Answer:
que pregunta?
Explanation:
How/why does the DNA separate? Discuss porosity
or pores, electricity, DNA's charge & size of DNA
pieces. Highlight each term.
Answer:
Gel electrophoresis is a technique used to separate DNA fragments (or other macromolecules, such as RNA and proteins) based on their size and charge. Electrophoresis involves running a current through a gel containing the molecules of interest. Based on their size and charge, the molecules will travel through the gel in different directions or at different speeds, allowing them to be separated from one another.
All DNA molecules have the same amount of charge per mass. Because of this, gel electrophoresis of DNA fragments separates them based on size only. Using electrophoresis, we can see how many different DNA fragments are present in a sample and how large they are relative to one another. We can also determine the absolute size of a piece of DNA by examining it next to a standard "yardstick" made up of DNA fragments of known sizes.
What is a gel?
As the name suggests, gel electrophoresis involves a gel: a slab of Jello-like material. Gels for DNA separation are often made out of a polysaccharide called agarose, which comes as dry, powdered flakes. When the agarose is heated in a buffer (water with some salts in it) and allowed to cool, it will form a solid, slightly squishy gel. At the molecular level, the gel is a matrix of agarose molecules that are held together by hydrogen bonds and form tiny pores.
At one end, the gel has pocket-like indentations called wells, which are where the DNA samples will be placed:
Before the DNA samples are added, the gel must be placed in a gel box. One end of the box is hooked to a positive electrode, while the other end is hooked to a negative electrode. The main body of the box, where the gel is placed, is filled with a salt-containing buffer solution that can conduct current. Although you may not be able to see in the image above (thanks to my amazing artistic skills), the buffer fills the gel box to a level where it just barely covers the gel.
The end of the gel with the wells is positioned towards the negative electrode. The end without wells (towards which the DNA fragments will migrate) is positioned towards the positive electrode.
How do DNA fragments move through the gel?
Once the gel is in the box, each of the DNA samples we want to examine (for instance, each PCR reaction or each restriction-digested plasmid) is carefully transferred into one of the wells. One well is reserved for a DNA ladder, a standard reference that contains DNA fragments of known lengths. Commercial DNA ladders come in different size ranges, so we would want to pick one with good "coverage" of the size range of our expected fragments.
Next, the power to the gel box is turned on, and current begins to flow through the gel. The DNA molecules have a negative charge because of the phosphate groups in their sugar-phosphate backbone, so they start moving through the matrix of the gel towards the positive pole. When the power is turned on and current is passing through the gel, the gel is said to be running. [What voltage is used to run a gel?]
DNA samples are loaded into wells at negative electrode end of gel.
Power is turned on and DNA fragments migrate through gel (towards the positive electrode).
After the gel has run, the fragments are separated by size. The largest fragments are near the top of the gel (negative electrode, where they began), and the smallest fragments are near the bottom (positive electrode).
DNA samples are loaded into wells at negative electrode end of gel.
Power is turned on and DNA fragments migrate through gel (towards the positive electrode).
After the gel has run, the fragments are separated by size. The largest fragments are near the top of the gel (negative electrode, where they began), and the smallest fragments are near the bottom (positive electrode).
Based on similar diagram in Reece et al.^2
2
squared
As the gel runs, shorter pieces of DNA will travel through the pores of the gel matrix faster than longer ones. After the gel has run for awhile, the shortest pieces of DNA will be close to the positive end of the gel, while the longest pieces of DNA will remain near the wells. Very short pieces of DNA may have run right off the end of the gel if we left it on for too long (something I've most definitely been guilty of!).
Visualizing the DNA fragments
Once the fragments have been separated, we can examine the gel and see what sizes of bands are found on it. When a gel is stained with a DNA-binding dye and placed under UV light, the DNA fragments will glow, allowing us to see the DNA present at different locations along the length of the gel.
Explanation:
Hope this helps! :D
why do parents look After their children? give two reason.
