The amount of a specific protein in a cell is influenced by different factors. Which are the following are likely to play a role in determining the amount of a specific protein present in a cell? Select ALL that apply.
Multiple answers: Multiple answers are accepted for this question
The transport of the mRNA for the specific protein from the nucleus to the cytosol.
The processing of the primary RNA for the specific protein.
The rate of transcription of the gene for the specific protein.
The half-life of the specific protein in the cytosol.

Answers

Answer 1

All of the options listed are likely to play a role in determining the amount of a specific protein present in a cell.

The transport of the mRNA for the specific protein from the nucleus to the cytosol is important because it determines how much of the mRNA is available for translation into protein in the cytosol.

The processing of the primary RNA for the specific protein is also important because it determines how much of the RNA is available for translation into protein. Processing includes steps such as splicing, which removes introns and joins exons together, and the addition of a 5' cap and a 3' poly-A tail, which help protect the RNA from degradation and promote translation.

The rate of transcription of the gene for the specific protein is important because it determines how much mRNA is produced in the first place. The more mRNA that is produced, the more protein can potentially be made.

The half-life of the specific protein in the cytosol is important because it determines how long the protein will be present in the cell before it is degraded. The longer the half-life, the more protein will be present in the cell at any given time.

Overall, all of these factors play a role in determining the amount of a specific protein present in a cell.

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Related Questions

A case study from Scientific American Journal,
A French woman was slim and married. She spent more than 5-6 years hoping to have a baby but never did. The husband consulted with a doctor. The doctor examined her body and physiology. He gave a prescription, but it was not medication. Instead, he advised her to take a glass of red wine and a piece of roasted chicken for three to four months. She was surprised that she could expect a baby after five to six months. He knew that this was what was going to happen.
What could be the reason why the doctor prescribed that? How and why did she get pregnant after this unusual prescription?

Answers

The reason why the doctor prescribed a glass of red wine and a piece of roasted chicken for the French woman is most likely because she was suffering from a nutrient deficiency.

It is possible that she was not getting enough of the essential nutrients needed for a healthy pregnancy, such as iron, folic acid, and protein.

Red wine is a good source of antioxidants, which can help to protect the body from oxidative stress and inflammation. Roasted chicken is a good source of protein, which is essential for the growth and development of the fetus.

After following the doctor's prescription for three to four months, the French woman was able to get pregnant because her body was now receiving the essential nutrients needed for a healthy pregnancy.

The red wine and roasted chicken provided her with the antioxidants, protein, and other essential nutrients that her body needed in order to support a healthy pregnancy.

As a result, she was able to conceive and carry a baby to term. This case study shows the importance of good nutrition and the role that it plays in fertility and pregnancy.

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5. The radiation balance on Earth is governed primarily by two main cycles of
heating and cooling. (5 points)
A. What is the name of the daily cycle? (2 points)
B. What happens during this cycle? (3 points)

Answers

A. The name of the daily cycle is the diurnal cycle.

What is Diurnal cycle?

During the diurnal cycle, the Earth's surface is heated by the sun during the day, causing the surface to warm up and radiate heat. At night, when the sun is no longer present, the Earth's surface cools down and radiates heat out into space. This cycle of heating and cooling is essential for regulating temperatures on Earth and maintaining a balance in the radiation budget.

The diurnal cycle, also known as the daily cycle, refers to the pattern of environmental changes that occur over a 24-hour period, such as changes in temperature, light, and atmospheric pressure. These changes are driven by the rotation of the Earth on its axis, resulting in a cycle of heating and cooling, as well as changes in other environmental factors. The diurnal cycle has a significant impact on many aspects of life on Earth, including plant growth and animal behavior, and is an important factor to consider in many scientific fields, such as meteorology, ecology, and physiology.

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8. In human males, which structure is used to transport sperm out of the body?
a. Oviduct
c. Ureter
b. Scrotum
d. Urethra

Answers

Answer:d.Urethra

Explanation:

which does not belong? (there can be teo answers gor this one. comeup eitj both answers. think functional vs structural.

