A common method for analyzing microtubule motor activity is to attach them to a glass coverslip (the tails stick very well to glass) and then incubate with microtubules, which will settle on them and then "glide" across the surface of the coverslip as the heads of the motors propel them. This is easily observable in the light microscope. In an assay such as this, how can one determine the directionality of the movement of the microtubules (plus- or minus-ended)? Assume that you do not know if the motor is a plus- or minus-end motor prior to the experiment.

Answers

Answer 1

Answer:

Explanation:

Microtubules are integral part of the cytoskeleton of eukaryotic cells, responsible for maintaining the shape of the cell. They are involved in intracellular trafficking by forming veritable "rails" along which motor proteins move and transport various organelles.

In analyzing microtubular motor activity, the plus (+) end and the minus(-) end proteins can be marked with non-identical fluorescent proteins, followed by observing it under the fluorescent microscope. From observation, the presence of motility of the fluorescent dye helps to determine if the motor is plus or minus end.


Related Questions

Process where gases in the atmosphere trap heat and regulate the earth's temperature

Answers

Answer:

Green house effect.

The greenhouse effect is a process that occurs when gases in Earth's atmosphere trap the Sun's heat. This process makes Earth much warmer than it would be without an atmosphere. The greenhouse effect is one of the things that makes Earth a comfortable place to live.

Answer:

it is the natural process that warms the Earth's surface. The process is called the greenhouse effect because the exchange of incoming and outgoing radiation that warms the planet works in a similar way to a greenhouse

Explanation:

Scientists attribute the global warming trend observed since the mid-20th century to the human expansion of the "greenhouse effect"1 — warming that results when the atmosphere traps heat radiating from Earth toward space.

Certain gases in the atmosphere block heat from escaping. Long-lived gases that remain semi-permanently in the atmosphere and do not respond physically or chemically to changes in temperature are described as "forcing" climate change. Gases, such as water vapor, which respond physically or chemically to changes in temperature are seen as "feedbacks.

Water vapor. The most abundant greenhouse gas, but importantly, it acts as a feedback to the climate. Water vapor increases as the Earth's atmosphere warms, but so does the possibility of clouds and precipitation, making these some of the most important feedback mechanisms to the greenhouse effect.

Carbon dioxide (CO2). A minor but very important component of the atmosphere, carbon dioxide is released through natural processes such as respiration and volcano eruptions and through human activities such as deforestation, land use changes, and burning fossil fuels. Humans have increased atmospheric CO2 concentration by 47% since the Industrial Revolution began. This is the most important long-lived "forcing" of climate change.

Methane. A hydrocarbon gas produced both through natural sources and human activities, including the decomposition of wastes in landfills, agriculture, and especially rice cultivation, as well as ruminant digestion and manure management associated with domestic livestock. On a molecule-for-molecule basis, methane is a far more active greenhouse gas than carbon dioxide, but also one which is much less abundant in the atmosphere.

Nitrous oxide. A powerful greenhouse gas produced by soil cultivation practices, especially the use of commercial and organic fertilizers, fossil fuel combustion, nitric acid production, and biomass burning.

Chlorofluorocarbons (CFCs). Synthetic compounds entirely of industrial origin used in a number of applications, but now largely regulated in production and release to the atmosphere by international agreement for their ability to contribute to destruction of the ozone layer. They are also greenhouse gases.

Nutritionists know that lipids are a vital nutrient that helps keep cells working properly. What is one way the cells in our bodies use lipids?
A to make amino acids
B to repair broken bones
C to form cell membranes
D to carry information in the cell

Answers

A to make amino acids

Which correctly lists three characteristics that are used to describe air? altitude, density, radiation O density, radiation, mass Omass, pressure, density Opressure, altitude, radiation​

Answers

Answer:

The Correct Answer Is C

Explanation:

All Three Aspects are related to Air.

Air has mass, applies pressure, and takes up space. Therefore, option (C) describes the characteristics of air.

What is air?

Air has particular qualities or features and is a mixture of gases, water vapor, and other elements. Gases make up air, which has mass, pressure, and weight as well as the ability to be compressed and be affected by temperature.

Nearly all of the air is made up of the molecules of two separate elements, nitrogen and oxygen. Carbon dioxide and argon, in trace levels, make up the remainder. (Trace amounts of other gases, including neon, helium, and methane, are also present.)