Well it is because one;
They want their children to be safe and not hurt.And two;
They don't want there children to get things that are not safe for them.And another is;
It is there responsibility to take care of their children.Which sentence from the section "The Very First Haunted Houses" BEST explains WHY parents wanted to scare kids?
A
American parents tried to come up with ways to distract young pranksters, whose holiday pranks were getting dangerous.
B
Haunted houses "came in about the same time as trick-or-treat did," she says.
C
Kids would travel from basement to basement and experience different scary scenes.
D
A 1937 party guide described how parents could design "trails of terror" to spook their children.
The sentence from the section "The Very First Haunted Houses" that best explains why parents wanted to scare kids is:
A. American parents tried to come up with ways to distract young pranksters, whose holiday pranks were getting dangerous.
According to the section "The Very First Haunted Houses," the reason behind Halloween haunted houses is how dangerous the pranks were getting.Parents decided they needed to do something to distract pranksters.So they would decorate their homes in scary ways and have kids go around seeing different horror scenes.Even though the first haunted houses were very basic, they worked like a miracle and kept children distracted.Learn more about the topic here:
https://brainly.com/question/24499052?referrer=searchResults
What does Canada and climate change have to do together:
4-5 sentences
Answer:
Over the years with increasing temperatures from centuries Canada's weather temps has increased highly. You see, Canada has always been a country with low temperatures. With global warming and carbon dioxide being reused for decades causes build-up in the atmosphere and since that reused carbon has been through the layers (of the atmosphere) it releases higher tempratures. Carbon can reach the thermosphere. But you may be wondering why Canada is still a cold place if the thermosphere is the hottest layer. That's because CO2 is the primary gas that radiationally cools the thermosphere, but at solar maximum, increases in other minor gases cause CO2 cooling to be less important. The "build-up" in the atmosphere gets released because that's how we are still alive.
Explanation:
2. Which word below is a vivid synonym for the following description?
complicated, perplexing, intricate
evident
convoluted
eloquent
relaxing
Is there always someone or something to blame, or are some things truly inevitable?
Answer: I like to think there is always something going on on the universe that we can blame for a bad day or a bad coffee.
Explanation:
But no one really knows if it is inevitable if there is someone or something really controlling our lives.
What is the Denotation of the movie Forrest Gump?
Answer:
His last name means "to clown", as many he encountered felt he was doing, rather than it just being his natural personality.
What is the Conflict on The Reefs By Marcella Morris’s?
Answer:
Sophie thinks the scientists will make fun of her.
Explanation:
The story
Match the characters to the way they develop the plot
9. Which 3 cleaning products do we use in school & for what? 1..... 2......... 3.........
Answer:
disinfectants to clean the toilets, water and soap for cleaning the floor
Which two statements should be included in a summary of the passage?
Answer:
A good summary of an essay should probably include the main idea of each paragraph, and the main evidence supporting that idea, unless it is not relevant to the article or essay as a whole.
Explanation:
Answer: The answers are •The sea repeatedly batters Crusoe, but he does not give up hope for survival.•Because of rough and powerful waves, Crusoe struggles to get to shoreExplanation: it states in the passage that he kept getting pushed back by the waves, and that he keeps going until he made it to shore
Explanation:
How often {you clean ] the windows this year
Answer:
0
Explanation:
im gonna go work at a fast food place not a window cleaner
Answer:
2-3 times a year
Explanation:
Is the narrator in "The Tell-Tale Heart" by Edgar Allan Poe round or flat? How do you know? Include text evidence.(I know they're a round character but can't find evidence)
Answer:
I do not have the answer for you.
Explanation:
Ask these questions to yourself: Do they speak of themselves often? do you know very much about them? if so find a few things they discuss about themselves and use that as an example. If they do not speak of themselves they are a flat character. You must find more than one example that would say they are round, otherwise they are not.