Answers

Functionally, a tissue does not belong because it is a structural component of the body and Structurally, an organ does not belong because a tissue is composed of multiple cells that are organized to carry out specific functions.

What is functional perspective?

Functional perspective is an approach to understanding social behavior by looking at how different parts of a system interact to produce a whole. It focuses on the functions that different aspects of society play in maintaining social order.

Functionally, the answer could be "structure" because it does not directly carry out any of the tasks or functions that the other items in the list do. Structurally, the answer could be "algorithm" because it does not have the physical characteristics that the other items have.

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2. Briefly describe two physical properties of rock that are of
primary interest to environmental practice
and the underground storage and flow of water.

Answers

Physical properties of rock that are important to environmental practice and the underground storage and flow of water include permeability and porosity.

We proceed to analyze the two physical properties that the rock must have:

Porosity: The degree to which a rock can hold water is referred to as its porosity. Porosity is defined as the ratio of the volume of pore spaces to the volume of the rock sample. The amount of water that can be stored in a rock is determined by its porosity. Permeability: The capacity of rock to allow water to flow through it is referred to as permeability. It's a function of the number and size of the pore spaces in the rock, as well as the degree to which they're connected. Permeability is determined by the rock's structure and composition.

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choose a microorganism, describe that organism. any virulence
factors, maybe how it looks under the scope, how its treated, how
its transmitted?

Answers

The microorganism I will choose is the bacterium Staphylococcus aureus. It is a Gram-positive coccus-shaped bacterium found in the human nasal cavity and on the skin. Its virulence factors include the production of toxins such as leukocidin, protease, lipase, and hemolysins. It can be treated with antibiotics such as penicillin, cephalosporins, and vancomycin. Transmission is through contact with infected people or surfaces. Under the microscope, it appears as round or slightly ovoid, occurring in grape-like clusters.

The microorganism I have chosen is Staphylococcus aureus, a Gram-positive bacterium. It is often found on the skin or in the nose of humans, and it is associated with many different types of infections, from mild skin and respiratory infections to more serious ones such as septicemia and endocarditis.

S. aureus is a virulent organism, with many virulence factors including surface proteins, capsules, and pili, which can facilitate adhesion to host cells.

Under the microscope, S. aureus appears as spherical, gram-positive cocci, arranged in clusters or grape-like arrangements. Treatments for infections caused by S. aureus can include antibiotics, such as penicillin, and in some cases, antiviral drugs.

S. aureus is usually spread by contact with infected individuals, and can be spread from person to person through contact with skin lesions, or through contact with contaminated objects.

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What are the ocular and objective lenses in a compound microscope? What are the four objectives typically found on a teaching microscope like the ones we use?
How is the pointer/ocular micrometer used to estimate the dimensions of an object being viewed under the microscope?

Answers

The ocular lens, also known as the eyepiece, is the lens closest to your eye when looking into the microscope. It magnifies the image of the object being viewed. The objective lens is the lens closest to the specimen and it provides the initial magnification.

There are typically four objectives found on a teaching microscope. These are: 4x, 10x, 40x, and 100x. The 4x provides the lowest magnification, while the 100x provides the highest magnification.

The pointer/ocular micrometer is used to estimate the dimensions of an object being viewed under the microscope. It works by having two or more sets of cross-hairs or lines on the ocular lens, with each set being a known distance apart. By measuring the number of sets that the object spans, the approximate size of the object can be determined.

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How many grams sodium formate (HCOONa), 68.0069 g/mol) do you need to add to 500 ml of 0.50 M formic acid (HCOONa) for a pH 3 buffer. Ka = 1.77 x 10-4

Answers

We need to add 5.95 grams of sodium formate (HCOONa) to 500 ml of 0.50 M formic acid (HCOOH) for a pH 3 buffer.

