What are the answers to these 5 questions?

The correct statements about the experiments that transplanted ferret cortical neural progenitors isolated from different developmental stages into host brains of different ages are; Progenitors show increased fate restriction over time and Progenitors isolated from younger brains can "fate switch" to older neuronal types.

The experiments conducted on ferret cortical neural progenitors showed that the fate of these cells is not intrinsically determined, but rather is influenced by the environment in which they are transplanted. Progenitors isolated from younger brains were able to "fate switch" to older neuronal types when transplanted into older host brains, while progenitors isolated from older brains were not able to "fate switch" to younger neuronal types when transplanted into younger host brains. This suggests that progenitors show increased fate restriction over time, meaning that they become more committed to a specific cell fate as they age.

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Serological methods are used for the detection and enumeration of microorganisms in food by using the specific reactions of antibodies to identify and quantify the presence of specific microorganisms. The most common serological methods used in food microbiology are enzyme-linked immunosorbent assay (ELISA) and lateral flow immunoassay (LFI).

ELISA is a sensitive and specific method that uses an enzyme-linked antibody to detect the presence of a specific microorganism or toxin. The enzyme-linked antibody binds to the microorganism or toxin, and a color change indicates the presence of the target microorganism or toxin.

LFI is a rapid and easy-to-use method that uses a lateral flow strip to detect the presence of a specific microorganism or toxin. The strip contains a specific antibody that binds to the target microorganism or toxin, and a color change indicates the presence of the target microorganism or toxin.

Both ELISA and LFI are widely used in the food industry for the detection and enumeration of microorganisms in food, and they are important tools for ensuring the safety and quality of food products.

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The level of saturation and cis/trans isomerization can influence how closely together fatty acids are packed in foods or biological membranes in a few ways.

First, the level of saturation can affect the packing of fatty acids. Saturated fatty acids have single bonds between all of the carbon atoms in their hydrocarbon chains, which allows them to pack closely together.

This is why saturated fats, such as butter and lard, are solid at room temperature. In contrast, unsaturated fatty acids have one or more double bonds in their hydrocarbon chains, which creates kinks in the chains and prevents them from packing closely together.

This is why unsaturated fats, such as olive oil and canola oil, are liquid at room temperature.

Second, the cis/trans isomerization can also affect the packing of fatty acids. Cis fatty acids have the hydrogen atoms on the same side of the double bond, which creates a bend in the chain and prevents them from packing closely together.

Trans fatty acids have the hydrogen atoms on opposite sides of the double bond, which allows them to pack more closely together. This is why trans fats, such as partially hydrogenated oils, are solid at room temperature.

Overall, the level of saturation and cis/trans isomerization can influence how closely together fatty acids are packed in foods or biological membranes, which can affect their physical properties and functions.

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The translation of UAA CAA GGA GCA UCC will not produce any protein

The translation of UGA CCC GAU UUC AGC will also not produce any protein.

To translate the mRNA transcripts into protein, we need to use the genetic code to convert the mRNA codons into amino acids. Each mRNA codon corresponds to a specific amino acid, and the sequence of codons determines the sequence of amino acids in the protein.

The first codon, UAA, is a stop codon and does not code for an amino acid. Therefore, this mRNA transcript does not produce a protein.

UGA is also a stop codon and does not code for an amino acid.

Therefore, the two mRNA transcripts do not produce proteins.

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CAH can cause abnormalities in the internal ducts, external genitalia, and brains. This can manifest as an abnormally in females, hypospadias in males, and underdeveloped or absent reproductive organs.

Overall, CAH can have a significant impact on the physical and mental health of XX individuals, and it is important for these individuals to receive appropriate medical treatment and support.

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The interference must have been 80%. Option B

The interference must have been 80%. Interference is the phenomenon in which one crossover event prevents or reduces the likelihood of another crossover event occurring nearby. It is calculated using the formula: Interference = 1 - (Observed double recombinants/Expected double recombinants).

In this case, the observed double recombinants are 16 and the expected double recombinants are 80 out of 800 progeny.

Plugging these values into the formula gives: Interference = 1 - (16/80) = 1 - 0.2 = 0.8.

Therefore, the interference must have been 80%, or option b.

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The question is asking about the effects of toxicity on specific organs of the body. Toxicity is unique to each organ, and the effects can be enhanced by distribution features that deliver a high concentration of toxicant to the target organ.

Toxicity is defined as the adverse effects of a substance on specific organs of the body. Each organ has a unique response to toxicity, and the effects may be enhanced by the distribution of the toxicant to the organ.