What is the immediate effect of brutes speech on the crowd
Answer:
one of the most effects would be people would get afraid for the most case this will happen. but for other cases there might be attitudinal problem or we can say neglegence
Which meaning defines the word subterranean? (1 point)
O acting as a replacement
O below ground
o frightening
o having both land and water
Tuan likes...a comic after school
Answer:
reading
is the required answer
Summarize what happened to McCandless in Detrital Wash and how he felt about it.
Answer: McCandless arrived in an area of Lake Mead called Detrital Wash on July 6, 1990, and got caught in a flashflood that got his engine wet. He buried the rifle he was carrying and burned all his money. The narrator cites McCandless's diary to substantiate these details
more than fifty miles per hour phrase or clause
Answer:
it is a phrase
Explanation:
Many people say that but they aren't really going more than fifty miles per hour
What type of account is used to pay for daily expenses?
1. Pending account
2. Available account
3. Savings account
4. Checking account
need helpppp
The Current Account is used to pay for daily expenses. It is different other accounts such as the Savings account and the Investment Accounts.
What is a Current Account?A current account is a kind of imprest account which businesses and individuals use to pay for daily and recurrent expenditures.
Savings account on the other hand is used to save for the medium to long term. The correct answer, thus is D: Checking account.
See the link below for more about Checking Accounts:
https://brainly.com/question/24751568
Which of the following events directly result from Romeo’s killing of Tybalt? Select all that apply.
Juliet is to marry Paris on Thursday.
Romeo is banished from Verona.
Juliet stops trusting the Nurse.
Romeo stops believing in fate.
Answer:
She is willing to "visit" death in order to restore her love. Her love is made eternal in the end.
Explanation: Juliet is head over heels for Romeo.
when a person gets hot , his or her skin produces sweat, when a dog gets hot
if u help you get brainlist and a pthank u on ur profile and u get lots of points when u help out
When we lose an organism to extinction, we are no longer able to study it.
What kind of discoveries may we miss out on if an organism goes extinct?
How might the crisis in biodiversity lead to a food crisis for humans?
Answer:
there is a high chance that we may miss out on the evolution of the specimen.
Explanation:
I don't know the second one
Answer:
Hello there,thanks for using brainly! (To whoever keeps camping in my profile stop reporting and deleting my answers its getting annoying)
Explanation:
Answer to number one: When we lose an organism to extinction we loose the chance to be able to study it more thoroughly, see an evolution of the species, identify which animal,insect,etc family it came from, and see what it was capable of doing when it was alive (an example of this was learning that pterodactyls were capable of flying)
Answer to number two:The crisis of biodiversity will lead to a food crisis for humans because:
1.No varieties in certain foods like plants and animals that are healthy
2.The Less food there is,more animals go extinct which means humans will go extinct too.
and finally 3: Without certain types of species like the bee, humans have no chance of survival (since bees pollinate flowers,fruits,and plants which could be used for human consumption and medicine)
Hope this helped
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Dont forget t smash that heart at the bottom <3
Have a great day,Youre amazing!
Beowulf goes to Herot to rid the Danes of Grendel because
A. Beowulf feels it is his duty as a warrior
B. Hrothgar personally sent for his help
C. There is a contest to see who can defeat the monster
D. Beowulf is originally from Denmark
Do the same thing with your second piece of evidence. Write a third paragraph, using stating your evidence, using facts and details from the story, and a quote from the story to prove your explanation.
Answer:
“I don’t see the money . . . and I bet I never shall.” Herbert’s suggestive wish foreshadowed Herbert was going to die, thus creating suspense because the readers would want to know when this event would occur or how. It’s ironic because the reader didn’t expect him to wish for his own death by accident. It also creates suspense for the readers because they want to know what will happen next, how will they deal with the fact their son died in such a horrific way by merely wishing for money.
Is the word as used in the sentence singular or plural?
Civics
A study of civics is helpful in preparing a student for life as an adult.
