What restriction enzyme would you use to cut a plasmid for insertion of the gene of interest in the creation of a recombinant plasmid?

Answers

Answer 1

Answer:

Restriction endonucleases

Explanation:

Restriction endonucleases are restriction enzymes that is used in cutting of deoxyrbonucliec acid (DNA)that recognizes a target sequence known as target site and then cuts the DNA into two pieces at or near that site.

Many restriction enzymes produce cut the ends with short, single strand, two of which if match can base pair and stick together. The enzyme DNA ligase helps in the joining of the molecule which seals gaps in the DNA backbone.


Related Questions

PLEASE I NEED THIS I WILL MARK BRAINLIEST AND GIVE YOU 50 POINTS

Answers

Answer:

Du kannst ich bekomme nur die Kerze und ich werde dir die Kerze besorgen und ich spiele sein sein und und werde

Help please? 5 part punnett square bio assignment. 40 POINTS! oh and its due in an hour :P

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why did you delete mt answer. also i did it for you just dont know how to send it

Question 4 (1 point) What vocabulary term is used when looking at the ratio for the genetic makeup of an organism?​

Answers

The answer would be: genotype
It is called the genotype

Which of these is a ball and stick model?​

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i’m pretty sure it’s the third one or the furthest on the right
Most likely the third one if not I am sorry

the temperate continental region is regulated by air masses moving over the ocean. true or false

Answers

Answer:

False

Explanation:

Which effect of pollution does the photograph show?

Answers

It would be Eutrophication

Answer:

C. Eutrophication

Explanation:

Is this correct? Brainliest involved a

Answers

Answer:

the answer B

Explanation:

1. What parts of the world are expected to see the largest increase in people moving from rural (farming areas) to urban (city) areas?
NEED ASAP
I WILL BUT BRAINLIEST

Answers

Answer:

South

Explanation:

In the south

South in the south I hope this helped

What is common to all cells?

Answers

Explanation:

Parts common to all cells are the plasma membrane, the cytoplasm, ribosomes, and genetic material.

The plasma membrane, cytoplasm, DNA, and ribosomes make up all cells. 

What is a cell?

Every living thing is made of smaller and smaller components called cells. There are trillions upon trillions of cells that make up the human body. They take in nutrients from meals, transform those nutrients into energy, and carry out specialized functions while doing so. They are responsible for giving structure for the body.

All cells have four parts:

A plasma membrane, which divides the inside of the cell from its surroundings; Cytoplasm, which is a jelly-like region within the cell that contains various biological components; DNA, which is the genetic material of the cell; and Ribosomes, the protein factory.

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33 points
Animals are heterotrophic, meaning that they participate in food chains
and get energy from consuming organic compounds. Which other
biological kingdom is made up entirely of heterotrophic organisms? *
bacteria
O fungi
O plants
O protists
cameraniem
......

Answers

Answer:

Plants!

Explanation:

Plants are the only other heterotrophs in all the kingdoms.

Hope this helps!

Answer:

protist

Explanation:

thank you francesca81

NEED HELP ASAP 15 POINTS
what does it mean, Genotypically and Phenotypically, to be a worrier or warrior

Answers

Answer:

genotypically The genetic makeup, as distinguished from the physical appearance, of an organism or a group of organisms. 2. The combination of alleles located on homologous chromosomes that determines a specific characteristic or trait.

phenotypically   The physical appearance of an organism as distinguished from its genetic makeup. The phenotype of an organism depends on which genes are dominant and on the interaction between genes and environment.Explanation:

Multipotent cells can only give rise to closely related cells.

True or
False

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Answer: false

Explanation:

HELP PLZ PLZ PLZZZZ I need help

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Answer:

Ovulation happens every month for women of childbearing age.  During this phase, the egg is released from one of the ovaries and travels from the fallopian tube to your uterus.

Look it up not only on brainly

7. What is this enzymes optimum temperature?
Enzyme Activity vs. Temperature

a. 60 degrees Celsius
b. 30 degrees Celsius
c. 10 degrees Celsius
d. 40 degrees Celsius

Answers

C. 10 degrees celsius all the other one don’t make sense

The material that a rubber ball is made of is different from the material that makes up something like a pool table ball because it allows the ball to bounce. What type of energy is in this material?
=

Answers

Answer:

h7rhocrbix rcrcrxineox rx ex e crc

rxnrikcjricrc

Explanation:

deibzienizjei xebiebsie is i3 she su 3u d4

What is the era of the multicellular algae

Answers

Answer:

its the phanerozoic era

When the moon orbits the Earth and the_____of the moon shines on the Earth we have a___eclipse.

Answers

Answer:

Shadow, an d total

Explanation:

Its science! Give at least three sentences please and thank ya ill give brainliest Explain how precipitation happens. Use complete sentences and give at least two supporting details.

Answers

Answer:

Precipitation happens as a result of water evaporating and condensing. When water evaporates the water molecules transfer to a gas state into the atmosphere as water vapor. This water vapor then condenses due to cooler air temperatures because of this condensation larger water droplets form. Once these droplets become so large they can no longer be considered water vapor they will fall back down to earth. The act of these droplets falling is what is known as precipitation.

