The hypothesis is that the enzyme was not purified successfully during the last step. It is possible that the sample contained other proteins that were not removed and they are obscuring the target enzyme. It is also possible that the target enzyme was denatured during the last step. You could confirm this hypothesis by running a western blot with a specific antibody against the target enzyme.
Example of making hypothesisMy hypothesis would be that the enzyme was not successfully purified during the last step of the process. This could be due to a variety of factors, such as the enzyme not binding to the purification column or being lost during the elution process.
It is also possible that the enzyme was degraded or damaged during the purification process, leading to its absence from the SDS gel. Further investigation would be needed to determine the exact cause of the enzyme's absence from the gel.
Learn more about SDS gel at
https://brainly.com/question/30558953
#SPJ11
T/F The odontoblast process develops at the proximal end of the odontoblast, adjacent to the dentinoenamel junction. Gradually, the cell moves pulp-ward, and the odontoblast process elongates.
The statement 'The odontoblast process develops at the proximal end of the odontoblast, adjacent to the dentinoenamel junction. Gradually, the cell moves pulp-ward, and the odontoblast process elongates.' is False because the odontoblast process actually develops at the distal end of the odontoblast, adjacent to the predentin.
The odontoblast processes have a role in mechanosensation, dentin healing in mature teeth, and the secretion, assembly, and mineralization of dentin during development. Its three-dimensional arrangement is poorly understood since they are tiny and closely packed.
Dentinal tubules house the odontoblast process. It develops during dentinogenesis as a portion of the odontoblast stays in place as the main body of the cell migrates towards the pulp chamber of the tooth.
As the odontoblast secretes dentin, it gradually moves pulp-ward, and the odontoblast process elongates.
The odontoblast process is responsible for maintaining the vitality of the dentin, and is involved in the formation of dentinal tubules.
To know more about odontoblast process here:
https://brainly.com/question/28260094#
#SPJ11
What staining is used to show apoptotic nuclei? DAPI TUNEL a-actin Question 20 What is the challenge of iPS cell therapy? No ethical issues Results in teratoma formation Immune response not an issue.
The staining that used to show apoptotic nuclei is the TUNEL staining (option 2)
TUNEL staining is a way of measuring DNA fragmentation (apoptotic cells) through the incorporation of labeled nucleotides using the enzyme TdT (terminal deoxynucleotidyl transferase). The incorporated nucleotides bind to fragmented DNA in cells undergoing apoptosis, making it easy to detect the cells under a microscope.The other two options, DAPI and α-actin, are not used to show apoptotic nuclei. DAPI is used to stain DNA while α-actin is used to stain muscle cells.
Therefore, the correct answer is TUNEL staining (option 2)
The challenge of iPS cell therapy is teratoma formation (option 2)
Induced pluripotent stem cell (iPS) therapy involves using mature cells from a patient's own body to reprogram them into a state similar to embryonic stem cells (ESCs). These iPS cells may then be differentiated into any cell type, providing a supply of new cells for regenerative medicine without the ethical concerns connected with ESCs.
Therefore, the correct answer is the result in teratoma formation (option 2)
Learn more about apoptotic here: https://brainly.com/question/27584093
#SPJ11
Use the Nernst equation to calculate the equilibrium membrane potential for K, Na, Ca, and Cl, at room temperature, where:
a) [K]i = 100, [K]o = 5
b) [Na]i = 20, [Na]o = 120
c) [Ca]i = 0.1, [Ca]o = 10
d) [Cl]i = 5, [Cl]o = 120
e) Explain or Define what the Nernst Equilibrium Potential is (in words).
The equilibrium membrane potential for a)K, b)Na, c)Ca, and d)Cl, at room temperature using Nernst equation is a)-77.1 mV, b)62.7 mV, c)-128.4 mV and d)-69.3 mV respectively. The Nernst equation is a mathematical equation used to calculate the equilibrium membrane potential (Em) of a given ion, based on its permeability, its concentration inside and outside the cell, and the temperature.
The Nernst equation is expressed as: Em = (RT/zF) ln ([X]i/[X]o)
Where:
R is the gas constantT is the temperature in Kelvinz is the valence of the ionF is the Faraday constant[X]i is the intracellular concentration of the ion[X]o is the extracellular concentration of the ionIn order to calculate the equilibrium membrane potential for K, Na, Ca, and Cl at room temperature, we will use the Nernst equation above and the given concentrations as follows:
K: Em = (8.314 J/Kmol)(293 K)/(1)(96485 C/mol) ln ([100]i/[5]o) = -77.1 mV
Na: Em = (8.314 J/Kmol)(293 K)/(1)(96485 C/mol) ln ([20]i/[120]o) = 62.7 mV
Ca: Em = (8.314 J/Kmol)(293 K)/(2)(96485 C/mol) ln ([0.1]i/[10]o) = -128.4 mV
Cl: Em = (8.314 J/Kmol)(293 K)/(-1)(96485 C/mol) ln ([5]i/[120]o) = -69.3 mV
The Nernst Equilibrium Potential is a measure of the membrane potential at which the net flow of ions across a membrane is zero, resulting in a state of equilibrium. At equilibrium, the concentrations of the ions on both sides of the membrane are equal and the membrane potential remains constant. The Nernst equation can be used to calculate the equilibrium membrane potential for a given ion.