Additionally, a single toxicant may have several target organs, and the highest concentration of the toxicant is always found in the target organ. This is important to consider when assessing the potential health risks of exposure to toxic substances.

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(1) H&E stands for Hematoxylin and Eosin. Hematoxylin stains acidic structures blue, Eosin stains cytoplasm and extracellular matrix pink.

(2) Pathologists take into account various characteristics of tumors, such as the degree of differentiation of the tumor cells, the growth pattern of the tumor, the presence of necrosis, and the extent of invasion into surrounding tissues, to determine tumor grade.

(3) The purpose of immunohistochemistry (IHC) is to identify specific proteins or other antigens in tissue samples using antibodies that bind to those targets.

(4) The steps involved in immunohistochemical staining for a specific antibody include fixing and embedding the tissue, cutting sections, antigen retrieval, blocking endogenous peroxidase activity, blocking non-specific binding, incubation with the primary antibody, incubation with a secondary antibody conjugated to a detection enzyme, and visualization using a chromogenic or fluorescent substrate. The rationale behind each step is to ensure proper antigen retrieval, minimize non-specific binding, and amplify the signal for visualization.

(5) A specific protein is visually detected by IHC using antibodies that recognize and bind to the target protein in the tissue section. To quantify the amount of protein present, various methods such as visual scoring, image analysis, or digital pathology software can be used.

(6) TMA stands for tissue microarray. TMAs are useful for studying the expression of specific proteins across large numbers of samples and for identifying biomarkers associated with disease.

(7) The advantages of digitally scanning slides compared to classical microscopy include the ability to view and analyze high-resolution images remotely, the ability to share and collaborate on images, etc.

(8) A digital slide is a high-resolution image of a tissue section that has been scanned and stored electronically.

(9) Manual scoring of tissue sections can be time-consuming and subject to inter-observer variability. To address these issues, various software programs have been developed that use image analysis algorithms to quantify staining intensity and distribution, providing more objective and reproducible results.

(10) The biomarkers ER and HER2 are important prognostic and diagnostic markers in breast cancer. ER is a hormone receptor that is expressed in approximately 70% of breast cancers and is associated with a more favorable prognosis.

H&E (Hematoxylin and Eosin) staining is a common method used in histology to visualize the cellular structure and tissue architecture of cancer cells. The staining helps to identify cancerous cells by highlighting their morphological and structural characteristics, such as nuclear abnormalities, irregular cell shape, and high mitotic activity.

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Answer:

The correct option is C: No, because both of her parents could be heterozygous.

Explanation:

No, she does not have any concerns about her parentage, because both of her parents could be heterozygous for the A and B alleles. When an A allele and a B allele are present, they can combine to produce the AB blood type. However, when an A allele and a B allele are absent, the individual will have the O blood type. Therefore, the child could inherit an O allele from each parent, resulting in the O blood type. The correct option is C: No, because both of her parents could be heterozygous.

No she haven't any concerns about her parentage, because both of her parents could be heterozygous.


Blood type is determined by the presence or absence of specific antigens on the surface of red blood cells. There are three main types of antigens, A, B, and O, which determine an individual's blood type.

An individual with type A blood has the A antigen, type B has the B antigen, type AB has both A and B antigens, and type O has neither A nor B antigens.

Each individual inherits one allele for the ABO blood group from each parent. The alleles for the ABO blood group are A, B, and O. The A and B alleles are codominant, meaning that they are both expressed if an individual inherits one of each, resulting in type AB blood.

The O allele is recessive, meaning that it is only expressed if an individual inherits two O alleles, resulting in type O blood.

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The correct gene order for the phoenixes is h, c, y.

To determine the gene order, we need to look at the relative number of observations for each genotype combination. The largest number of observations (2000) is for the genotypes h,y,c and H,Y,C. This means that these genotypes are the most common and likely to represent the parental genotypes.

The next largest number of observations (300) is for the genotypes h,Y,C and H,y,c. These genotypes represent recombinant offspring that have one gene swapped between the parental genotypes. This indicates that the gene that is swapped (y) is the gene that is furthest away from the other two genes (h and c) on the chromosome.

The smallest number of observations (100 and 200) are for the genotypes h,y,C and H,Y,c and h,Y,c and H,y,C. These genotypes represent double recombinant offspring that have two genes swapped between the parental genotypes. This indicates that the two genes that are swapped (h and c) are closer together on the chromosome than the other gene (y).

Therefore, the correct gene order is h, c, y.

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When freezing mammalian cells to create a frozen cell stock, it is important to use a cryoprotectant such as DMSO (dimethyl sulfoxide) or glycerol to prevent ice crystal formation and reduce cell death during the freeze/thaw process.