To calculate the amount of sodium formate (HCOONa) needed to add to 500 ml of 0.50 M formic acid (HCOOH) for a pH 3 buffer, we can use the Henderson-Hasselbalch equation:

pH = pKa + log([A-]/[HA])

Where pH is the desired pH, pKa is the acid dissociation constant, [A-] is the concentration of the conjugate base (sodium formate), and [HA] is the concentration of the weak acid (formic acid).

Rearranging the equation to solve for [A-]:

[A-] = [HA] x 10^(pH - pKa)

Substituting the given values:

[A-] = 0.50 M x 10^(3 - (-log(1.77 x 10^-4)))

[A-] = 0.50 M x 10^(3 - 3.752)

[A-] = 0.50 M x 10^(-0.752)

[A-] = 0.175 M

To convert from molarity to grams, we can use the formula:

grams = molarity x volume x molar mass

Substituting the given values:

grams = 0.175 M x 0.500 L x 68.0069 g/mol

grams = 5.95 g

Therefore, we need to add 5.95 grams of sodium formate (HCOONa) to 500 ml of 0.50 M formic acid (HCOOH) for a pH 3 buffer.

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_______ a bone with the following descriptive forms or landmarked: greater and lesser tubercles, a trochlea and a capitulum, and a head with an intertubercular groove.

Answers

The bone that fits the description provided is the humerus bone. The humerus bone is the long bone in the upper arm that connects the shoulder to the elbow.

The greater and lesser tubercles are located at the proximal end of the humerus bone, and they serve as attachment points for muscles. The trochlea and capitulum are located at the distal end of the humerus bone, and they form part of the elbow joint. The head of the humerus bone is located at the proximal end, and it fits into the glenoid cavity of the scapula to form the shoulder joint. The intertubercular groove, also known as the bicipital groove, is located between the greater and lesser tubercles, and it serves as a pathway for the tendon of the biceps muscle.

In conclusion, the bone that has the greater and lesser tubercles, a trochlea and a capitulum, and a head with an intertubercular groove is the humerus bone.

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Imagine that the aminoacyl-tRNA synthase that normally attaches leucine to its cognate tRNAs is mutated such that it attaches other amino acids, in addition to leucine, to leucine-specific tRNAs. What effect will this mutation have on the cell (i.e., what will be the phenotype)? Be as specific as possible.

Answers

The mutation in the aminoacyl-tRNA synthase that attaches other amino acids, in addition to leucine, to leucine-specific tRNAs will have a detrimental effect on the cell. This is because the tRNA will now carry the wrong amino acid, leading to the incorporation of incorrect amino acids into the polypeptide chain during protein synthesis. As a result, the protein structure and function will be altered, leading to a variety of potential phenotypes, including loss of protein function, protein misfolding, and cellular toxicity.

Furthermore, this mutation can also lead to the production of truncated proteins, as the incorporation of incorrect amino acids can lead to the formation of premature stop codons. This will result in the production of non-functional proteins, which can have a negative impact on the cell's overall function and viability.

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What must be true of the F1 ​generation that came from Mendel's cross (breeding experiment) of two phenotypically different plants of thePgeneration? A.F1 plants are sterile B.F1 plants are heterozyogous C.F1 plants are homozygous D.F1 plants are true-breeding

Answers

The F1 ​generation that came from Mendel's cross (breeding experiment) of two phenotypically different plants of the P generation are heterozygous F1 plants. The correct answer is B. F1 plants are heterozygous.

Mendel's cross of two phenotypically different plants of the P generation resulted in the F1 generation, which are all heterozygous. This means that they have two different alleles for a particular trait, one from each parent. In Mendel's experiment, he crossed a true-breeding plant with purple flowers (PP) with a true-breeding plant with white flowers (pp). The F1 generation all had purple flowers (Pp), indicating that they were heterozygous for the flower color trait.