Learn more about air, here:

https://brainly.com/question/1698086

#SPJ6

You have a cup of beans in front of you with 20 white beans and 20 red beans. These beans represent the alleles for a simple Mendelian trait, where red is dominant (R) and white is recessive (r). The cup holds the alleles for an entire population (the gene pool). Note: You may substitute other everyday objects (such as candies, coins, or paper clips) for the beans, but be sure to designate which objects represent the dominant versus recessive alleles.
How many alleles of each type are present in your overall population?

Answers

Answer:

Dominant: 20, Recessive: 20

Explanation:

The case shown in the question above explores simple Mendelian traits, since it shows a population where individuals of the same species present the dominant allele (R- red) and the recessive allele (r- white). We can project, then, that this population has an allele balance, where it is possible to find 20 red beans, which have the dominant allele and 20 white beans, which present the recessive allele.

3. Where does the carbon dioxide for photosynthesis come from?

Answers

Answer :
the air

Explanation:

The carbon dioxide required for photosynthesis comes from the air. It enters leaves through the stomata . Water enters the plant through the roots, and is transported to the leaves in the xylem .

How does all 4 of earths spheres interact in a event

Answers

Answer:

These spheres are closely connected. For example, many birds (biosphere) fly through the air (atmosphere), while water (hydrosphere) often flows through the soil (lithosphere). ... Events can occur naturally, such as an earthquake or a hurricane, or they can be caused by humans, such as an oil spill or air pollution.

Explanation:

Equipment required: A hair dryer, two glass beakers, glasses or glass jars, two thermometers, and enough water to fill one of the beakers or glasses.

Fill one beaker with tap water at room temperature and leave the other one empty. Place a thermometer in each beaker. Set the two beakers side-by-side and record the temperatures in each beaker. Using the hair dryer, blow hot air evenly for several minutes on the two beakers and monitor the temperatures in each beaker. How do the two beakers respond to the hot air?

Answers

Answer:

The temperature of the beaker with water should change very little compared with the beaker with just air, demonstrating that the heat capacity of water can resist changes in air temperature.

Explanation: EDMENTUM/PLATO ANSWER

The two beakers unequally respond to the hot air. This is because the beaker full of water has a heat holding capacity that can resist the alteration in the temperature when hot air is blown onto it.

What is Thermometer?

A Thermometer may be defined as an instrument that can measure the temperature of a particular location significantly.

According to the question, the temperature of the beaker which is full of water changes very little as compared to the beaker which is empty.

Therefore, it is well described above.

To learn more about Thermometers, refer to the link:

https://brainly.com/question/2339046

#SPJ2

36. Why is it important to understand the relationships among animals?

Answers

Answer:

because they have feeling and we are animals ourself

Explanation:

.

Answer:

to know how to protect ecosystem

Explanation:

Based on the phylogenetic tree, which two species are most closely related?




A. Alpaca and blue whale

B. Wild boar and Angus cattle

C. Angus cattle and white-tailed deer

D. Pygmy hippopotamus and white-tailed deer

Answers

Answer: A

Explanation: it just looks like they have a good connection and you can see the line for the alpaca go’s to the wild boar

Please mark me brainless I’ve never been marked before.

/ \
OwO

Depending on the phylogenetic tree, the two species that are most closely related are option c. Angus cattle and white-tailed deer.

Relation of two species:

Angus cattle and white-tailed deer should be considered as the two species as these are closely related since it should both be taxa species that represent the close relationship in the phylogenetic tree.

Also, phgmy hippotamus and while tail dear should be less closely related as compared to the option c.

Hence, the option c is correct.

Learn more about species here: https://brainly.com/question/15130866

A student desined an experiment to test the affect of salt of the number of plant that lived in the water. what is the independent variable in the experiment

Answers

The independent variable should be the plants

What is the basic unit of all living things?

tissue
organ system

organ

cell

Answers

Answer:

The cell is the basic unit of all living things.

Answer:

cell

Explanation:

Took the test

Would an atom with high electronegativity more likely react with an atom with high ionization energy or low ionization energy?? Why???

Answers

Answer:

a high ionization atom

Explanation:

Answer

It would be more likely to react with an atom with low ionization energy. Electronegativity refers to an atoms ability to attract electrons to it. If it has a high electronegativity, it has an easier time attracting electrons. This is important when it comes to forming bonds in which electrons are being shared (like in covalent bonds).

hope this answers your question! :)

Helpppppppp!!! I need help please explain this to me.

Answers

First is solute then solvent for the second I think

Answer:

i can kiss you?

Explanation:

For question 3 please :(

Answers

decomposers i took the test and got it right

Answer:

Secondary consumer

Explanation:

The algae is the producer.

The fish eats the algae first, so it's the primary consumer.