Additionally, it is important to freeze the cells slowly to allow for the gradual uptake of the cryoprotectant and prevent osmotic stress.

A cell freezing strategy that will achieve 1x10^6 cells/ml can be designed as follows:

This strategy will result in a final cell concentration of 1x10^6 cells/ml after accounting for the expected cell death during the freeze/thaw process.

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Fewer new plants and trees are emerging, diminution of the rainforest ecosystem's diversity, reduction in the variety of plants that orangutans consume and a rise in the number of insects.

Due to a lack of predator control, insect prey species are overpopulated, pest insect infestations that could harm crops or other plants, soil deterioration [EFFECTS]. Nutrient loss on the forest floor as a result of less fertilisation from orangutan poop, decreased soil structure, which increases the risk of landslides. Changes to the canopy's structure [EFFECTS]. Reduced habitat for other animals and insects that live in the canopy as a result of changes in temperature, moisture, and light regimes. Revenue from tourism declining -> [EFFECTS]. Reduction in the amount of money derived from ecotourism by nearby communities, a reduction in funding for initiatives to preserve the rainforest ecosystem. Threat to the survival of orangutans [EFFECTS].

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To determine if an element or a compound passed through the cell membrane, you need to observe the color change of the substance after it has passed through the membrane.

If the iodine was dark blue before passing through the membrane and is now a different color, this means that it has passed through the membrane.

Similarly, if the silver nitrate was white before passing through the membrane and is now a different color, this means that it has passed through the membrane. It is important to note that the color change may not be drastic, but any change in color indicates that the element or compound has passed through the cell membrane.

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Paralogous genes have evolutionary significance as they can provide the source material for new proteins and pathways, allowing organisms to adapt to their environment.

Paralogous genes are genes that have been duplicated within a genome through a gene duplication event. There are some general evolutionary significances of paralogous genes that are worth considering.

One of the primary evolutionary significances of paralogous genes is that they allow for the evolution of new gene functions. When a gene is duplicated, there are now two copies of that gene in the genome.

Another significance of paralogous genes is that they can provide a buffer against deleterious mutations. When there are two copies of a gene in the genome, one copy can take on a new function or role, while the other copy can continue to perform the original function.

Finally, paralogous genes can provide a source of genetic diversity that can help organisms adapt to new environments or challenges. When there are multiple copies of a gene in the genome, there are more opportunities for mutations to occur and for new genetic material to be created.

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Answer:

by combining the DNA of two different organisms

Explanation:

The four F1 progeny all have the same genotype with respect to quip and tub. These can all be described as dihybrids in cis. The given statement refers to a pure breeding male of tub/tub is crossed to a pure breeding female of quip/quip. This will yield four dihybrid F1.

They all have the same genotype when it comes to tub and quip. Hence, these can be described as dihybrids in cis.The two different alleles of the same gene are found on the same chromosome in cis configuration. In this condition, they are inherited together by the offspring from the parent. Therefore, it is considered as the configuration of the dominant allele in cis configuration with the recessive allele.

Antarctica is a continent located in the southern hemisphere. It is the fifth-largest continent on the planet, covering an area of 14 million km². It is uninhabited, except for research purposes by scientists and researchers. Antarctica is known to have some of the coldest climates on earth, with temperatures reaching as low as -128.6°F (-89.2°C).Genes are considered the basic unit of heredity. They are responsible for determining the physical traits of an organism. They are the genetic material present in the DNA sequence that carries information from one generation to another.

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The most likely identification of the rapidly growing hyaline mold is d. Aspergillus fumigatus. This mold is known for its distinctive dark green color on the front and white color on the reverse of the colony. It also has septate hyphae and flask-shaped phialides with clumps of conidia at the tips, which match the description given in the question. Therefore, option d is the correct answer.

A form of filamentous fungus known as hyaline mold, often referred to as aseptate mould or non-septate mold, is devoid of the characteristic septa or crosswalls that are present in the majority of other molds. As a result, the hyphae of these fungi create lengthy, continuous, and branching structures rather than discrete compartments.

In addition to soil, water, and decomposing organic waste, hyaline moulds can also be found in other habitats. Some hyaline mould species are known to infect people, especially those with compromised immune systems. The majority of hyaline molds, however, are not hazardous and are crucial to the breakdown of organic waste and the cycling of nutrients in ecosystems.

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The generation time (g) of a bacterium is the time required for it to divide and form two daughter cells. This is useful information when testing a bacterium's response to an inhibitory substance because it allows us to measure the effect of the substance on the bacterium's growth and reproduction.