A. F1 plants are sterile - This is not true, as the F1 generation is able to reproduce and create the F2 generation.

C. F1 plants are homozygous - This is not true, as the F1 generation is heterozygous, with two different alleles for a particular trait.

D. F1 plants are true-breeding - This is not true, as the F1 generation is heterozygous and will not always produce offspring with the same phenotype.

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An introduced species is best described as a species that
• A. increases biodiversity
• B. does not normally live in an area
• c. has no permanent home
• D. is a poor predator

Answers

Answer:

does not have a permanent home

(SATA) The ff are the functions of the Heart: a. pumps blood throughout the body
b. delivers oxygen- and nutrient-rich blood totissues and organs
c. removes the carbon dioxide and waste products of meta

Answers

(SATA) The ff are the functions of the Heart:

a. pumps blood throughout the body

b. delivers oxygen- and nutrient-rich blood totissues and organs

c. removes the carbon dioxide and waste products of meta

Correct answer: a, b, c

The heart performs several important functions in the body, including:
a. Pumping blood throughout the body: The heart is responsible for circulating blood throughout the body in order to deliver oxygen and nutrients to the tissues and organs.
b. Delivering oxygen- and nutrient-rich blood to tissues and organs: The heart pumps oxygen- and nutrient-rich blood to the tissues and organs in order to support their functions.
c. Removing carbon dioxide and waste products of metabolism: The heart also plays a role in removing carbon dioxide and other waste products of metabolism from the body.
The heart is a crucial organ that plays a vital role in maintaining the health and functioning of the body.

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Only
nine species of existing land mammals grow to adu: body weights
over 1000 kg (1 megagram). All are herbivores bod employ
fermentative digestion. These "megaherbirdie that he two species of
elepha

Answers

The nine species of land mammals that can grow to a body weight of over 1000 kg are herbivores that use fermentative digestion. These "megaherbivores" include two species of elephants, five species of rhinos, the common hippo, and the giraffe.

The metabolic pros of such large size are the capacity for the body to more efficiently regulate its internal temperature, the capacity to feed on plants that are inedible to smaller animals, and the capacity to digest large amounts of vegetation. The metabolic cons of such large size include a slower metabolism and the need for a much larger energy intake to sustain the large body size.

No terrestrial carnivores achieve such large size because their predatory lifestyle necessitates agility and speed which would be compromised by large size. Large carnivores would also require a larger territory to sustain their food requirements and would therefore be more vulnerable to predation.

Here's the full task:

Only nine species of existing land mammals grow to adu: body weights over 1000 kg (1 megagram). All are herbivores bod employ fermentative digestion.

These "megaherbirdie that he two species of elephants, the five species of rhinos it. common hippo, and the giraffe.

What are the metabolic pros con cons of such large size? Can you suggest why no terress carnivores achieve such large size?

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the types of resources you think whales used in ancient bodies of water

Answers

RESOURCES WHALES USED IN BODIES OF WATERFOODSHELTERNAVIGATIONBREEDING GROUNDSMIGRATION ROUTES

Recent discoveries of microscopic fossils have extended the known history of life to about: Multiple Choice a) 2 billion years ago. b) 3.5 billion years ago. c) 1 billion years ago. d) 45 billion years ago.

Answers

Recent discoveries of microscopic fossils have extended the known history of life to about b) 3.5 billion years ago.

Recent discoveries of microscopic fossils, such as stromatolites and microfossils of prokaryotes, have pushed back the known history of life on Earth to around 3.5 billion years ago. These fossils provide evidence for the existence of simple, single-celled organisms that lived in shallow waters and were capable of photosynthesis. Prior to these discoveries, the oldest known fossils were about 2.1 billion years old. The study of these ancient organisms is important for understanding the evolution of life on Earth and the conditions that led to the emergence of more complex organisms. Additionally, it has implications for the search for extraterrestrial life, as it suggests that life may be more common in the universe than previously thought.