Know more about Nernst equation here:
https://brainly.com/question/13043546
#SPJ11
What is the BEST explanation of how energy is conserved in chemical reactions? A. It is converted or stored, not created or destroyed. B. It is recycled to power reactions, not created anew. C. It is stored in the products of a reaction, not destroyed. D. It is completely transferred to the molecules in the reaction.
My assumption is The energy is stored in the bonds of the products as chemical energy. In an endothermic reaction, the products have more stored chemical energy than the reactants. This is represented by the graph on the left in the Figure below. In an exothermic reaction, the opposite is true. The products have less stored chemical energy than the reactants. You can see this in the graph in the Figure below.
Since the energy contained in the bonds of reactant molecules always equals the energy contained in the bonds of product molecules, the energy a system increases then the energy of the surroundings should decrease by the exact same amount.
so my prediction is that since this energy may only be converted and stored in an object and it is not to change that it would be answer: A
i hope my insight was helpful
Suppose you had a plant cell with a chromosome number of 2n=4 and you knew that the gene for leaf colour was on one pair of chromosomes and the gene for bark smoothness was on a different pair of chromosomes. Use the letters G and H to represent the genes. Draw a chromosome diagram to accurately represent this plant cell during metaphase I of meiosis. Assume that all of the alleles for leaf colour and bark smoothness are recessive.
The chromosome diagram for the plant cell during metaphase I of meiosis would consist of two pairs of homologous chromosomes, with G and H genes located on separate pairs.
During metaphase I of meiosis, the homologous chromosomes pair up and align at the equatorial plane of the cell. In this scenario, the plant cell has a chromosome number of 2n=4, meaning it has two pairs of homologous chromosomes. The gene for leaf color (G) is located on one pair of chromosomes, while the gene for bark smoothness (H) is located on the other pair of chromosomes. Since both genes have recessive alleles, they would be represented by lowercase letters (g and h).
The resulting chromosome diagram would show the two pairs of homologous chromosomes, each with two chromatids. One pair of chromosomes would have the genes G and g, while the other pair would have the genes H and h. The chromosomes would be arranged in a way that the maternal and paternal copies of each chromosome would be adjacent to each other, ready for segregation during meiosis I.
For more questions like Chromosomes visit the link below:
https://brainly.com/question/15128381
#SPJ11
What growth patterns are expected in the plate tests for an organism that is a facultative anaerobe? - Growth in anaerobe Jar plate only - Growth in oxygenated plate only - Growth in both oxygenated and anaerobe jar plates
The growth pattern expected in the plate tests for an organism that is a facultative anaerobe is "growth in both oxygenated and anaerobe jar plates". Thus, Option C holds true.
Facultative anaerobes are organisms that can grow in the presence or absence of oxygen. They are capable of using oxygen for aerobic respiration when it is available, but can switch to anaerobic respiration or fermentation when oxygen is not available.
Therefore, in the plate tests, facultative anaerobes are expected to show growth in both the oxygenated plate and the anaerobe jar plate. This is because they can utilize the oxygen in the oxygenated plate for aerobic respiration, and can switch to anaerobic respiration or fermentation in the anaerobe jar plate where there is no oxygen.
In contrast, obligate anaerobes can only grow in the absence of oxygen and would only show growth in the anaerobe jar plate, while obligate aerobes can only grow in the presence of oxygen and would only show growth in the oxygenated plate.
Learn more about Facultative anaerobes https://brainly.com/question/13628414
#SPJ11
A Cytokine is/areSelect one:a. types of molecules that move between blood epithelial cells by Diapedesisb. molecules that directly kill bacterial cells (a toxic molecule)c. small regulatory molecules released by immune cellsd. a Hormone
A cytokine is a small regulatory molecule released by immune cells. The correct answer is option c. small regulatory molecules released by immune cells.
Cytokines are a group of proteins and peptides that are used in cell signaling. They are released by immune cells in response to a stimulus, such as an infection or injury, and help to coordinate the immune response. Cytokines can have a variety of effects, including promoting inflammation, stimulating the production of immune cells, and regulating the activity of immune cells.
There are many different types of cytokines, including interleukins, interferons, and tumor necrosis factors, each of which has a specific function in the immune response.
Learn more about cytokine here: https://brainly.com/question/17215490.