In the autumn, many birds will collect seeds and then hide them in various caches throughout their habitat. The birds will guard their caches
from other birds, and move the caches if they start to dry out. These caches provide a food source for the birds during the water. What is an
ablotic factor that contributed to the evolution of this set of behaviors?
OA cold winter climate
OB competition with other birds for space
OC being preyed upon by larger birds
OD. limited food availability

Answers

Answer:

OA Cold winter climate

Explanation:

Other answers are biotic (living) factors, but cold winter climate is abiotic (non - living).

What is The abiotic (non-living) part of the Fennec foxes environment that this
adaptation (structural adaptation) helps the fox to survive ?

Answers

Answer:

Desert Adaptations Their nocturnal habits help them deal with the searing heat of the desert environment, and some physical adaptations help as well. Their distinctive, bat like ears radiate body heat and help keep the foxes cool.

Explanation:

Animal Adaptations and Survival ... Their large ears, which are usually 6 inches long (15 centimeters), help dissipate excess body heat on hot days in the desert. 2. The fennec fox seems to be the only carnivore living in the Sahara Desert able to ... Fennec foxes also have thick fur on the soles of their feet, which insulate ...

please help , give me a good paragraph & ill give you extra points

Answers

Answer:

kinetic potential energy is turned into speed as it slides down the hill

the more kinetic energy the ball had the further it moved the cup

Explanation:

Ozone in the troposphere is a good thing.
True or false

Answers

Answer:

(False I think)

But in the troposphere, near ground-level, ozone molecules are both air pollutants, threatening the health of living things, and greenhouse gases, trapping heat and contributing to climate change. A small amount of ozone does occur naturally at ground level. Plants and soil release some.

Answer:

false i beileve. i hope this really helps !

Explanation:

Write your own example that includes Newton's three laws of motion. Be sure to make note of each law in your example.

Answers

Answer:

Newton´s third Law Ex: when you push against a wall its pushes back with the exact same force.

Newton´s second Law Ex: If you roll a big ball and a small ball, the small ball  will have more accelration.

Newton´s First Law Ex: If a baseball is hit it wouldn´t stop unless another force stops it or obstruct it.

Explanation:

Hope This Helps :)

One characteristic of an El Niño event is _____.

a. thermohaline circulation ceasing in the Pacific
b. formation of Antarctic Bottom Water ceasing
c. low atmospheric pressure intensifying over the eastern Pacific
d. downwelling occurring in the central Pacific
e. high atmospheric pressure intensifying over the eastern Pacific

Answers

Answer:

During an El Niño event, the surface waters in the central and eastern Pacific Ocean become significantly warmer than usual. ... It also reduces the upwelling of cooler, nutrient-rich waters from the deep—shutting down or reversing ocean currents along the equator and along the west coast of South and Central America.

Explanation:

Question 6 of 10
Imagine you are a detective examining a crime scene. You are trying to
identify the person who committed the crime, so you collect many samples at
the crime scene. Which sample would be most useful in identifying the
person who committed the crime?
A. Pieces of flooring from the crime scene
B. Drops of blood found at the crime scene
C. A watch and keys found at the crime scene
D. Plants growing at the crime scene
SUBMIT

Answers

The sample that would be most useful in identifying a person who committed the crime is drops of blood found at the crime scene. The correct option is B.

What is a crime scene?

A crime scene is a term for the place or location where the crime has been committed. A crime scene contains physical evidence related to the crime, sometimes the body of the victim.

Drops of blood contain the DNA, a sample of blood would be a piece of good evidence to catch the criminal.

Thus, the correct option is B. Drops of blood found at the crime scene.

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Which of the following choices did Wegner give as evidence for continental drift?

a) Fit of continents, rock sequences, glacial evidence
b) Identical fossil species on different continents (both plant and animal), Antarctic climate changes
c) Sea-floor spreading, mid-ocean ridge, and trenches
d) a and b only

Answers

............The answer is D

The Wegner effect is Fit of continents, rock sequences, glacial evidence,  Identical fossil species on different continents (both plant and animal), Antarctic climate changes.

What is Wegner effect?

Wagner's law, commonly referred to as the law of increasing[a] state activity  states that public spending rises as GDP does.  Adolph Wagner (1835–1917), a German economist, is credited with discovering it first in his own nation before noticing it in other nations.

According to Wagner's law, welfare states develop from free-market capitalism because as income rises, more people vote for social services. 

Therefore, The Wegner effect is Fit of continents, rock sequences, glacial evidence,  Identical fossil species on different continents (both plant and animal), Antarctic climate changes.

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How is a mean established?

Answers

Answer:

1 : to institute (something, such as a law) permanently by enactment or agreement. 2 obsolete : settle sense 7. 3a : to make firm or stable. b : to introduce and cause to grow and multiply establish grass on pasturelands.

two multipule choich questions

Answers

Answer:

B. Attach to a host cell

A.

Explanation:

pls help! past due

Which solution is hypotonic?