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Assume that the long septum of the nose is autosomal dominant and exhibits 20% penetrance. A person who is heterozygous with a long septum crosses with a person who is homozygous and has a normal septum. What is the probability that they will have a child with a long septum?

Answers

The probability of having a child with a long septum in this case is 10%.

To determine this, we can use a Punnett square to find the probability of each possible genotype for the offspring. Since the long septum trait is autosomal dominant, we will use the letter L to represent the dominant allele and l to represent the recessive allele.

The heterozygous parent has the genotype Ll, while the homozygous parent has the genotype ll.

 | L | l
--|---|--
l | Ll | ll
l | Ll | ll

From the Punnett square, we can see that there is a 50% chance of the offspring having the genotype Ll (heterozygous) and a 50% chance of the offspring having the genotype ll (homozygous recessive).

However, since the long septum trait exhibits 20% penetrance, only 20% of individuals with the dominant allele will actually express the trait. Therefore, the probability of having a child with a long septum is 50% (probability of having the dominant allele) x 20% (probability of expressing the trait) = 10%.

So the probability of having a child with a long septum in this case is 10%.

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What evidence supports the claim the H allele is dominant and the h allele is recessive? PLEASE HELP

Answers

the inheritance patterns, biochemical studies, and population genetics all support the idea that the H allele is dominant over the h allele in the ABO blood group system.

The H allele and h allele refer to alleles of the ABO blood group system, which is controlled by a single gene on chromosome 9. The ABO gene codes for an enzyme that attaches specific sugars to red blood cell surfaces. The H allele codes for an enzyme that adds a specific sugar called fucose to the red blood cell surface, while the A and B alleles code for enzymes that add different sugars to the red blood cell surface.

Some of the evidence supporting the dominance of the H allele includes:

inheritance patterns: The ABO blood group system follows classic Mendelian inheritance patterns, with the H allele being dominant over the h allele.

Biochemical studies: Biochemical studies have shown that individuals who are heterozygous (Hh) or homozygous (HH) .

Population genetics: Studies of the distribution of ABO blood groups in different populations have shown that the H allele is much more common than the h allele.

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When did Staphylococcus aureus become resistant to penicillin?

Answers

Staphylococcus aureus became resistant to penicillin in the early 1940s.

This was just a few years after penicillin was first introduced as a treatment for bacterial infections. The resistance occurred due to the production of the enzyme penicillinase, which breaks down the penicillin molecule and renders it ineffective. This has led to the development of other antibiotics, such as methicillin, to treat Staphylococcus aureus infections. However, resistance to these antibiotics has also developed over time.

Today, Staphylococcus aureus strains that are resistant to multiple antibiotics are commonly referred to as methicillin-resistant Staphylococcus aureus (MRSA). MRSA infections can be difficult to treat and are a significant cause of hospital-acquired infections. The continued emergence of antibiotic-resistant bacteria highlights the importance of responsible antibiotic use and the development of new treatment strategies.

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Please help


What is the phenotype of a male who is heterozygous for the widow's peak?

Answers

Answer:

The phenotype of a male who is heterozygous for the widow's peak will have the dominant trait, which is the widow's peak.

Explanation:

Two new microorganisms, labeled X and Y, were isolated from an oil spill during a search for organisms that will degrade petroleum products. DNA was isolated from each, and the percent adenine was measured. Assume each organism contains normal double-stranded DNA.
Organism X: 18.7% adenine
Organism Y: 29.9% adenine
a.) What percentage of thymine does organism X have?
b.) What percentage of cytosine does organism Y have?
c.) Which organism will have the higher melting DNA (require a higher temperature to denature)?