#SPJ11
at an ATM. Unfortunately, the person who just used that ATM had influenza and sneezed into their hand before touching the buttons. You have transferred the virus to your eyes, and the fluid from your eyes washes into your nasal cavity, where the virus start replicating in you. Which mode of transmission has happened here?
The mode of transmission in this scenario is indirect contact, also known as indirect contact transmission.
The mode of transmission that has happened here is indirect contact transmission. Indirect contact transmission occurs when an individual comes into contact with a contaminated surface, and then transfers the infectious agent to themselves through contact with their eyes, nose, or mouth.
In this case, the contaminated surface was the ATM buttons that the infected person touched before you, and you came into contact with them, transferring the virus to your eyes and ultimately into your nasal cavity. This is a common way that influenza and other respiratory viruses are transmitted, and is why it is important to wash your hands regularly and avoid touching your face.
To know more about indirect contact transmission click here:
https://brainly.com/question/13179769
#SPJ11
Scientist are closely studying how recognizes themselves
Scientists are studying how people recognize themselves through a variety of approaches, including behavioral experiments and brain imaging techniques.
Behavioral Experiments involve asking participants to complete tasks that require self-recognition, such as looking in a mirror or identifying their own voice. These experiments can provide insight on how people may recognize themselves based on auditory and visual cues.
Brain Imaging Techniques, such as MRIs, allow scientists to study the neural processes involved in self-recognition.
A 16-year old female is recently diagnosed with a deficiency of muscle glycogen phosphorylase. Her and her family are concerned and ask the following questions. Based on what you know about skeletal muscle glycogenolysis and muscle metabolism. Please respond to each question with a thoughtful answer that describes the metabolism in these various scenarios.
1. I’m not sure I understand the issue. Can you explain how muscle glycogen is used normally during exercise?
2. I really like to take long walks; will I still be able to do this with my friends?
3. As a result of this deficiency, will I need to get up at night to eat to maintain my blood glucose levels?
4. Do I need to worry about producing excessive lactate during intense anaerobic exercise?
Here are the short answers to the questions above. The explanation for each is below:
During exercise, muscle glycogen is broken down and converted into glucose-6-phosphate. This process is known as glycogenolysis and provides energy for muscle contraction. Yes, you should still be able to take long walks with your friends. However, you may need to adjust your pace or rest more often to avoid exhaustion. Yes, you may need to get up at night to eat to maintain your blood glucose levels. However, your liver also stores glycogen, so most likely you won't have to get up at night.No, as long as you work within your exercise plan.1. Muscle glycogen is a form of glucose that is stored in the muscles. During exercise, the body breaks down this stored glycogen into glucose, which is then used as a source of energy to fuel the muscles. This process is known as glycogenolysis, and it is essential for maintaining energy levels during physical activity.
2. It is possible that you will still be able to take long walks with your friends, but it may be more difficult for you to maintain your energy levels. This is because your body will not be able to break down muscle glycogen as efficiently, and you may experience fatigue more quickly. It is important to speak with your healthcare provider about ways to manage your condition and maintain your activity levels.
3. It is unlikely that you will need to get up at night to eat to maintain your blood glucose levels. This is because the liver also stores glycogen, which can be broken down into glucose to maintain blood sugar levels when needed. However, it is important to follow a healthy diet and work with your healthcare provider to ensure that your blood sugar levels are properly managed.
4. It is possible that you may produce excessive lactate during intense anaerobic exercise. This is because your body will not be able to break down muscle glycogen as efficiently, and may need to rely more on anaerobic metabolism to produce energy. This can lead to an accumulation of lactate, which can cause muscle fatigue and discomfort. It is important to work with your healthcare provider to develop an exercise plan that is appropriate for your condition.
Learn more about glycogenolysis at https://brainly.com/question/13981321
#SPJ11
Explain:When
there is little or no variation in fitness over wide range of
behaviors. Example Screech owls
When there is little or no variation in fitness over a wide range of behaviors, it means that there is little or no difference in the ability of individuals to survive and reproduce, regardless of the behaviors they exhibit. This can occur when the environment is relatively stable and there are no strong selective pressures favoring one behavior over another.
For example, screech owls have a wide range of behaviors, including different hunting strategies and mating behaviors. However, there is little or no variation in fitness among screech owls exhibiting different behaviors.
This means that screech owls with different behaviors are equally likely to survive and reproduce, and there is no selective pressure favoring one behavior over another.
In summary, when there is little or no variation in fitness over a wide range of behaviors, it means that there is no selective pressure favoring one behavior over another, and individuals with different behaviors are equally likely to survive and reproduce.
To know more about Screech owls refer here:
https://brainly.com/question/30059543#
#SPJ11
6. Would you be able to grow a sample obtained from a patient's
wound (suspected to be infected with MRSA) on EMB? Explain.
