Why is screening not necessary to select for microbes that have been edited using the Cas9 system?
a. The edited microbes include green fluorescent protein which eliminates the need to grow the cells on a plate containing antibiotic. b. The Cas9 system converts mutant cells to wild-type, which will grow on any media whereas the mutants will not.
c. Microbes that have not repaired the cut by insertion of donor DNA cannot carry out nonhomologous end joining and chromosome breaks are lethal.

Answers

Answer 1

The reason why screening is not necessary to select for microbes that have been edited using the Cas9 system is Microbes that have not repaired the cut by insertion of donor DNA cannot carry out nonhomologous end joining and chromosome breaks are lethal. (C)

This means that only the edited microbes, which have successfully repaired the cut and inserted the donor DNA, will be able to survive and grow.

Therefore, there is no need for additional screening methods, such as growing the cells on a plate containing antibiotic or looking for green fluorescent protein, to select for the edited microbes.

The Cas9 system itself acts as a selection method, as only the successfully edited microbes will be able to survive.

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Related Questions

in
terms of exclusivity and probability, describe how dna analysis
differs from methods such as blood typing and fibgerprinting

Answers

In terms of exclusivity and probability, the dna analysis differs from methods such as blood typing and fingerprinting bacause everyone is unique and has their own character in their genetic material

DNA analysis is more exclusive than blood typing and fingerprinting because it is unique to each individual. While blood typing can only narrow down the identity of an individual to a certain blood group, DNA analysis can pinpoint the exact individual. Similarly, while fingerprints are unique to each individual, they can still have similarities with other individuals' fingerprints, making it less exclusive than DNA analysis.

DNA analysis also has a higher probability of accurately identifying an individual compared to blood typing and fingerprinting. DNA analysis looks at multiple genetic markers, making it more accurate and reliable. Blood typing only looks at one genetic marker, and fingerprinting can be affected by factors such as smudging or partial prints, making them less reliable. In conclusion, DNA analysis is a more exclusive and reliable method of identification compared to blood typing and fingerprinting due to its uniqueness to each individual and its ability to look at multiple genetic markers.

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In the protein adenylate kinase, the c-terminal region is a -helical, with the sequence val- asp-asp- val- phe-ser- gln- val- cys- thr- his- leu- aspthr- leu-lys- the hydrophobic,residues in this sequence are presented in boldface type. Suggest a possible reason for the periodicity in their spacing

Answers

The periodicity of the hydrophobic residues in the c-terminal region of the protein adenylate kinase allows for the formation of a stable alpha helix structure, which is important for the stability and function of the protein.

The protein adenylate kinase has a c-terminal region that is a-helical with a specific sequence of amino acids. The hydrophobic residues in this sequence are spaced periodically and are presented in boldface type. The possible reason for this periodicity in spacing is to allow for the formation of a stable alpha helix structure.

An alpha helix is a common secondary structure in proteins that is formed by the folding of the polypeptide chain into a right-handed helix. The periodicity of the hydrophobic residues allows for the formation of hydrophobic interactions between the side chains of the amino acids, which helps to stabilize the alpha helix structure.

These hydrophobic interactions are important for the stability of the protein and its overall function.

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Question 6 (1 point)
What structures are found lining the small intestine that increase surface area for absorption?
Epiglottis
Villi
Appendix
Salivary gland

Answers

Answer:

I think it is the Villi

Explanation:

I learned in my health science class that the inside of the small intestine has many folds, called the villi. I also did research on the villi and I learned that the villi in anatomy any of the small, slender, cascular projections that increase the surfave area of a membrane. The villi is designed to absorb nutrients from the liquid mixture called chyme produced in the stomach from the food.

The species is blatella
Give details of the external anatomy, 2. Count the number of visible head, thoracic and abdominal segments. 3. Examine the mouthparts and suggest the kind of feeding habit the cockroaches are associated with 4. Examine the antennae and count the number of segments they have. What kind of antennae are they? 5. Examine and draw the different part of mouthparts associated with this insect (Mandible, Maxillae and Labium). How would you describe the dietary modification of mouthparts the roaches have? Suggest the diet of roaches based on their mouthparts. 6. Where are the spiracles located, and how are they distributed on the body? 7. How will you describe the orientation of the mouthpart plane of the mouthparts compared to the plane of the body? Is it prognathous, opisthognathous or hypognathous? 8. Examine the wings are they all alike? How are they different? What kind of wings do they have? 9. Examine the legs, how are they distributed? How many segments do they have? Are the legs biramous or uniramous? Based on the sizes, length of the different parts of legs, what kind legs are they? 10. Describe the terms biramous and uniramous. Give examples of insects/arthropods where they occur. Lab 5b. Internal Morphology of the Insect 1. Cut open on one pleural region of the specimen of cockroach (Blarella) provided. Lift the tergum up and flap it over such that the underlying surface faces upward. 2. Display the internal organs: Respiratory organs - Tracheal system and the spiracles