Answers

a.) Organism X has 18.7% thymine.
b.) Organism Y has 20.1% cytosine.
c.) Organism Y will have the higher melting DNA.

a.) Organism X will have 18.7% thymine because adenine and thymine always pair together in DNA, and they will always have the same percentage.
b.) Organism Y will have 20.1% cytosine because adenine and thymine make up 59.8% of the DNA (29.9% + 29.9%), and the remaining 40.2% will be split equally between cytosine and guanine. So, 40.2% / 2 = 20.1% cytosine.
c.) Organism Y will have the higher melting DNA because it has a higher percentage of adenine and thymine, which form two hydrogen bonds, compared to cytosine and guanine, which form three hydrogen bonds. The more hydrogen bonds, the higher the melting temperature required to denature the DNA.

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Explain the structure of a protein
How do you manage to activate and deactivate?
Using 2 examples, explain, how does it works from a
chemical point of view?

Answers

Proteins are large, complex molecules made up of amino acids. The structure of a protein is determined by its primary structure, which is the sequence of amino acids. This primary structure can be further folded into secondary, tertiary and quaternary structures.

Activation and deactivation of proteins can be done by several methods, including changes in pH, binding of small molecules, or through post-translational modifications.

For example, protein kinases are enzymes that can phosphorylate other proteins and thus activate them. Similarly, phosphatases can dephosphorylate proteins and thereby inactivate them.

In another example, covalent modifications can modify the activity of a protein. Ubiquitination is a process where ubiquitin molecules bind to lysine residues in proteins and inactivate them. Alternatively, deubiquitination can activate the protein.

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You find this raised white plaque on a feline exam. You do a cytology and find high numbers of eosinophils & mast cells. You suspect…

Answers

I suspect that  the presence of an allergic or inflammatory condition. Additional testing, such as bloodwork and/or skin tests, may be necessary to diagnose the underlying cause of the elevated eosinophils and mast cells.

the feline may have an eosinophilic plaque. Eosinophilic plaques are a common skin condition in cats that are characterized by raised, white, ulcerated lesions that are typically found on the abdomen, thighs, or near the anus. They are often associated with high numbers of eosinophils and mast cells on cytology, which are immune cells that play a role in allergic reactions and inflammation. Eosinophilic plaques are often itchy and can be caused by a variety of factors, including allergies, parasites, or immune-mediated diseases. Treatment typically involves identifying and addressing the underlying cause, as well as providing supportive care for the skin lesions.

Atypical Langerhans cell growth characterises human eosinophilic granuloma (LCs). Dendritic cells give rise to LCs, which are antigen-presenting cells. Eosinophilic granulomas are benign tumours that primarily affect children and adolescents in humans. EG is a somewhat uncommon illness that affects slightly more men than women overall and more white people than black people. 4-5 children (under 15) per million per year and 1–2 adults per million per year develop EG.

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"Hydrostatic pressure in the Bowman’s capsule can drastically
increase by ________________.
Group of answer choices"
a) decrease
b) increase
c) remain the same

Answers

The hydrostatic pressure in Bowman's capsule can drastically increase by an B) increase in blood pressure.

The Bowman's capsule is a part of the nephron, which is responsible for filtering blood and forming urine. The pressure in the glomerular capillaries, which are located in the Bowman's capsule, is essential for the filtration process.

An increase in blood pressure causes an increase in hydrostatic pressure, which can lead to increased filtration rate and increased urine formation. On the other hand, a decrease in blood pressure can cause a decrease in hydrostatic pressure, leading to decreased filtration and decreased urine formation.

Therefore, the hydrostatic pressure in Bowman's capsule is dependent on blood pressure, and any changes in blood pressure can have a significant impact on filtration and urine formation.

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When a plant needs to conserve water, the guard cells of the
stomata will be
Enlarged
Shrunken
Water conservation has no effect on guard cells

Answers

When a plant needs to conserve water, the guard cells of the stomata will be shrunken.

Guard cells are specialized cells that surround the stomata, small openings on the surface of plant leaves. When the guard cells are full of water, they become enlarged and the stomata open, allowing for gas exchange and water loss through transpiration. However, when the plant needs to conserve water, the guard cells lose water and become shrunken, causing the stomata to close and reducing water loss. This is an important mechanism for plants to regulate their water usage and prevent excessive water loss in dry conditions.