7. What is the color or TSI for Salmonella?
8. What is a fastidious organism
6. No, it is not possible to grow a sample obtained from a patient's wound suspected to be infected with MRSA on EMB.
7. The color of TSI, or Triple Sugar Iron agar, for Salmonella is red on the slant and yellow in the butt with the production of H2S gas.
8. A fastidious organism is an organism that has complex nutritional requirements and requires specific growth factors or conditions in order to grow.
6. EMB, or Eosin Methylene Blue agar, is a selective and differential medium used to isolate and differentiate between gram-negative bacteria. MRSA, or Methicillin-resistant Staphylococcus aureus, is a gram-positive bacterium and would not be able to grow on EMB.
7. This is because Salmonella ferments glucose and produces hydrogen sulfide gas, but does not ferment lactose or sucrose, which are also present in TSI agar.
8. These organisms are typically difficult to culture in the laboratory and may require special media or growth conditions. Examples of fastidious organisms include Neisseria gonorrhoeae, Haemophilus influenzae, and Streptococcus pneumoniae.
To know more about fastidious organisms click here:
https://brainly.com/question/9415672
#SPJ11
Q5.6. Ruffs are a species of birds whose male members display three different morphotypes related to methods of reproduction. "Independent" males are large and muscular, and aggressively fight each other for territory (and the right to mate with females in that territory). "Satellite" males are small and lean, and linger outside the territory of independent males, hoping to sneak copulation without being noticed. "Faeder" males mimic the outward appearance of females. This tricks independent males into allowing freder males onto their territory, where the faeder males will copulate with the females before being discovered by the independent males. A colony of 500 ruffs invades an isolated island in the middle of the Atlantic Ocean. At the time of settlement, 45 ruffs are AA, 210 are An, and 245 are sa. AA rutes bear an average of 14 offspring each, Aa ruffs bear and average of 10 offspring each, and aa ruffs bear an average of 17 offspring each. All ruffs survive to adulthood.
- AA , Independent morphotype - An , Satellite morphotype - aa , Faeder morphotype Q5.6.1 Make a seatter plot of p and over 30 generations. Q5.6.2 What model of selection is going on in this scenario? How do you know? (i.e. How do the relative fitness values compare to one another)? Q5.6.3 What phenotype is being favored? What benefits does that phenotype have over the alternative possible phenotypes? Q5.6.4 Will this population go to fixation? If so, which allele will become fixed? At which generation did this population reach fixation? (identify the exact generation where the population reached fixation, do not estimate based on the scatterplot you created). What phenotype will all members of the population express after it reaches fixat
The scatter plot cannot be made here as it requires data for 30 generations, which is not provided in the question (Q5.6.1).
The model of selection that is going on in this scenario is disruptive selection. Disruptive selection occurs when extreme phenotypes are favored over intermediate phenotypes. In this case, the AA and aa morphotypes have higher fitness values than the Aa morphotype, indicating that the extreme phenotypes (independent and faeder) are favored over the intermediate phenotype (satellite) (Q5.6.2).
The phenotype that is being favored is the aa (faeder) phenotype, as it has the highest fitness value (average of 17 offspring each). The benefit of this phenotype is that it allows the faeder males to copulate with females without being noticed by the independent males, thus increasing their chances of passing on their genes to the next generation (Q5.6.3)
Yes, this population will go to fixation. The allele that will become fixed is the a allele, as it has the highest fitness value. The exact generation at which the population reaches fixation cannot be determined from the information provided in the question. After the population reaches fixation, all members of the population will express the aa (faeder) phenotype (Q5.6.4).
Learn more about scatter plot https://brainly.com/question/29785227
#SPJ11
Adrián says that since ecosystems are continually changing, succession is a never-ending process. What is the BEST critique of this statement?
A.
It does not specify whether the succession is primary or secondary.
B.
It does not acknowledge that ecosystems can be balanced or stable.
C.
It assumes that both an ecosystem’s biotic and abiotic elements must change.
D.
It suggests that succession is the same in ocean and terrestrial ecosystems.
Adrián says that since ecosystems are continually changing, succession is a never-ending process. It assumes that both an ecosystem’s biotic and abiotic elements must change.- is the best critique of this statement.
What is biotic and abiotic system?
Living creatures known as biologic components have an indirect or direct impact on other species in their surroundings. As an illustration, consider the waste produced by microbes, plants, and mammals.
Abiotic, or non-living, elements, include all chemical and physical parts of an ecosystem. Abiotic elements might differ between various ecotypes and geographical regions. In general, they provide for life. In an ecosystem, they control the quantity, variety, and rate of biotic element population increase. They are referred to be limiting factors as a result.
To learn more about biotic and abiotic system from the given link
https://brainly.com/question/12689972
#SPJ1
What can move, grow react, protect them selves, repair damage, as well as regulate life processes, and reproduce?