Which solution is hypertonic?
A) the inside of the cell
B) Neither
C) both
D) the beaker

Answers

Hypotonic - the inside of the cell
Hypertonic - the beaker

Answer:

D I believe I could be wrong

Explanation: Hypertonic solution: A solution that contains more dissolved particles (such as salt and other electrolytes) than is found in normal cells and blood.

Convert to scientific notation: 135,000
a. 1.35 x 10
b. 1.35 x10^2
c. 1.35
d. 1.35 x 10^5

Answers

I think its the letter B
Other Questions
Which event caused the hundred years war Marathon runners:A tend to have more slow twitch muscle fibersB can run only run one or two marathons a yearC are always very thin D only way carbohydrates An anthropologist has been researching how Native Americans in California were impacted by Spanish missionization from 1776 to 1830. The researcher is particularly interested in how Native Californians may have negotiated their identity and balanced traditional practices with colonial influences. Therefore, the anthropologist excavates material remains from areas where the Native Californians may have gone when they fled the Spanish missions. The materials include things such as stone tools, shell beads, and historic bottle glass. These materials are analyzed and used in conjunction with historical documents to help the researcher better understand what life was like for Native Californians at that time.1. What is the primary field of anthropology addressed in this research?2. Are there any other fields of anthropology addressed in this research?3. What aspects of context must be considered as part of this research?4. How might this research contribute to a comparative approach? How did westward expansion affect Native American tribes? aNative American hunters were able to use the railroads to sell bison meat and hides for more money. bAmerican settlers killed many bison, greatly lowering the population of this Native American food source. cAmericans brought enslaved people with them to the west to do farm work. This caused the bison population to increase. dBecause American settlers created many new farms, Native Americans started eating grain as well as bison meat. Determine the stopping location of the prize wheel. At this moment it is centered on the number 5. It is spinning with an energy of 6.19 J. It is slowing at a rate of 1.800 rad/s/s. The disk is made from a wood with a density of 478.0 kg/m^3. The disk is 29.0 mm thick and has a radius of 33.0 cm. Predict the angular displacement it will go through and predict the number on which the wheel will stop. To be safe, you will also get one number the right and to the left of the number you chose. The wheel will spin clockwise. The wheel goes from 1 to 36. HELPPPPPPPPPPPPPPPPPPPPP Jasmine evaluated the expression 3x+2 when x=5.3x+235+237Explain Jasmines mistake. .........yo help plz........ PLEASE HELP!! Write the equation of the line below in slope-intercept form. Remember, write any slopes as reduced, improper fractions! What is the answer whats the sum? How do you find the equation of each function? Function A is f(x)=3x^2 and Function C is f(x)=18x-16. How do you get those? And then how do you get the equation for Function B? Thanks! Read the passage from "Not by Math Alone by Sandra Day OConnor and Roy Romer.A healthy democracy depends on the participation of citizens, and that participation is learned behavior; it doesnt just happen. As the 2003 report "The Civic Mission of Schools" noted: "Individuals do not automatically become free and responsible citizens, but must be educated for citizenship." That means civic learningeducating students for democracyneeds to be on par with other academic subjects.This is not a new idea. Our first public schools saw education for citizenship as a core part of their mission. Eighty years ago, John Dewey said, "Democracy needs to be reborn in every generation and education is its midwife."What message do the writers want to convey in this passage? The responsibility of schools is to focus on teaching academic subjects, not civic learning. Public schools no longer see it as their mission to educate students for civic participation.Democracy is fragile and needs to be reborn in every generation through education.Civic education is essential for a functioning democracy, as citizenship is not automatic but learned. blake went on a bike tour he rode 780 miles in 12 days he rode the same number of miles each day write an expression that can be used to find how many miles blake rode each day Which branch of government isresponsible for making & carrying outthe laws in a parliamentary system ofgovernment?courtsmonarchpresidentlegislature Uma wants to buy a video game system for $270. She has $60 and is saving $30 each week. Solve the equation 30w + 60 = 270 to find how many week w it will take Uma to save enough to buy the system. A. 8 weeks B. 6 weeksC. 7 weeks D. 9 weeks Solve the system of equations by elimination. Show all work neatly on your work page. Checking your answer is wise. :)6x + 3y = -12-6x - 2y = 4 what is the graetest commom factor for 6 and 36 I need help with these questions(see image). Please show workings. Three students, Linda, Tuan, and Javier, are given five laboratory rats each for a nutritional experiment. Each rat's weight is recorded in grams. Linda feeds her rats Formula A, Tuan feeds his rats Formula B, and Javier feeds his rats Formula C. At the end of a specified time period, each rat is weighed again, and the net gain in grams is recorded. Using a significance level of 10%, test the hypothesis that the three formulas produce the same mean weight gain. Linda's rats Tuan's rats Javier's rats43.5 47.0 51.239.4 40.5 40.941.3 38.9 37.946.0 46.3 45.038.2 44.2 48.6Determine whether or not the variance in weight gain is statistically the same among Javiers and Lindas rats. Test at a significance level of 10%. Food that has been prepared or handled should never be placed on thesalesfloor before it has been properly chilled to ensure the internal temperatureis 51F or less.True or False