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Calcium regulation in the ER is based on the use of 2 different proteins to maintain a stock of calcium so the cell can use it when necessary. A aigand gated calcium channel and a Calcium ATPase. Which sentence best describes how these channels are used to regulate calcium levels in the cytosol(where is active) vs when it is in the ER (in a storage unit)
a. The ligand gated calcium channel and the calcium ATPase are both used interchangeably
b. A ligan gated calcium channel opens to let calcium when the cell requents (ligand) and the calcium ATPase to shove the calcium back in
c. All of these answer are correct

Answers

The best description used to regulate calcium levels vs ER is -  A ligand gated calcium channel opens to let calcium when the cell requents (ligand) and the calcium ATPase to shove the calcium back in."  Therefore the correct option is option B.

Calcium regulation in the ER is based on the use of two different proteins, a ligand gated calcium channel and a Calcium ATPase. The ligand gated calcium channel is responsible for allowing calcium to enter the cytosol when it is needed by the cell.

The Calcium ATPase, on the other hand, is responsible for moving calcium back into the ER when it is no longer needed in the cytosol. By using these two proteins in conjunction, the cell is able to maintain a stock of calcium and use it when necessary.

Therefore the correct option is option B.

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3a.You will make 10ml of 1mg/ml (or 1000g /ml) of BSA. How much will you weigh (in GRAMS) for 10mL of 1mg/ml? ____________
3b. Second, you will make 10ml of 200g/ml. (Hint: Use C1V1=C2V2) How much 1000g /ml BSA will you add to make 10mL of 200g/ml? _____________ How much water will you add to this solution? ________________

Answers

3a. To make 10mL of 1mg/mL of BSA, you will need to weigh out 0.01 grams of BSA.

3b. you will need to add 2mL of the 1000µg/mL BSA stock solution to make 10mL of 200µg/mL and you will need to add 8mL of water to the solution.

To make 10mL of 1mg/mL of BSA, you will need to weigh out 0.01 grams of BSA. This is because 1mg/mL is equivalent to 0.001g/mL, and multiplying this by 10mL gives you 0.01 grams.
To make 10mL of 200µg/mL from a stock solution of 1000µg/mL, you can use the equation C1V1=C2V2.

Plugging in the values gives you (1000µg/mL)(V1) = (200µg/mL)(10mL). Solving for V1 gives you V1 = 2mL. This means you will need to add 2mL of the 1000µg/mL BSA stock solution to make 10mL of 200µg/mL. To find out how much water you will need to add, you can subtract the volume of the stock solution from the final volume: 10mL - 2mL = 8mL. So you will need to add 8mL of water to the 2mL of stock solution to make 10mL of 200µg/mL BSA.

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The
old microscopes do not allow a visibility of the cells because they
had the limitation of:
a. magnification
b. refraction
c. resolution
d. number of nuclei
e. both A and C

Answers

The old microscopes do not allow a visibility of the cells because they had the limitation of both magnification and resolution. Therefore, the correct answer is option e. both A and C.

Magnification refers to the ability of a microscope to enlarge an image, while resolution refers to the ability of a microscope to distinguish between two separate points. Both of these factors are important in being able to see cells clearly. Older microscopes had lower magnification and resolution capabilities, making it more difficult to see cells.

it is crucial to highlight that the fundamental limiting factor for sight of cells in previous microscopes was resolution, not merely magnification. The resolution of traditional microscopes is restricted by the wavelength of visible light, which makes it impossible to examine features smaller than the limit of the wavelength. Consequently, even with high magnification, the features within cells could not be resolved with previous microscopes due to their poor resolution. In contrast, contemporary microscopes, such as electron microscopes, have far better magnification and resolution capabilities, which allow for much clearer and detailed views of cells.