The eagle eats the fish which had eaten algae, so the eagle is the secondary consumer.

Hope that didn't confuse you ;D

Hope this helped :)

Spermatozoa and ovum are specialized cells that contain half the number of chromosomes from both parents. The spermatozoa and ovum combine chromosomes to create a fertilized egg with a full set of chromosomes.

Which process creates spermatozoa and ovum?


cell differentiation

cell growth

meiosis

mitosis

Answers

Answer:

c

Explanation:

The effects of anaerobic conditions

Answers

Answer:

Under anaerobic conditions (a lack of oxygen), the conversion of pyruvate to acetyl CoA stops. In the absence of oxygen, electron transport stops. NADH is no longer converted to NAD+, which is needed for the first three stages of cellular respiration.

Explanation:

lmk if I did anything wrong

Answer:

The effects of anoxia on water and solute transport across excised roots of young maize plants (Zea mays L. cv. Tanker) grown hydroponically have been studied. With the aid of the root pressure probe, root pressure (Pr), root hydraulic conductivity (Lpr), and root permeability (Psr), and reflection (σ sr) coefficients were measured using potassium nitrate (a typical nutrient salt) and sodium nitrate (an atypical nutrient salt) as solutes. During a period of 10–15 h, anaerobic treatment (0.0–0.2 g O2·m-3 in root medium) caused a decrease of root pressure by 0.01–0.28 MPa (by 10–80% of original root pressure) after a short transient increase. For a time period of 5 h, the decrease in the stationary root pressure was not reversible. Under anaerobic conditions, roots still behaved like osmometers and were not leaky. The root hydraulic conductivity measured in osmotic experiments (osmotic solute: NaNO3) was smaller by one to two orders of magnitude than that measured in the presence of hydrostatic gradients. Both the osmotic and hydrostatic hydraulic conductivity decreased during anaerobic treatment by 28 and 44%, respectively, at a constant reflection coefficient of the solutes (σ sr=0.3−1.0). As with root pressure, changes in root permeability to water and solutes were not reversible within 5 h. Under aerobic conditions and at low external concentrations (31–59 mOsmol·kg-1), osmotic response curves were monophasic for KNO3, i.e. there was no passive uptake of solutes. Response curves became biphasic at higher concentrations (100–150 mOsmol·kg-1)- For NaNO3, response curves were biphasic at all concentrations. Presumably, this pattern was a consequence of the fact that potassium had already accumulated in the xylem. During anoxia, accumulation of potassium in the xylem was reduced, and biphasic responses were also obtained at lower potassium concentrations applied to the medium. The results are discussed in terms of a pump/leak model of the root in which anoxia affects both the active ion pumping and the permeability of the root to nutrient salts (leakage). The effects of anaerobiosis on the passive transport properties of the root (Lpr, Psr, σ sr) are in line with the recently proposed ‘composite transport model of the root’.

Identify 5 adaptations (anatomical and/or behavioral) these organisms have
that allow them to survive in that biome.
Ex. modified glands to get rid of excess salts. In Galveston bay tx

Answers

Answer:

Explanation:

Fish- Gills, allows to breathe under water

Shark- Small receptors on their heads allow for magnetic field sensing

Beavers- Slaps their tails when a animalgets close to their dam

Shrike Bird- Catches bugs and sticks them on thorns to impress potential mates

Birds Beaks- Birds have adapted to eat many different foods, their beaks show what kind of food they eat

Explain what you think would happen to an egg placed in salt water? Describe why this would occur.

Answers

Answer:

An egg placed in salt water will float!

Explanation:

In normal tap water, an egg will sink because it's density is greater than the density of water. But, when salt is added to the water, it's density becomes greater than the egg's density. The term used to describe this is "buoyancy."

What is the digestive hormone called cholecystokinin

Answers

Answer:

Answer is bile

Cholecystokinin is secreted by cells of the upper small intestine. Its secretion is stimulated by the introduction of hydrochloric acid, amino acids, or fatty acids into the stomach or duodenum. Cholecystokinin stimulates the gallbladder to contract and release stored bile into the intestine.

Which of the following statements regarding the human
population are TRUE? Select TWO.
SELECT ALL THAT APPLY
The world's population of humans is
increasing.
In every country, the human population is
increasing.
In some countries, the human population is
decreasing or staying the same.
The world's population of humans is
staying constant.

Answers

Answer:

The first two

Explanation:

In most places the population is going up

A piece of steel has a mass of 1170g and a volume of 150 cm3. What is the density of
steel?*

Answers

Answer:

The answer is 7.80 g/cm³

Explanation:

The density of a substance can be found by using the formula

[tex]density = \frac{mass}{volume} \\[/tex]

From the question

mass of metal = 1170 g

volume = 150 cm³

We have

[tex]density = \frac{1170}{150} = \frac{117}{15} = \frac{39}{5} \\ [/tex]

We have the final answer as

7.80 g/cm³

Hope this helps you

How do limiting factors most affect population size?
3
stop population growth
O restrict population growth
O increase population growth
O decrease population growth

Answers

Answer:

restrict population growth

Answer:

b.

Explanation:

Your spinal cord is part of your central nervous system.
True or false?

Answers

Answer:

The answer is True the spinal cord is part of your Central Nervous system, or CNS.


Label the 4 Layers of the Food Web by placing the correct organism in the correct location using the word bank provided to you
1.
Bass Fish


Perch Fish


Zooplankton


Water Lilly


Plz help me out and hurry thank you

Answers

Bottom layer: Zooplankton
Second bottom layer: Water Lilly
Third bottom layer: Perch fish
Top layer: Bass fish

Write a sentence using the word “Satellite”.

Answers

The Sputnik 1 was the first satellite sent up to space.

Answer:

jamie looked out the window of her spacecraft The Wanderer, just to see an old rememece of Earth, a satellite.

Explanation:

Question 6 of 10
Pure liquid water has a pH of
O A. 8
O B. 9
O C. 7
O D. 6

Answers

Answer :
C. 7

Pure liquid water has a pH of 7.

11. Calculate the velocity of a mountain climber if that climber is moving southwest
at a pace of 1.6 km in 1.4 hours?

Answers

Answer:

1.1 km/h northeast

Explanation:

diamonds are very dense material predict what would happen to the light ray if you projected it from air through a diamond.

Answers

Answer:

If a light ray projected through a diamond, the light would refract snd it would act almost like a prism. It would make a rainbow, and the light would break up and disappear

Identify the discovery that contributed to the radio

Answers

I am in the middle of the day I have a good time and you can do that a little later I will get back with me later today I am going out of the way for the next two days so that you have to have your proof that I was going on the last day I am in a meeting at my office in admin office I have a meeting at my house at my meeting and then we have a couple questions about how you are going and how the answer is c

in which situation would osmosis most likely occur

Answers

Answer:

Osmosis occurs until the concentration gradient of water goes to zero or until the hydrostatic pressure of the water balances the osmotic pressure. Osmosis occurs when there is a concentration gradient of a solute within a solution, but the membrane does not allow diffusion of the solute.

Explanation:

Other Questions
graph the solution to the inquality 2x+355 please help me solve this inquality i think i need to solve for x Anwser these questions for 50 points Use a proportion to convert the measurement.10 yards =_______meters Apply to a Successful fitness program PLEASE HELP!!! ILL GIVE BRANLIEST *EXTRA POINTS* dont skip :(( The bottom of Paul's fish tank is a square shape that has an area of 64 square inches.Paul wants to buy a decorative square plate to put in the bottom of the tank. Whatshould be the length of the sides of the plate that Paul buys? what was the main reason the oigrims gave for creating the mayflower compact If Steve paid back $7,945 on a $7000 simple interest loan after 3years. What was the interest rate? The word "spunk can also mean courage, bravery, or determination. Which character do you think has the most spunk? Why True or False: every place on earth has a location on theplanet's surface A square patio has an area of 180 square feet. How long is each side of the patio to the nearest tenth?Each side of the patio is approximatelyfeet long I have a picture I really need help with math please someone What is a political party, how many are there,and how do they communicate their beliefs? Which details in the exposition of the story indicate why mr.sasaki hires teruo page 462 ?????????????????????? Help???plzz What do the sensors in our bodies monitor ? How does and adolescent's thinking differ from that of a child? According to historian Pauline Maier in Liberty, the great paradox that needs to be explained is:A.) That despite the warnings from Parliament, colonists did not understand that their actions would lead to a revolution and this was owing to the difficulty in communication.B.) How in a mere thirteen years, colonists went from being proudly and optimistically British to declaring their independence and that they did was unexpected to all who lived during that time. C.) That abolitionists in the northern colonies would united with slave holders in the southern colonies to fight for independence and the natural rights of man.D.) That the elites were in charge of seeking independence and forced the working classes to join in the effort against their will and interests ........... is the fields of ice that have built up over millions of years. Which ordered pair best represents the location of the y-intercept?A)(2.5, 0)B)(0, 2.5)C)(-5, 0)D)(0, -5)