The organisms that can move, grow, react, protect themselves, repair damage, regulate life processes, and reproduce are living organisms. These are the characteristics that differentiate living organisms from non-living things.
Living organisms include animals, plants, fungi, and microorganisms. All living organisms have the ability to move, whether it is through locomotion or movement of substances within their bodies. They can also grow and develop, reacting to their environment in order to protect themselves and repair any damage. Living organisms also have the ability to regulate their life processes, such as metabolism and homeostasis, in order to maintain proper functioning. Lastly, living organisms have the ability to reproduce, creating offspring in order to ensure the continuation of their species.
For more such questions on homeostasis, click on:
https://brainly.com/question/1046675
#SPJ11
How do otters impact CO2 levels in the ocean?
Answer: Sea otters help ecosystems capture carbon from the atmosphere and store it as biomass and deep-sea detritus, preventing it from being converted back to carbon dioxide and contributing to climate change. (write this in your own words)
Explanation:
What is the term used to denote a seed coming out of dormancy?
thanks for helping
Answer:
"Germination" is the phrase used to describe a seed emerging from dormancy. Germination is the process through which a plant emerges from a dormant seed or spore. The seed absorbs water and swells during germination, and enzymes within the seed are triggered, breaking down stored food to supply the energy needed for growth. The embryo within the seed then develops, pushing through the seed coat and becoming a seedling. Germination is an important step in a plant's life cycle since it signifies the beginning of its growth and development into a mature plant.
Sources:
Baskin, J. M., & Baskin, C. C. (2014). Seeds: Ecology, biogeography, and evolution of dormancy and germination (2nd ed.). Academic Press.Bewley, J. D., & Black, M. (1994). Seeds: Physiology of development and germination (2nd ed.). Plenum Press.The term
specific activity differs from the term activity in that specific
activity:
is
the activity (enzyme units) in a one gram of protein
is
the activity (enzyme units) in a mi
The term specific activity differs from the term activity in that specific activity is the activity (enzyme units) in a one gram of protein, while activity is the activity (enzyme units) in a milliliter of solution.
Specific activity is a measure of enzyme purity and is used to compare the catalytic activity of different enzymes or the same enzyme from different sources. It is calculated by dividing the enzyme activity by the amount of protein in the sample.
Activity, on the other hand, is a measure of the catalytic activity of an enzyme in a solution. It is usually expressed in enzyme units (EU) or international units (IU), where one unit is the amount of enzyme that catalyzes the conversion of one micromole of substrate per minute under specified conditions. In summary, specific activity is a measure of enzyme purity, while activity is a measure of enzyme catalytic activity in a solution.
Learn more about enzymes at:
https://brainly.com/question/30600790
#SPJ11
An original sample of water containing 7 x 10^6 CFU/ml was diluted by 4 successive 1/10 dilutions. After incubation, 175 colonies were found growing on the plate. How many mls from the last dilution tube were plated out?
The last volume plated out from the last dilution tube would be (10,000/7) ml or approximately 1428.6 ml.
What is a dilution factor?A dilution factor is the factor by which a solution is diluted. To calculate the number of ml from the last dilution tube that were plated out in a given problem, first, we need to calculate the total dilution factor. Then, divide the volume plated by the total dilution factor. The given problem is related to dilution, incubation, and colony-forming units (CFU). The original sample of water has 7 x 10⁶ CFU/ml, which was diluted by 4 successive 1/10 dilutions. After incubation, 175 colonies were found growing on the plate. It is calculated by dividing the volume of the original solution by the volume of the final solution. If the final volume is unknown, the volume of the original solution is divided by the sum of the volume of the original solution and the volume of the diluent.
The dilution factor is calculated by multiplying the dilution of each tube. In the given problem, four 1/10 dilutions were performed. Hence, the total dilution factor would be 1/10 x 1/10 x 1/10 x 1/10 = 1/10,000. Therefore, the dilution factor is 1/10,000.
The volume plated is the volume of the diluted solution that was transferred to the agar plate. In the given problem, the volume of the diluted solution was not given. Hence, we need to calculate the volume plated using the formula:
V1 x C1 = V2 x C2
Where V1 = volume of the original sample,
C1 = concentration of the original sample,
V2 = volume of the diluted solution, and
C2 = concentration of the diluted solution.
Let's assume that the volume of the original sample is 1 ml. Then, the concentration of the original sample is 7 x 10^6 CFU/ml. The dilution factor is 1/10,000. Hence, the concentration of the diluted solution is:
7 x 10^6 CFU/ml x 1/10,000 = 700 CFU/ml.
To obtain 175 colonies, the diluted solution must have contained 175 x 4 = 700 colonies/ml.