A.) Plural
B.) Singular
Answer:
a because she is helpful
Explanation:
A study of civics is helpful in preparing a student for life as an adult.
It is singular.
In Unwind by Neal Shusterman, What are the hero’s journey steps?
Please show page numbers.
Answer:
The Hero. The hero of this book is Connor because he is the main character, and is the one to accept the call to adventure.
1. the secretary and treasurer-------- to resign
A: plan B: plans
C: have planned D: were planned
2: most of the money-------- contributed by the children in the elementary school.
A: were spent B: have spent
C: are spent D: was spent
3: Neither the heat of the summer nor the winter winds--------- the dedicated runner.
A: stop B: stops
C: were stopped D: have stopped
4: A letter, accompanied with many mistakes, -------- make a good impression.
A: don't B: haven't C: doesn't D: aren't
Answer:
1. The secretary and treasurer have planned to resign.
2. Most of the money were spent contributed by the children in the elementary school.
3. Neither the heat of the summer nor the winter winds have stopped the dedicated runner.
4. A letter, accompanied with many mistakes, doesn't make a good impression.
Governments often implement price ceilings to protect consumers from the high prices of essential goods and services that frequently follow natural disasters. One
unfortunate side effect of these price ceilings is that they will likely. (1 point
O lead to a shortage as prices are kept from falling to their equilibrium level
• lead to a shortage as prices are kept from rising to their equilibrium level
o lead to a surplus as prices are kept from falling to their equilibrium level
• lead to a surplus as prices are kept from rising to their equilibrium
One unfortunate side effect of these price ceilings is that they will likely lead to a shortage as prices are kept from rising to their equilibrium level.
A price ceiling is a form of price control where the government sets the maximum price of a product. The price of the good is usually set below equilibrium price.
Effects of a price ceiling
It leads to shortages. This is because the profit that can be earned by producers has fallen and this discourages the supply of goods and services. It leads to the development of black markets. Consumer surplus increases. This is because price is below equilibrium price.
To learn more about a price ceiling, please check: brainly.com/question/24312330?referrer=searchResults
Answer:
B) lead to a shortage as prices are kept from rising to their equilibrium level
Help I will give the brainly star
Using the questions below, write a paragraph (5-7 sentences in length) that answers the following questions:
How much slang do you use in general? Which words do you use? How much of the latest slang do you tend to use in your everyday life? Why?
Does your use of slang change depend on where you are or who you are talking to? Explain.
What are your favourite slang words? Why?
Based on the slang words that are popular today, which words do you think will last for years to come? Why?
Are you familiar with Urban Dictionary? Have you ever looked up a word there? Have you ever contributed a word or voted for one?
Answer:
By the way dont mind my bad grammar if theres anything u need to fix feel free to do so
Explanation:
The amount of slang i use on a daily basis can range from medium amounts to larger amounts depending on how im feeling that day and typically I use a variety of slang every day, I like to use slang as a way to shorten the words and also to talk to friends in a funnier manner.
And yes infact the slang i use depends on if im with friends or other people i might switch up what im saying to sound more proper or to just joke around with my homies.
My favorite slang words include, Bussin, Sheesh, Homies, and I know its weird but sussy baka. I think it adds color to what im saying or just makes it a lot funnier. Because Bussin just means good but it sounds funnier than saying this is good because you are saying this is bussin.
I use more of the popular slang so i feel it might be popular for a few years and than die down just like the dab or any other popular moves that people did in like 2018-
In fact i do use the urban dictionary sometimes for the laughs and i do look up slang on there and i can find the definition but no i havent really posted anything onto the urban dictionary or voted for any :)
~~~Hope This Helps!~~~
What kind of abnormality could a 24-year-old woman, who exercised religiously and consumed a healthy diet, possibly have?
Answer:
i believe the answer is b. it makes more sense than the rest
her knowledge of her exercise physiology and the human body
Answer:
b .
Explanation: