explanation:

The following is formed in response to the cooling climate of the Archean: rocks that are present in Option B, the Archean eon is a geological period where the earth's climate was cooling and the first continents were beginning to form.

What exactly is the Archean eon?

During the Archean eon, the Earth's climate was gradually cooling due to a number of factors, such as a decrease in the amount of volcanic activity, which released greenhouse gases into the atmosphere and helped to maintain a warmer climate, a variety of rocks began to form during this archean eon, such as igneous rocks, which are formed from the solidification of molten rock, and metamorphic rocks, which are formed through the alteration of pre-existing rocks.

Hence, the following is formed in response to the cooling climate of the Archean: rocks that are present in option b.

Learn more about the Archean era here.

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The pancreas secretes the hormones insulin and glucagon for homeostatic control of ______ levels in the blood. A. Glucose B. Sodium C. Potassium D. Lipids E. Amino Acid

Answers

They help control the A. Glucose levels in the blood

the fact that for a given species the amount of purnies in hte dna always matches the number of pyrimidines was first determined by

Answers

Answer:

It was given by Chargaff and it is know as chargaff's rule

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