Hence, the volume plated would be:
V1 x 7 x 10⁶ CFU/ml
= V2 x 700 CFU/ml
V2 = V1 x 7 x 10⁶/700
V2 = V1 x 10,000/7
Hence, the volume plated out from the last dilution tube would be (10,000/7) ml or approximately 1428.6 ml.
Learn more about dilution here: https://brainly.com/question/24881505
#SPJ11
You are studying the organization of a single-pass transmembrane protein A at the plasma membrane cell membranes were isolated, processed for SDS-PAGE with (Lane 1) or without (Lane 2) treatment with 2-mercaptoethanol (a reducing agent), and the protein was detected by Western blot analysis using a specific antibody. Lane 1 Lane 2 TOP A+A+A A+A A Based on the observed results, answer the following THREE questions to best describe the likely arrangement of protein A at the plasma membrane. Protein A contains three transmembrane domains, and is embedded in the membrane contains one transmembrane domain, and is embedded in the membrane O contains three subunits, one embedded in the membrane, one in the cytoplasm and one in the extracellular medium Question 6 Protein A molecules interact with each other through O Disulphide bonds Non-covalent bonds Partially covalent bonds O Peptide bonds
Protein A contains three subunits, one embedded in the membrane, one in the cytoplasm, and one in the extracellular medium, and these subunits are held together by disulphide bonds.
Based on the observed results, the likely arrangement of protein A at the plasma membrane is that it contains three subunits, one embedded in the membrane, one in the cytoplasm, and one in the extracellular medium. This is because in Lane 1, where the cell membranes were treated with 2-mercaptoethanol, a reducing agent, three separate bands are observed, indicating that the protein is made up of three subunits. In Lane 2, where the cell membranes were not treated with 2-mercaptoethanol, only one band is observed, indicating that the three subunits are held together in the absence of the reducing agent.
Protein A molecules interact with each other through disulphide bonds. This is because 2-mercaptoethanol is a reducing agent that specifically breaks disulphide bonds. The fact that the protein separates into three subunits in the presence of 2-mercaptoethanol indicates that the subunits are held together by disulphide bonds.
Here you can learn more about disulphide bonds
https://brainly.com/question/28431471#
#SPJ11
In 200-250 words answer the following questions:
You have discovered a new animal species! It has quills/spines, and a bill, it is non-venomous, eats mostly ants and termites, and lays eggs. It is a mammal, and its closest relatives are the extant platypus and echidna. Using the principles of parsimony (i.e., the simplest solution), and the cladogram found below, answer the following questions (a-c):
When did placentas evolve? (Select A, B, C, D, E, F, or G)
When did eggs evolve? (Select A, B, C, D, E, F, or G)
Where is the most likely location of the new species you have discovered on the tree? (Select A, B, C, D, E, F, or G)
Is egg laying, a trait found in echidnas and platypuses, a symplesiomorphy, a synapomorphy, an apomorphy? Explain your answer.
Using the principles of parsimony and the cladogram provided, we can answer the following questions:
A) When did placentas evolve?
Placentas evolved at point D on the cladogram. This is because it is the simplest solution, as it is the point where all mammals with placentas (marsupials and placental mammals) diverge from the monotremes, which do not have placentas.
B) When did eggs evolve?
Eggs evolved at point A on the cladogram. This is because it is the simplest solution, as it is the point where all animals on the cladogram diverge from each other. All animals on the cladogram lay eggs, so it is the most parsimonious solution to assume that eggs evolved at the base of the tree.
C) Where is the most likely location of the new species you have discovered on the tree?
The most likely location of the new species on the tree is at point C, between the platypus and echidna. This is because the new species shares characteristics with both the platypus and echidna, such as quills/spines, a bill, and a diet of ants and termites. It is also a mammal, like the platypus and echidna, and its closest relatives are the extant platypus and echidna. Therefore, it is most parsimonious to assume that the new species diverged from the platypus and echidna at point C.
D) Is egg laying, a trait found in echidnas and platypuses, a symplesiomorphy, a synapomorphy, an apomorphy?
Egg laying is a symplesiomorphy, as it is a trait that is shared by all animals on the cladogram and is therefore an ancestral trait. It is not a synapomorphy, as it is not a trait that is shared by a group of animals and used to define that group. It is also not an apomorphy, as it is not a derived trait that is unique to a particular group of animals.
For more question on placentas click on
https://brainly.com/question/1604269
#SPJ11
Q1: Each of the following DNA sequences contains a single prokaryotic protein coding sequence. For each sequence: 1 - Underline the TATA box (TATAAT) in the promoter. The TTGACA at - 35 is not include in these sequences due to space). Assume each gene has an exact match to the consensus sequence
The following DNA sequences each contain a single prokaryotic protein coding sequence: Sequence 1: ATGATACAAATGTTTGGTCTTATAATGTTGTAA. In this sequence, the TATA box (TATAAT) can be found starting at position 13 (ATGATACAAATGTATAATGTTTGGTCTT).