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2. Colloidal particles in an untreated suspension usually have a mix of electrostatic charges making them tend to stick together and easy to filter. True or False?
3. A 240 m section of newly installed 205 mm diameter water main is pressure tested for leakage. It was observed that 12 L of water was pumped into the pipeline to maintain the required pressure of 1000 kPa. The pipe sections are 6 m long between joints. Has the allowable rate of leakage been exceeded?

Answers

The given statement that colloidal particles in an untreated suspension usually have a mix of electrostatic charges making them tend to stick together and easy to filter  is true. The allowable rate of leakage has not been exceeded.

Colloidal particles in an untreated suspension typically have a mix of electrostatic charges that cause them to stick together and become easy to filter.

The allowable rate of leakage is 0.6 L/m/h. To calculate the leakage rate, the volume of water pumped in needs to be divided by the length of the section of the pipeline (240 m) and the pressure testing duration (1 hour). Therefore, the leakage rate is as follows:

Volume of water pumped in = 12 LSection of pipeline = 240 mDuration of pressure testing = 1 hourLeakage rate = Volume of water pumped in / Section of pipeline / Duration of pressure testing= 12 / 240 / 1= 0.05 L/m/h

Since the leakage rate is less than the allowable rate of 0.6 L/m/h, the allowable rate of leakage has not been exceeded. Therefore, the pipeline has passed the pressure test.

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Part B: Presumptive Test for Coliforms
1. Weigh out 35grams of McConkey broth powder and add to 1 litre of water in a conical flask. Shake and invert the conical flask to ensure the solution is well mixed.
2. Set up test-tube and a put Durham tube upside down into every test-tube.
3. Using pipettes transfer 10 ml of the McConkey broth into each test tube.
4. Put a suitable cap onto the test tubes and then cover them tightly with tinfoil.
5. Place all of the test tubes into an autoclave.
6. Remove the test tubes form the autoclave and label 4 test tubes with area sampled and 1ml, 2ml, 3ml and 5ml
7. Using a sterile pipette transfer 1 ml of the water sample into the test tube labelled 1ml, 2ml of the water sample into the test tube labelled 2ml, 3ml of the water sample into the test tube labelled 3ml and 5ml of the water sample into the test tube labelled 5ml.
8. Cover the test tubes with the caps and place them into the incubator for 48 hours at 37oC.
9. If the colour changes from purple to a white-yellowish colour and the Durham test tube is translucent the result is positive. Therefore, there are E-coli organism present in the water.
Questions:
1. What is the principle underpinning this experimental procedure?
2. What were the major findings? The conclusion could provide a brief explanation of what the final data from the experiment indicates.
3. What were the errors or possible errors. Could this experiment be improved in future?
4. Discuss the significance of the experiment. Where is the experiment used? What is this experiment used for? What are the practical applications?

Answers

The principle underpinning this experimental procedure is the presumptive test for coliforms.

This is a type of biochemical test used to detect coliform bacteria in water, which can indicate the presence of fecal contamination. The test utilizes a culture medium (McConkey broth) to differentiate coliforms from other gram-negative bacteria. The test is based on the fermentation of lactose, which leads to the production of gas (carbon dioxide) in the Durham tube if coliforms are present.

The major findings of this experiment were that if the colour of the medium changed from purple to a white-yellowish colour and the Durham test tube was translucent, the result was positive, indicating the presence of E. coli organisms in the water.

Possible errors in this experiment include incorrect volume measurements when transferring the sample into the test tubes, contamination from inadequate sterilization of the test tubes, and incorrect incubation temperatures. This experiment could be improved in future by taking extra precaution to ensure proper sterilization and by using digital pipettes for more accurate volume measurements.

The significance of this experiment lies in its ability to detect the presence of fecal contamination in water. This experiment is used to monitor the quality of water in various settings, such as public water systems, and can help to ensure that the water is safe for consumption. The practical applications of this experiment include testing the safety of drinking water and wastewater before it is released into the environment.

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