If the inhibitory substance is effective, it will slow down or even stop the bacterium's growth and reproduction, leading to an increase in the generation time.

By comparing the generation time of the bacterium in the presence of the inhibitory substance to the generation time in the absence of the substance, we can determine the effectiveness of the inhibitory substance.

Therefore, the generation time (g) of a bacterium is useful information when testing its response to an inhibitory substance because it allows us to measure the effect of the substance on the bacterium's growth and reproduction.

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The process taking place is osmosis, which is a passive transport mechanism that allows the solvent (in this case, water) to move across a semipermeable membrane from an area of high concentration to an area of low concentration.

Since the 2% potassium solution has a lower concentration of solutes compared to the 5% potassium solution inside the cell, water will move from the 2% potassium solution into the cell to equalize the concentration. Therefore, the solvent will move from the 2% potassium solution into the cell.

As a result of water moving into the cell, the cell will become hypotonic (i.e., having a lower concentration of solutes) compared to the 2% potassium solution. This is because the concentration of potassium inside the cell remains unchanged, while the volume of the cell increases due to the influx of water.

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True. Low elevation cheatgrass shows a "drought recovery" strategy by being an annual plant. This is because annual plants, like cheatgrass, are able to complete their entire life cycle in one growing season, allowing them to reproduce and spread their seeds before drought conditions become too severe.

This allows the cheatgrass to recover from drought conditions more quickly than perennial plants, which have a longer life cycle and may not be able to reproduce before drought conditions become too severe.

False. Mid elecation incense cedar does not display a drought avoidance strategy by shifting its P50 lower in dry sites to avoid embolism. Instead, incense cedar displays a drought avoidance strategy by reducing its leaf area and closing its stomata during dry conditions.

This reduces the amount of water lost through transpiration and helps the plant conserve water during drought conditions. The P50 value, which is a measure of a plant's resistance to embolism, does not play a role in the incense cedar's drought avoidance strategy.

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The soft tissues, tendons, ligaments, and bones that make up your musculoskeletal system. They assist you in moving and sustain the weight of your body collectively.

As the musculoskeletal system, the locomotor system is also recognized. The skeleton, skeletal muscles, ligaments, tendons, joints, cartilage, and other connective tissue compose it. Your body can move because of the cooperation of these parts.

Pictures for The "musculoskeletal system" may be used occasionally.

Skeletal muscle, smooth muscle, and cardiac muscle are the three forms of muscle tissue found in the body. Voluntary and striated skeletal muscle. These muscles regulate conscious movement by being attached to bones.

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The time taken for 1000 atoms of iodine-131 to decay to 125 atoms is 16 days

First, we shall determine the number of half lives that has elapsed. This is obtained as follow:

2ⁿ = N₀ / N

2ⁿ = 1000 / 125

2ⁿ = 8

2ⁿ = 2³

n = 3

Finally, we shall determine the time taken for the 1000 atoms of iodine-131 to decay to 125 atoms. Details below

n = t / t½

Cross multiply

t = n × t½

t = 2 × 8

t = 16 days

Thus, the time taken is 16 days

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GMO foods are just as wholesome and secure to consume as their non-GMO equivalents. In fact, several Transgenic plants have undergone modifications to increase their nutritional worth.

Genetically engineered organisms (GMOs) Gene engineering is a crucial component of contemporary biotechnology that is used, among other things, to give bacteria, plants, and animals new traits. This can be accomplished by introducing a gene from, say, a bacteria, into a plant or an animal (transgenes).

To tackle crippling and uncommon diseases, lessen our environmental impact, feed the needy, use less and cleaner energy, and have safer, cleaner, and more effective industrial manufacturing processes, modern biotechnology offers ground-breaking goods and technologies.

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Deamination of 5-methyl-cytosine (5mC) leads to hot spots for spontaneous mutations more than the deamination of cytosine  because 5mC is much more likely to be deaminated due to its higher reactivity, making it more susceptible to spontaneous mutation.

The deamination of 5-methyl-cytosine in DNA leads to hot spots for spontaneous mutations more than the deamination of cytosine because of the differences in the repair mechanisms for these two types of deamination. Deamination is the process of removing an amino group from an amino acid or other compound, and in the case of DNA, it involves the removal of an amino group from a nucleotide base.

When cytosine undergoes deamination, it is converted to uracil, which is recognized as an abnormal base in DNA and is quickly repaired by the DNA repair machinery. However, when 5-methyl-cytosine undergoes deamination, it is converted to thymine, which is a normal base in DNA and is not recognized as a mutation by the DNA repair machinery. As a result, the thymine remains in the DNA and can lead to a mutation if it is not corrected before DNA replication.