Sequence 2: ATGCCTGATTATCTGAAGCCATGTATGTTGTAA. In this sequence, the TATA box (TATAAT) can be found starting at position 12 (ATGCCTGATTTATAATCTGAAGCCATGTATGTTGTAA). Sequence 3: ATGGGTAAGAGCTTTATAAGTTTTTATAGCGTAA. In this sequence, the TATA box (TATAAT) can be found starting at position 11 (ATGGGTAAGAGTATAATCTTTATAAGTTTTTATAGCGTAA).
The TATA box, or TATA motif, is an essential element of the prokaryotic promoter region and is located upstream of the transcription start site. It is composed of 6 nucleotides (TATAAT) and serves as the binding site for transcription factors. This sequence of nucleotides is also referred to as the core promoter element, as it is essential for the initiation of transcription in prokaryotic cells.
Know more about TATA box here:
https://brainly.com/question/14609156
#SPJ11
Metabolism Question: What are the possible ways for Acetyl-CoA to
leave mitochondria? If ATP-Citrate Lyase is inhibited, is there any
other mechanism for acetyl-CoA to exit the mitochondria?
Acetyl-CoA can leave the mitochondria via the citrate shuttle or carnitine shuttle; if ATP-citrate lyase is inhibited, the acetyl-CoA can still exit via the carnitine shuttle, which involves the transfer of acetyl-CoA to carnitine followed by transport across the inner mitochondrial membrane.
The citrate shuttle involves the conversion of acetyl-CoA to citrate, which can be transported out of the mitochondria and then converted back to acetyl-CoA by ATP-citrate lyase in the cytoplasm. The carnitine shuttle involves the transfer of acetyl-CoA to carnitine followed by transport across the inner mitochondrial membrane via carnitine-acylcarnitine translocase, and subsequent conversion back to acetyl-CoA by carnitine acyltransferase II in the cytoplasm.
Learn more about mitochondria https://brainly.com/question/869305
#SPJ11
Indicate some possible ways in which foods may become
contaminated with enteric organisms- list more than 1 way
Foods can become contaminated with enteric organisms in several ways, including, unsanitary habits during food preparation, such as not washing hands after using the restroom, might introduce enteric organisms into the meal being prepared.
As a result of direct or indirect contact, contaminated foods can spread to other foods, a phenomenon known as cross-contamination.
Vegetables can become contaminated with enteric germs, for instance, if a cutting board is used to chop raw meat and subsequently veggies without sufficient cleaning.
Foods cleaned or prepared with water that is polluted with enteric microbes can be contaminated by eating them.
Expansion and multiplication of enteric microbes can cause food contamination if it is not kept at the correct temperature during storage.
Food can become contaminated if enteric germs are present on the equipment used to prepare or store it.
These are just a few of the possible ways in which foods can become contaminated with enteric organisms. It is important to follow proper food safety practices to prevent contamination and reduce the risk of foodborne illness.
Read more about microbes.
https://brainly.com/question/28166094
#SPJ11
A patient’s lipid profile returned the following results: LDL cholesterol: 185mg/dL HDL cholesterol: 65mg/dL VLDL cholesterol: 14mg/dL LDL+VLDL: 199mg/dL Triglycerides: 36mg/dL Total cholesterol: 265mg/dL i. Which of these parameters are outside of the ranges for a healthy adult? (2 marks, maximum 50 words) ii. Explain why persons with heart disease or hypertension might present with such a lipid profile. (3 marks, maximum 100 words)
Cholesterol is a type of lipid molecule found in the cells of all animals. It plays an important role in the body's metabolism and is used to produce hormones, vitamin D, and bile acids. High levels of cholesterol in the blood can lead to the buildup of plaque in the arteries, increasing the risk of heart disease and stroke.
Learn more about cholesterol at https://brainly.com/question/841110
#SPJ11
HELPP PLEASE ‼️‼️‼️
I have so much other work to get done and it’s due tomorrow!!
HELPPPPPP
WILL MARK BRAINILEST!!!
Au. africanus and Au. robustus has been discovered in a number of South African limestone caverns. Eastern Africa: Sedimentary basins and river drainage systems.
What is Great Rift Valley?The Great Rift Valley is home to numerous australopithecine fossils, including the renowned Au. afarensis. The most well-known Australopithecus fossil discoveries in East and South Africa are probably "Lucy" and "Mrs Ples."
As opposed to what some have previously claimed, Australopithecus fossils from the richest hominin-bearing stratum (Member 4) at Sterkfontein in South Africa are much older and are contemporaneous with Australopithecus afarensis in East Africa. Afarensis exhibited traits common to both apes and humans.
Therefore, Au. africanus and Au. robustus has been discovered in a number of South African limestone caverns. Eastern Africa: Sedimentary basins and river drainage systems.
Learn more about South African on:
https://brainly.com/question/16866117
#SPJ1
Some plants that are grown in an environment with enough nitrogen
and an extended period of darkness, showed the symptoms of nitrogen
defiency. Why did plants show these symptoms?
The reason why some plants showed the symptoms of nitrogen deficiency despite being grown in an environment with enough nitrogen is because of the extended period of darkness they were exposed to. Nitrogen is a crucial nutrient for plants as it is used to create chlorophyll, which is essential for photosynthesis.
However, photosynthesis requires light to occur, and without enough light, the plants cannot use the nitrogen to create chlorophyll and carry out photosynthesis. As a result, the plants exhibit the symptoms of nitrogen deficiency even though there is enough nitrogen present in the environment.
In summary, the extended period of darkness prevented the plants from carrying out photosynthesis, which in turn prevented them from using the available nitrogen and resulted in the symptoms of nitrogen deficiency.
To know more about photosynthesis refer here:
https://brainly.com/question/29764662#
#SPJ11
If you want to detect the expression of protein X in an organism using western blotting and you know only the sequence of protein X, how can you generate the primary antibody, and what kind of secondary antibody can you use?
To detect the expression of protein X in an organism using western blotting, you will need to generate a primary antibody specific to protein X and a secondary antibody that binds to the primary antibody. Here are the steps you can follow:
1. Generate the primary antibody:
- First, you will need to create a peptide or protein that is specific to protein X. This can be done by synthesizing a peptide or protein that corresponds to the sequence of protein X.
- Next, you will need to immunize an animal (such as a rabbit or mouse) with the synthesized peptide or protein. This will stimulate the animal's immune system to produce antibodies against the peptide or protein.
- Finally, you will need to isolate the antibodies from the animal's serum. These antibodies will be specific to protein X and can be used as the primary antibody in western blotting.
2. Choose a secondary antibody:
- The secondary antibody should be specific to the species in which the primary antibody was generated. For example, if the primary antibody was generated in a rabbit, you will need a secondary antibody that is specific to rabbit antibodies.
- The secondary antibody should also be conjugated to a detection molecule, such as an enzyme or fluorescent dye, that will allow you to visualize the presence of protein X on the western blot.
In summary, to detect the expression of protein X using western blotting, you will need to generate a primary antibody specific to protein X by immunizing an animal with a synthesized peptide or protein corresponding to the sequence of protein X, and choose a secondary antibody that is specific to the species in which the primary antibody was generated and is conjugated to a detection molecule.
For more question on western blotting click on
https://brainly.com/question/30587498
#SPJ11
How do you know what altitude a person was killed based off of their blood splatter pattern??
Use blood splatter analysis.
Write 2-6 sentences on how you got your answer.
I Don't Know If You Go Over This In Biology. I Didn't Find Science In The Subject Box.
Forensic Science
Brainliest to whom ever answers first.
Blood spatter analysis can provide information about the direction and velocity of blood, which can help determine the location and nature of the incident, such as the position and movement of the victim and the weapon used.
What is Blood spatter analysis?Blood spatter analysis, also known as bloodstain pattern analysis, is a forensic science technique used to examine the location, shape, size, distribution, and directionality of bloodstains to gain insights into the nature and dynamics of a crime scene.
Blood spatter analysts use scientific methods and tools to interpret the patterns of bloodstains found at a crime scene, including the type of weapon used, the position of the victim and assailant, and the sequence of events leading up to the crime. Blood spatter analysis can be a critical tool in criminal investigations, providing valuable evidence to help reconstruct the events of a crime and identify potential suspects.
Learn more about Blood spatter analysis, here:
https://brainly.com/question/9889637
#SPJ1
defense mechanism when you think teacher is angry at you since your preformed poorly on a test, but teacher isnt actually angry
One common defense mechanism that may be used in this situation is projection. Projection is when an individual projects their own feelings or emotions onto another person, even if that person does not actually hold those feelings or emotions.
In this case, you may be projecting your own feelings of guilt or disappointment about performing poorly on the test onto your teacher, causing you to believe that they are angry at you when they are not.
Another possible defense mechanism in this situation is rationalization. Rationalization is when an individual tries to justify or explain their behavior or feelings in a way that makes them seem less negative or more acceptable. In this case, you may be rationalizing your poor performance on the test by convincing yourself that your teacher is angry at you, even though they are not.
It is important to remember that defense mechanisms are a normal and natural part of human behavior, but they can also lead to distorted perceptions and unhealthy coping mechanisms. It is important to be aware of your defense mechanisms and try to address the underlying emotions or issues that may be causing them.
For more question on projection click on
https://brainly.com/question/4421782
#SPJ11