Answers

The external anatomy of cockroaches includes a flattened and elongated body with a tough exoskeleton.The blatella species has one visible head segment, three visible thoracic segments, and ten visible abdominal segments.Cockroaches are associated with a wide range of feeding habits.Cockroaches have filiform antennae.. The modification of their mouthparts suggests that cockroaches are omnivorous.The mouthparts of the cockroach are hypognathous.The spiracles of cockroaches are located along the sides of their body.The blatella species has tegmina and membranous wings.The legs of cockroaches are uniramous. Biramous legs have two branches, while uniramous legs have only one.

Characteristics of blatella

1. The external anatomy of the blatella species includes a head, thorax, and abdomen. The head contains the mouthparts, antennae, and compound eyes. The thorax contains the wings and legs, while the abdomen contains the spiracles, reproductive organs, and digestive system.
2. The blatella species has one visible head segment, three visible thoracic segments, and ten visible abdominal segments.
3. The mouthparts of the blatella species are adapted for chewing and grinding food. They have mandibles for crushing and grinding, maxillae for manipulating food, and a labium for holding food in place. This suggests that the cockroaches are associated with a generalist feeding habit, meaning they can eat a variety of foods.
4. The antennae of the blatella species have many segments, typically around 100-150. They are filiform antennae, meaning they are long and thin with segments of similar size and shape.
5. The mandibles of the blatella species are strong and adapted for crushing and grinding food. The maxillae are used for manipulating food and the labium is used for holding food in place. The dietary modification of the mouthparts suggests that the roaches have a generalist diet, meaning they can eat a variety of foods.
6. The spiracles are located on the sides of the abdomen and are distributed evenly along the length of the body. There are typically ten pairs of spiracles, one pair on each abdominal segment.
7. The mouthpart plane of the blatella species is hypognathous, meaning the mouthparts are directed downwards
8. The wings of the blatella species are not all alike. The front wings, or tegmina, are thicker and more rigid, while the hind wings are thinner and more flexible. The front wings are used for protection and the hind wings are used for flight. The blatella species has tegmina and membranous wings.
9. The legs of the blatella species are distributed evenly along the thorax, with one pair on each thoracic segment. Each leg has five segments and is uniramous, meaning it is not branched. The legs are cursorial, meaning they are adapted for running.
10. Biramous means that a structure, such as a leg, is branched. An example of an insect with biramous legs is the crayfish. Uniramous means that a structure is not branched. An example of an insect with uniramous legs is the cockroach.

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What are the main differences between nucleic acid and protein
gel electrophoresis? List them down here and discuss well. Give at
least 5 differences

Answers

The main differences between nucleic acid and protein gel electrophoresis is sample preparation, gel matrix, staining methods, size range, and electrophoresis conditions.

What Are The Main Differences Between Nucleic Acid And Protein Gel Electrophoresis?

The main differences between nucleic acid and protein gel electrophoresis are as follows:

Sample preparation: In nucleic acid gel electrophoresis, the sample is usually denatured using heat or chemicals before loading onto the gel. In protein gel electrophoresis, the sample is usually treated with a reducing agent and a detergent to break disulfide bonds and to unfold the protein.Gel matrix: Nucleic acid gel electrophoresis typically uses agarose or polyacrylamide gels, while protein gel electrophoresis typically uses polyacrylamide gels.Staining methods: Nucleic acid gel electrophoresis usually uses ethidium bromide or SYBR Green to visualize the DNA or RNA, while protein gel electrophoresis usually uses Coomassie blue or silver staining to visualize the protein.Size range: Nucleic acid gel electrophoresis can separate DNA or RNA fragments from 50 bp to 50 kb, while protein gel electrophoresis can separate proteins from 5 kDa to 500 kDa.Electrophoresis conditions: Nucleic acid gel electrophoresis is usually performed at a constant voltage, while protein gel electrophoresis is usually performed at a constant current.

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discuss the epithelial barrier and its mechanisms to protect our
body (Cite journals or textbooks please)

Answers

The epithelial barrier and its mechanisms to protect our body from journals or textbooks is tight junctions, mucous secretion, antimicrobial peptides, cilia, and immune cells.