Therefore, the deamination of 5-methyl-cytosine is more likely to lead to hot spots for spontaneous mutations because it is less likely to be repaired than the deamination of cytosine. This is why the deamination of 5-methyl-cytosine in DNA leads to hot spots for spontaneous mutations more than the deamination of cytosine.

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Answer:

Variation can be defined as any difference between the individuals in a species or groups of organisms of any species. mutation is the ultimate source of genetic variation, but mechanisms such as sexual reproduction and gene flow contribute to it as well

Explanation:

In a cohort study, a group of individuals with a history of binge drinking would be identified and followed over time.

Another group of individuals without a history of binge drinking would also be identified and followed for comparison. The researchers would then collect data on the incidence of heart disease in both groups and compare the rates between the two groups.

The researchers would also collect data on potential confounding factors, such as age, sex, smoking, and physical activity, to control for their effects.

In a case-control study, individuals with heart disease (cases) and individuals without heart disease (controls) would be identified.The researchers would then collect data on the individuals' history of binge drinking and compare the rates between the cases and controls.

The researchers would also collect data on potential confounding factors and control for their effects using statistical methods. This study design is efficient for studying rare outcomes such as heart disease, but it cannot establish causality.

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Rainfall is the predominant form of precipitation and hence the term precipitation is used synonymously with rainfall. The magnitude of rainfall shows high temporal and spatial variation. This variation is responsible for the occurrence of hydrologic extremes such as floods and droughts.

Rainfall is the most common form of precipitation and it occurs when water vapor in the atmosphere condenses and falls to the ground as liquid water. The amount of rainfall varies greatly depending on the location and time of year. Some areas may receive very little rainfall, while others may experience heavy rainfall events.
The variation in rainfall is responsible for the occurrence of hydrologic extremes such as floods and droughts. Floods occur when an area receives more rainfall than it can handle, causing water levels to rise and overflow onto land. Droughts occur when an area experiences a prolonged period of below-average rainfall, leading to a shortage of water for plants, animals, and humans.
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Paramyxoviruses are a type of RNA virus that can cause a variety of diseases in humans, including measles, mumps, and respiratory syncytial virus (RSV). These viruses have developed several strategies to evade the innate immune system and establish infection.

Four to five immune modulation/evasion strategies found in the articles and the viral proteins that carry out the effects are:

1. Inhibition of interferon signaling: Paramyxoviruses produce proteins such as V, C, and NS1 that can interfere with the signaling pathways of interferons, which are important for antiviral responses.

2. Suppression of apoptosis: Some paramyxoviruses, such as the measles virus, produce proteins that can inhibit the induction of apoptosis in infected cells. This allows the virus to continue replicating and avoid being eliminated by the immune system.

3. Inhibition of antigen presentation: Paramyxoviruses can also produce proteins that interfere with the presentation of viral antigens to immune cells, preventing the activation of the adaptive immune response.

4. Modulation of cytokine responses: Paramyxoviruses can produce proteins that can alter the production and activity of cytokines, which are important for coordinating immune responses.

5. Evasion of natural killer (NK) cells: Some paramyxoviruses, such as RSV, can produce proteins that can prevent the activation of NK cells, which are important for eliminating virus-infected cells.

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The mRNA that could be translated to synthesize a short peptide of five amino acids is  5'-AUGCGUCGUAGCUUAUCGUCUCGUGAUGCUGAUC-3'. (3)

This mRNA sequence contains the start codon AUG, which signals the start of translation, and is followed by four more codons that code for amino acids.

The sequence also contains a stop codon UGA, which signals the end of translation. Therefore, this mRNA sequence could be translated to synthesize a short peptide of five amino acids.

To summarize, the correct answer is:
Option 3: 5'-AUGCGUCGUAGCUUAUCGUCUCGUGAUGCUGAUC-3'

This mRNA sequence contains the start codon AUG, followed by four more codons that code for amino acids, and ends with a stop codon UGA. Therefore, it could be translated to synthesize a short peptide of five amino acids.

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1- The given statement "Cancer Stem Cells adhere to the stem cell model by self-renewing and simultaneously generating progenitors that have lost their stemness." is true because cancer Stem Cells do adhere to the stem cell model by self-renewing and simultaneously generating progenitors that have lost their stemness. This is one of the defining characteristics of Cancer Stem Cells.

2- The given statement "The frequency of Cancer stem cells can be determined easily" is false because the frequency of Cancer stem cells cannot be easily determined.  This is because Cancer Stem Cells are rare and difficult to isolate. Additionally, there is no universal marker for Cancer Stem Cells, so it is difficult to accurately measure their frequency.

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