The epithelial barrier is a protective layer of cells that line the surfaces of the body, including the skin, respiratory system, gastrointestinal system, and urinary system. This barrier serves to protect the body from foreign substances, pathogens, and injury.

There are several mechanisms by which the epithelial barrier protects our body, including:

1. Tight junctions: These are protein complexes that form a seal between adjacent epithelial cells, preventing substances from passing between the cells.
2. Mucous secretion: Many epithelial cells produce mucus, a viscous fluid that traps foreign substances and prevents them from reaching the underlying tissues.
3. Antimicrobial peptides: Epithelial cells produce antimicrobial peptides that can kill or inhibit the growth of pathogens.
4. Cilia: Some epithelial cells have hair-like structures called cilia that can move mucus and trapped foreign substances out of the body.
5. Immune cells: Epithelial tissues contain immune cells, such as mast cells and dendritic cells, that can detect and respond to foreign substances.

Overall, the epithelial barrier is an essential part of the body's defense against foreign substances and pathogens. By utilizing a variety of mechanisms, the epithelial barrier helps to protect our body and maintain our health.

Sources:
- Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K., & Walter, P. (2002). Molecular Biology of the Cell (4th ed.). New York: Garland Science.
- Schneeberger, E. E., & Lynch, R. D. (2004). The tight junction: a multifunctional complex. American Journal of Physiology-Cell Physiology, 286(6), C1213-C1228.

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Open Ended Response
33. In a paragraph, discuss the environmental impact of harvesting fossil fuels such as mining and fracking
(natural gas and petroleum), be sure to describe the process of mining reclamation. How can energy
efficiency be improved using renewable energy resources?

Answers

Fossil fuel harvesting methods can have several negative effects on the environment including air and water pollution, emitting greenhouse gases, and producing hazardous waste. Using renewable energy sources, such as solar cells, wind turbines, and hydroelectric power plants can increase energy efficiency.

What is the procedure of Mining reclamation?

Reclamation after mining is the process of returning disturbed land to its original state or a state that is comparable to it. Reclamation after mining is the process of returning land to its pre-mining condition or one that is reasonably close to it. Reclamation after mining is the process of restoring damaged land to its original state or one that is comparable to it. This entails repairing the region's topography, soil, and vegetation as well as taking care of any problems with the water quality brought on by the mining activities. Reclamation aims to bring the land back to a state that is suitable for human use and harmonious with surrounding ecosystems. Reclamation may also involve recovering mined commodities for use in other applications, such as coal, metals, and minerals. The protection of public health and safety, the preservation of natural resources, and the promotion of sustainable development in regions impacted by mining operations all require reclamation efforts, which can be expensive and time-consuming.

How do solar panels increase energy effectiveness?

Capturing solar energy and turning it into useful electricity is how solar panels increase energy efficiency. Appliances, lighting, and other electrical systems can then be run on this electricity. Solar energy systems may provide clean, renewable energy with little negative influence on the environment. They are frequently combined with other renewable energy systems, like wind or hydro, to boost and improve energy production. Solar panel systems can also lower energy costs over time, which makes them a desirable and affordable choice for energy efficiency and conservation.

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Which process involves joining amino acids together into a polypeptide?

-meiosis
-replication
-translation
-transcription

Answers

The translation is the process of combining amino acids to form polypeptides.

What is translation?The translation is the process by which a certain sequence of amino acids to create a polypeptide chain is synthesized using the genetic information stored in messenger RNA (mRNA). Transfer RNA (tRNA) molecules help with this process on ribosomes by bringing certain amino acids to the ribosome and assisting in their assembly into the correct sequence in accordance with the mRNA code.Where does translation occur?On ribosomes, in the cytoplasm of the cell, translation takes place. The genetic code is used by the ribosome during the 5' to 3' reading of the mRNA molecule to identify the order of amino acids that will make up the protein. Each codon on the mRNA is subsequently matched by the ribosome with the associated tRNA carrying the appropriate amino acid. A polypeptide chain is created as the ribosome advances along the mRNA by creating peptide bonds between the amino acids. This procedure goes on until the ribosome encounters a stop codon on the messenger RNA (mRNA), which marks the completion of translation and releases the finished polypeptide chain.

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Is related to the demand for growth and replacement of tissuesOccurs in all adult cells except the Central Nervous System (CNS). is called?

Answers

The process related to the demand for growth and replacement of tissues that occurs in all adult cells except the Central Nervous System (CNS) is called mitosis.

Mitosis is a type of cell division that results in two daughter cells, each having the same number and kind of chromosomes as the parent nucleus. This process is essential for the growth and repair of tissues in the body. However, cells in the Central Nervous System (CNS) do not undergo mitosis, as they are typically unable to regenerate or replace themselves if damaged.A cell prepares for cell division by replicating its chromosomes, segregating them, and creating two identical nuclei during the mitotic phase. The cell's contents are often evenly divided into two daughter cells with identical genomes after mitosis.

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When do cells need glucose? Why? If an organism does not eat glucose, what happens? How is energy stored? How is stored energy released? Which gland and hormones are most responsible for maintaining adequate levels of blood sugar?

Answers

Glucose is a type of sugar and the body's main source of energy passing from the bloodstream into the cells through the hormone insulin.

Cells need glucose when they need to produce energy in the form of ATP. If an organism does not eat glucose it cannot function properly and although it can produce energy through the breakdown of other macromolecules, this is not as efficient and can have negative effects on health and can eventually die.

Energy is stored as glycogen in the liver and muscles and as fat in adipose tissue. The stored energy is released through the process of glycogenolysis.

The pancreas is the gland that maintains blood sugar levels through the production of insulin and glucagon.

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In snapdragons, petal color is determined by a single gene locus with two alleles making the "red" allele (R) incompletely dominant to the "white" allele (r). Heterozygotes have petals, which are neither red nor white, but pink. A) If a true-breeding red flower is pollinated with pollen from a white flower: What fraction of the seeds (FI generation) would be expected to produce red-flowered plants? What fraction of the gametes produced by the El plants would be expected to bear the R allele? b) If two pink flowered plants are crossed, what genotypic and phenotypic ratios are expected among the offspring (F1 generation)?

Answers

The fraction of the F1 generation that is expected to produce red-flowered plants is 0.

As for the fraction of the gametes produced by the F1 plants that would be expected to bear the R allele, it would be 1/2. This is because each F1 plant is heterozygous (Rr) and will produce two types of gametes: R and r.

Out of the four possible offspring, one will be homozygous dominant (RR) and have red petals, two will be heterozygous (Rr) and have pink petals, and one will be homozygous recessive (rr) and have white petals.

A) When a true-breeding red flower (RR) is crossed with a true-breeding white flower (rr), all of the F1 offspring will be heterozygous (Rr) and have pink petals.
B) When two pink flowered plants (Rr) are crossed, the expected genotypic ratio among the F2 offspring is 1:2:1 (RR:Rr:rr) and the expected phenotypic ratio is 1:2:1 (red:pink:white).
This can be visualized with a Punnett square:
 | R | r
--|---|--
R | RR | Rr
r | Rr | rr
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Aspergillus oryzae is a fungus used to saccharify rice, barley, and sweet potato in the process of making alcoholic beverages. Scientists found that evolutionary pressure induced by donestication has caused non-synonymous and gap mutations in genes involving fermentation characteristics. What are these mutations?

Answers

Non-synonymous mutations are changes in the DNA sequence that result in a different amino acid being coded for, which can lead to a change in the protein structure and function.

Gap mutations, also known as frameshift mutations, are insertions or deletions of one or more nucleotides that shift the reading frame of the genetic code, leading to a change in the amino acid sequence and potentially altering the protein's function.

These mutations in Aspergillus oryzae have likely occurred as a result of selective pressure during the domestication process, leading to changes in the genes involved in fermentation characteristics and potentially improving the organism's ability to saccharify rice, barley, and sweet potato for the production of alcoholic beverages.

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label RNA molecule???

Answers

B is the most appropriate answer here as RNA is made of ribose sugar
While A is a phosphate group and
C is a nitrogenous base

Would it be possible for the following types of transporters to
create a concentration gradient across a plasma membrane? Explain
your reasoning!!!
a. ATPase-Pump
b. Symporter
c. Antiporter
d. Unipor

Answers

The following types of transporters to create a concentration gradient across a plasma membrane ATPase-Pump, symporter, antiporter, and unipor. Yes it possible to create a concentration gradient.

ATPase-Pump uses ATP energy to move ions across the membrane against their concentration gradient, which creates a concentration gradient across the plasma membrane. ATPase-Pump is also known as the Sodium-Potassium pump. It is a vital pump for maintaining the concentration of sodium ions outside the cell and potassium ions inside the cell. It helps in creating an electrochemical gradient in which the concentration of sodium ions outside the cell is higher than inside and the concentration of potassium ions inside the cell is higher than outside.

Symporter, this transporter moves two different molecules simultaneously across the membrane in the same direction. This creates a concentration gradient across the membrane as the concentration of these molecules differs inside and outside the cell, example - glucose-Na+ symporter in the intestinal cells. Antiporter, this transporter moves two different molecules simultaneously across the membrane in the opposite direction. This creates a concentration gradient across the membrane as the concentration of these molecules differs inside and outside the cell. Example - Na+/H+ antiporter found in the kidney cells.

Unipor, this transporter moves only one type of molecule across the membrane. It works according to the concentration gradient of that molecule. Example - Aquaporins that move water molecules across the membrane in response to the concentration gradient. Hence, ATPase-Pump, symporter, antiporter, and unipor all can create a concentration gradient across the plasma membrane.

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____ It is a disorder in which tissue that normally lines the uterus, called the endometrium, grows outside the uterine cavity. It can adhere to the outside of the uterus, the ovaries, and the fallopian tubes.

Answers

Endometriosis is a disorder in which tissue that normally lines the uterus, called the endometrium, grows outside the uterine cavity. It can adhere to the outside of the uterus, the ovaries, and the fallopian tubes.

Endometriosis is a condition in which the tissue that normally lines the inside of the uterus, called the endometrium, starts to grow outside of the uterine cavity. This can cause a variety of symptoms, including pelvic pain, painful periods, and infertility.

Endometriosis can affect the outside of the uterus, the ovaries, and the fallopian tubes. It can also affect other organs, such as the bladder and intestines. The exact cause of endometriosis is not known, but it is thought to be related to hormonal imbalances and immune system dysfunction.

Treatment for endometriosis may include medications to relieve pain and reduce inflammation, as well as hormonal therapies to reduce the growth of endometrial tissue. In some cases, surgery may be needed to remove the affected tissue.

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What was Darwin missing from his theory of natural selection and
how was it resolved?

Answers

Darwin was missing the mechanism of heredity from his theory of natural selection. This was resolved with the discovery of genetics and the understanding of DNA and genes as the carriers of hereditary information.

Darwin knew that traits were inherited, but he didn't know how exactly that process worked. This gap in his theory was later filled in by Gregor Mendel's work on pea plants and the development of the modern understanding of genetics.

Darwin's theory of natural selection was revolutionary and proposed that species evolved over time through a process of natural selection, whereby traits that were advantageous in a particular environment were more likely to be passed down to future generations.

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Surrounds nerve fibers, muscles, and fat cellsProvide mechanical support for the attached cellsGenerate signals that maintain cell survivalServe as a substratum for cell migrationSeparate adjacent tissues within an organ Act as a barrier to the passage of macromolecules. is called?

Answers

The structure that surrounds nerve fibers, muscles, and fat cells, provides mechanical support for the attached cells, generates signals that maintain cell survival, serves as a substratum for cell migration, separates adjacent tissues within an organ, and acts as a barrier to the passage of macromolecules is called the extracellular matrix (ECM).

The ECM is a complex network of proteins and carbohydrates that provides structural and biochemical support to the cells within a tissue. It is essential for tissue development, maintenance, and repair, and plays a crucial role in cell signaling and communication.
In addition to the functions you listed, the ECM also helps regulate cell behavior, influences cell differentiation and growth, and contributes to tissue repair and regeneration. The composition and structure of the ECM can vary depending on the tissue and organ it is found in, but it generally consists of a mix of fibrous proteins (such as collagen and elastin), glycoproteins, and proteoglycans.
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A conical flask containing 25 ml of peptone medium was inoculated with a total of 4x 10^6 cells of Escherichia
coli and incubated at 37°C with shaking to provide aeration. The stationary phase of 3 x 10^9 cells per ml was
reached after 284 minutes and no lag phase occurred.
What was the mean generation time to the nearest minute under these culture conditions?

Answers

The mean generation time (doubling time) of a bacterial culture is the time it takes for the number of cells in the culture to double. Thus the mean generation time of Escherichia coli in the peptone medium under these culture conditions is 99 minutes.

It can be calculated using the following formula:
Mean generation time = (t2 - t1) / (log N2 - log N1) Where t1 and t2 are the initial and final times, and N1 and N2 are the initial and final cell numbers. In this case, t1 = 0, t2 = 284 minutes, N1 = 4 x 10^6 cells, and N2 = 3 x 10^9 cells.
Plugging these values into the formula gives:
Mean generation time = (284 - 0) / (log 3 x 10^9 - log 4 x 10^6)
Mean generation time = 284 / (9.477 - 6.602)
Mean generation time = 284 / 2.875
Mean generation time = 98.78 minutes To the nearest minute, the mean generation time under these culture conditions is 99 minutes. Therefore, the mean generation time of Escherichia coli in the peptone medium under these culture conditions is 99 minutes.

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4) DESIGN A PMRALLE EXPERIMENT TO SIMULATE THE EFFECTS OF DRIFT. WHAT ARE THE EXPECTATIONS OF THAT EXPERIMENT?

Answers

A parallel experiment to simulate the effects of drift, the expectations of that experiment is the allele frequencies will fluctuate randomly over the course of the experiment

Drift, or genetic drift, is the random fluctuation of allele frequencies in a population due to chance events. This can lead to certain alleles becoming more or less common in a population over time. To simulate the effects of drift, we can use a simple experiment involving a bag of colored beads. Start with a bag of 100 beads, with 50 red beads and 50 blue beads, this represents our initial population with two different alleles (red and blue). Randomly draw 10 beads from the bag without looking, this simulates a random event that affects the population, such as a natural disaster.

Record the number of red and blue beads in the sample, this represents the new allele frequencies in the population after the random event. Replace the beads back into the bag and repeat steps 2 and 3 for a total of 10 rounds, this simulates the effects of drift over multiple generations. After 10 rounds, compare the final allele frequencies to the initial frequencies. You should see that the frequencies have changed due to the random events, demonstrating the effects of drift. So, the expectation of this experiment is that the allele frequencies will fluctuate randomly over the course of the experiment, and may end up being significantly different from the initial frequencies, this illustrates the concept of drift and how it can affect populations over time.

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Clade Excavata
The Excavates consist of a group of unicellular organisms that have modified mitochondria which undergo anaerobic respiration. Most have a body groove (hence excavation) which may act as a point of anchorage for their flagella. Examples include Giardia lambia, a human intestinal parasite transmitted by contaminated drinking water; Trychonympha sp., a mutualistic symbiont living in the gut of termites that aids in the digestion of wood; Euglena sp., a photosynthetic flagellate common in pond water; and Trypanosoma sp., a human blood parasite that causes African sleeping sickness.
Diplomonads
Figure 1. Giardia lambia is an intestinal parasite of vertebrates. It has a feeding stage called a trophozoite in its life cycle and a cyst stage. What you are looking at is the trophozoite. Note the two nuclei (that look like eyes) and the flagella (it has four to six although you might not see them all).
Euglenozoans
Euglena gracilis is referred to as a mixotroph -both heterotrophic and autotrophic.
Figure 2. Trypanosoma spp. are blood parasites with insect vectors. The circles in the image above are red blood cells. The protist has an long flagellum that runs its entire the length.
Question: What feature(s) do the excavates above share in common? Select all that apply.
1. Stigma (eye spot)
2. Multicellular
3. Heterotrophic
4. Photosynthetic
5. Flagellae
6. Parasitic
7. Motility
8. Cilia

Answers

The excavates above share the following features in common that includes Flagellae,Trychonympha ,Heterotrophic ,Parasitic ,Motility.


- Flagellae (5): All of the excavates mentioned, including Giardia lambia, Trychonympha sp., Euglena sp., and Trypanosoma sp., have flagella which they use for movement and/or anchorage.

- Heterotrophic (3): Giardia lambia, Trychonympha sp., and Trypanosoma sp. are all heterotrophic, meaning they obtain their energy from consuming other organisms.
- Parasitic (6): Giardia lambia and Trypanosoma sp. are both parasitic, meaning they live on or in a host organism and benefit at the host's expense.
- Motility (7): All of the excavates mentioned are capable of movement, either through the use of their flagella or other means.

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For a particular cell, the concentration of lactose is 32 mM on the inside of the cell and 0.65 mM on the outside, whereas the concentration of magnesium ions is 0.85 mM on the inside of the cell and 7.5 mM on the outside. The membrane potential is -135 mV, and the temperature is 28°C. What would be the net ΔG' for the coupling of two reactions: the inward transport of lactose and the inward transport of magnesium?

Answers

The net value of  ΔG' for the coupling of these two reactions is 8.5 kJ/mol.

The net ΔG' for the coupling of the inward transport of lactose and the inward transport of magnesium for a particular cell can be calculated as follows:

First, calculate the ΔG' of the lactose transport:
ΔG' of lactose transport = RT ln([lactose]in / [lactose]out)
= 8.314 x 28°C x ln(32 mM / 0.65 mM)
= 19.7 kJ/mol

Then, calculate the ΔG' of the magnesium transport:
ΔG' of magnesium transport = RT ln([magnesium]in / [magnesium]out)
= 8.314 x 28°C x ln(0.85 mM / 7.5 mM)
= -11.2 kJ/mol

Therefore, the net ΔG' for the coupling of these two reactions is 19.7 kJ/mol + (-11.2 kJ/mol) = 8.5 kJ/mol.

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Multiple choice
1. The 3 general type of R-Groups include
A) Charged, Acidic, Basic
B) Basic, Ion, Molecule
C) Ionic Covalent Acidic
2. Prions are:
A) infectious proteins
B) alternately folded proteins that are found in healthy organisms.
C) mad cow disease
D) all of the above
3. What are the three types of groups enzymes work with?
A) Co factors
B) Co enzymes
C) Prosthetic groups
D)All of the above

Answers

1. Charged, acidic, and basic R-Groups are the three main types of amino acids.

2. Infectious proteins called prions

3. Co factors, Co enzymes, and prosthetic groups are the three types of groups that enzymes interact with.

Amino acids can be broadly categorised into three classes: those with nonpolar R groups, those with uncharged polar R groups, and those with charged polar R groups.On the basis of the characteristics of the "R" group in each amino acid, amino acids may be divided into four main groupings.

The name "prions" refers to aberrant, pathogenic organisms that may spread and are capable of causing certain, normal cellular proteins known as prion proteins, which are most prevalent in the brain, to fold abnormally. These typical prion proteins' roles are yet not fully known.

Cofactors are essential elements of enzyme pathways that help regulate or activate enzymes. Metals like magnesium and copper as well as organic substances can serve as cofactors (e.g. heme, sugars, proteins). Cofactors, coenzymes, and prosthetic groups are the three different forms of cofactors.

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briefly describe what are the 5 fields of science that play a role in environmental assessment of land?

Answers

Answer: Chemistry,social science,social studies, and physics.

Explanation:

1. What is oxidative phosphorylation? 2. Describe the 3 stages in which food molecules are broken down. Explain what happens to the food we eat (proteins, carbohydrates and fats) that gives us energy.

Answers


Oxidative phosphorylation is a metabolic process that produces energy by transferring electrons between molecules. The three stages of food molecule breakdown are digestion, absorption, and metabolism.

1. In oxidative phosphorylation the electrons transfer energy to an electron acceptor, which is typically oxygen, and the energy is used to form ATP molecules. The ATP molecules are then used by cells to fuel metabolic processes.

2. During digestion, food molecules are broken down into smaller, more easily absorbed molecules, such as glucose and fatty acids. Absorption occurs in the small intestine, where molecules are taken into cells for further breakdown.

Finally, during metabolic processes, these molecules are broken down into simpler forms, such as carbon dioxide, water, and energy. Proteins are broken down into amino acids, carbohydrates into glucose, and fats into fatty acids and glycerol. The energy released from this process is used to fuel our cells and provide us with the energy we need to function.

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You observe a section of a cell in which there is much G actin
and no filaments of actin. How can this be?

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When we observe a section of a cell with a lot of G actin but no filaments of actin, it means that the G actin is not polymerized.

G actin is the monomeric form of actin, while F actin is the filamentous form of actin. In order for G actin to form F actin, it must undergo a process called polymerization. If there is no F actin present, it means that the polymerization process has not occurred.

This could be due to a lack of necessary proteins or ions that are required for the polymerization process to take place. It could also be due to the presence of proteins or molecules that inhibit the polymerization process.

In this case, there are no filaments present, likely because the G-actin molecules have not polymerized.

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How do you prepare 5 liters of fermentation brine in
pickling?
Why should cucumbers be desalted before pickling?
Compare the color, texture and shape

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To prepare 5 liters of fermentation brine for pickling, the following are needed: 5 liters of water, 5 tablespoons of salt, 2 tablespoons of sugar, and any other flavorings or seasonings that are suitable.

Cucumbers should be desalted before pickling to remove some of the natural bitterness from the cucumber and to prevent the cucumber from becoming too soft in the brine.

Desalting cucumbers also removes excess water, which can make pickles crunchier.

When comparing the color, texture, and shape of cucumbers before and after pickling, you will notice that pickled cucumbers are much brighter in color and have a slightly firmer texture than un-pickled cucumbers.

However, the shape of the cucumbers may remain the same, but pickled cucumbers tend to be slightly smaller due to the desalting process.

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happens best in solid materials.
A) conduction
b) Convection
c) Radiation

Answers

The answer is b) Conduction

How could Edgar separate the salt out of the salt-water mixture? A. use a magnet to attract the salt B. use chromatography C. evaporate off the water D. pour the mixture through a screen

Answers

Carefully remove the salt from the salt-water mixture and let the water evaporate.

What are the benefits of salt water?

Magnesium, zinc, iron, and potassium are just a few of the minerals abundant in saltwater. They can aid in the healing of any scrapes, cuts, or sores while also reducing inflammation and protecting our skin. In addition to enhancing lymphatic fluid flow, salt water also can help minimize the appearance or cellulite.

Is consuming saltwater healthy?

Due to its high salt content, salt water, especially ocean water, should not be consumed. The consumption of seawater can worsen dehydration because humans were designed to consume fresh water. It can seriously harm your body and increase your thirst if you consume salt water.

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1. Immunology Puzzle 1:
a. RFP, CEP, YFP,GFP b. GEP, YFP, REP, CEP c. BFP, CEP, YEP, RFP d. CFP, GFP, YFP, RFP d. BFP, GFP, YFP, REP 2. Immunology Puzzle 2:
a. IgA, IgG, IgM, IgD b. IgG, IgE, IgD, IgM, IgA c. IgE, IgA, IgD,IgM d. IgM, IgA, IgG, IgE e. IgA, IgM, IgD, IgG, IgE 3. Immunology Puzzle 3: a. TLR3 - TLR4 - TLR1/TLR2/TLR6 - TLRS/TLR7/8/TLR9/TRL11 b. TLR1/TLR2/TLR6 - TLR3 TLR5/TLR7/8/TLR9/TRL11 - TLR4 c. TLR5/TLR7/8/TLR9/TRL11 - TLR4 - TLR1/TLR2/TLR6 - TLR3

Answers

Immunology Puzzle 1:
a. RFP, CEP, YFP,GFP
b. GEP, YFP, REP, CEP
c. BFP, CEP, YEP, RFP
d. CFP, GFP, YFP, RFP
e. BFP, GFP, YFP, REP


Immunology Puzzle 2:
a. IgA, IgG, IgM, IgD
b. IgG, IgE, IgD, IgM, IgA
c. IgE, IgA, IgD,IgM
d. IgM, IgA, IgG, IgE
e. IgA, IgM, IgD, IgG, IgE


Immunology Puzzle 3:
a. TLR3 - TLR4 - TLR1/TLR2/TLR6 - TLRS/TLR7/8/TLR9/TRL11
b. TLR1/TLR2/TLR6 - TLR3 TLR5/TLR7/8/TLR9/TRL11 - TLR4
c. TLR5/TLR7/8/TLR9/TRL11 - TLR4 - TLR1/TLR2/TLR6 - TLR3



1. Immunology Puzzle 1: The correct answer is option d. CFP, GFP, YFP, RFP. These are all fluorescent proteins that are commonly used in immunology research.

2. Immunology Puzzle 2: The correct answer is option b. IgG, IgE, IgD, IgM, IgA. These are all types of immunoglobulins, which are proteins that play a crucial role in the immune system.

3. Immunology Puzzle 3: The correct answer is option a. TLR3 - TLR4 - TLR1/TLR2/TLR6 - TLRS/TLR7/8/TLR9/TRL11. These are all types of Toll-like receptors, which are proteins that play a crucial role in the innate immune system.

In conclusion, the correct answers for the Immunology Puzzles are option d for Puzzle 1, option b for Puzzle 2, and option a for Puzzle 3.

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Choose the correct statement about lysosome and peroxisome functions:
1)Lysosomes and peroxisomes both break down wastes and/or unwanted substances, including long-chain fatty acids.
2)Lysosomes and peroxisomes both break down wastes and/or unwanted substances, but lysosomes also start long-chain fatty acid hydrolysis for respiration.
3)Lysosomes and peroxisomes both break down wastes and unwanted substances, but peroxisomes also store glycogen.
4)Lysosomes and peroxisomes both break down wastes and/or unwanted substances, but peroxisomes also start long-chain fatty acid hydrolysis for respiration.
5)Lysosomes break down wastes and/or unwanted substances, and peroxisomes synthesize long-chain fatty acids.

Answers

The correct statement about lysosome and peroxisome functions is: 1) Lysosomes and peroxisomes both break down wastes and/or unwanted substances, including long-chain fatty acids.

Lysosomes are membrane-bound organelles that contain hydrolytic enzymes that are used to break down a variety of substances, including long-chain fatty acids. Peroxisomes are also membrane-bound organelles that contain enzymes that are used to break down a variety of substances, including long-chain fatty acids.

However, peroxisomes also contain enzymes that are involved in the synthesis of certain lipids and the detoxification of harmful substances. Therefore, while both lysosomes and peroxisomes are involved in the breakdown of wastes and/or unwanted substances, they also have distinct functions.

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