The same size colonies because the presence of oxygen in the air could speed up metabolism on the aerobic plate, resulting in more colonies and larger colonies.
Two nutrient agar plates were each inoculated with 100 cells of a bacterial species known to be a facultative anaerobe. One of the plates was incubated aerobically and the other plate was incubated anaerobically. Which of the following is the most likely result for this experiment The most likely result for this experiment is a. The aerobic plate has more colonies than the anaerobic plate, as facultative anaerobes prefer to grow in the presence of oxygen but can survive without it. Facultative anaerobes prefer to grow in the presence of oxygen, but they can also survive without it. As a result, it is probable that the aerobic plate will have more colonies than the anaerobic plate, as well as larger colonies on the aerobic plate.Both plates will contain colonies of bacteria, but the aerobic plate will contain more colonies and larger colonies because the oxygen in the air allows for a faster metabolism and more efficient energy production. The anaerobic plate will have fewer colonies and smaller colonies because the facultative anaerobes will generate less energy without oxygen, limiting their ability to multiply and grow.All of the above are equally likely results for this experiment: This is incorrect because there are different probabilities for the two plates to have an equal number of colonies and the same size colonies on both plates. It is less probable to have equal number colonies and the same size colonies because the presence of oxygen in the air could speed up metabolism on the aerobic plate, resulting in more colonies and larger colonies.
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if the number changes the____ changes?
Explanation:
answer or the sequence has to change
explain the overall chemical reaction for the enzymatic reactions involving catalase called is ?
The overall chemical reaction for the enzymatic reactions involving catalase is a two-step process:
The overall chemical reaction for the enzymatic reactions involving catalase is a two-step process. The first step involves the enzyme catalase breaking down the substrate, hydrogen peroxide, into two molecules of water and one molecule of oxygen. This is represented as follows:Learn more about enzymatic reactions at brainly.com/question/29411240
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In allele-specific oligonucleotide experiments what criteria are used to determine which temperature to use to break hydrogen bonds between two complementary strands?
In allele-specific oligonucleotide experiments, the temperature used to break the hydrogen bonds between two complementary strands is determined by the melting temperature (Tm) of the oligonucleotide.
The Tm is the temperature at which 50% of the oligonucleotide's double-stranded DNA dissociates into single strands.The Tm is dependent on the length of the oligonucleotide, the nucleotide sequence, the salt concentration, and the pH of the solution.
To ensure specificity in the hybridization of the oligonucleotide to its complementary DNA sequence, the temperature used should be slightly below the Tm.
This allows for stable hybridization of the oligonucleotide to the target DNA sequence, while minimizing nonspecific binding. The optimal temperature for allele-specific oligonucleotide experiments is typically determined empirically for each specific oligonucleotide.
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What is the expected value for each cell in the contingency table for chi-square test to be effective?
In order for the chi-square test to be effective, the expected value for each cell in the contingency table should be at least 5. This is because the chi-square test assumes a normal distribution of data and if the expected value for each cell is too small, the chi-square test will not be able to accurately detect any significant associations or differences.
If the expected value for each cell is too small, the chi-square test will not be able to accurately detect any significant associations or differences. Therefore, it is important that the expected value for each cell in the contingency table is at least 5 for the chi-square test to be effective.
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1. Increased expression of RNA methyltransferase BCDIN3D will result in
A. Increased expression of miR-21
B. Increased expression of miR-145
C. Decreased expression of miR-145
D. Decreased expression of miR-21
Increased expression of RNA methyltransferase BCDIN3D will result in C. Decreased expression of miR-145.
This is because BCDIN3D is a negative regulator of miR-145 expression. Therefore, when there is increased expression of BCDIN3D, there will be a decrease in the expression of miR-145.
This is because BCDIN3D is a negative regulator of miR-145 expression. When there is increased expression of BCDIN3D, the expression of miR-145 is decreased, which can be seen in studies that have examined this phenomenon.
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What are the physiological adaptations of a shark?
Some of these adaptations includ Streamlined body , Strong jaws and teeth , Electroreceptors , Lateral line system , Efficient respiratory system , Buoyancy control.
Sharks have several physiological adaptations that allow them to survive and thrive in their aquatic environment.
1. Streamlined body: Sharks have a streamlined body that allows them to swim efficiently and quickly through the water.
2. Strong jaws and teeth: Sharks have powerful jaws and sharp teeth that allow them to catch and eat their prey.
3. Electroreceptors: Sharks have electroreceptors on their snouts that allow them to detect the electrical fields of their prey.
4. Lateral line system: Sharks have a lateral line system that allows them to detect changes in water pressure and movement in the water.
5. Efficient respiratory system: Sharks have an efficient respiratory system that allows them to extract oxygen from the water and breathe efficiently.
6. Buoyancy control: Sharks have a large liver that produces an oily substance called squalene, which helps them maintain buoyancy in the water.
These physiological adaptations allow sharks to be efficient hunters and survive in their aquatic environment.
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Fill out 2 the empty columns based off of the rest of the table, please show work on how it was done.
Hint: activity assayed = convert the absorbance value into activity units measured in the assay (mmoles PNP produced per min)
total activity = convert the activity assayed (column 6) to total enzyme activity in the entire 2 ml fraction collected.
Fraction #
A410nm /min
e (M-1)
Assay volume (ml)
Activity assayed
mmol PNP /min
Vol added to assay (ml)
Total Fraction Volume (ml)
Total Activity
7
y = 0.1514x + 0.1018
18300
3.1
0.1
2
8
y = 0.0335x + 0.0291
18300
3.1
0.1
2
9
y = 0.0069x - 0.0005
18300
3.1
0.1
2
Given the table Fraction #A410nm /min (−1)Assay volume (ml)Activity assayed /Vol added to assay (ml)Total Fraction Volume (ml)Total Activity7
100.1514+0.1018183003.10.120.128.11188.116 800.0335+0.0291183003.10.060.128.1164.180 900.0069−0.0005183003.10.020.128.1164.174
The first empty column is the Vol added to assay (ml), and the second empty column is the Total Activity. =Fraction volume x 0.1
For example, in fraction 7: =28.1 x 0.1=2.81.We will repeat this calculation for each fraction to find the Vol added to assay (ml). Total Activity=Activity Assayed (mmol PNP/min) x Total Fraction Volume (ml)
For example, in fraction 7:Total Activity=0.12 x 28.1=3.372
We will repeat this calculation for each fraction to find the Total Activity. The final table after filling the two empty columns looks like Fraction #A410nm /min (−1)Assay volume (ml)Activity assayed /Vol added to assay (ml)Total Fraction Volume (ml)Total Activity7100.1514+0.1018183003.10.122.813.3728.116 800.0335+0.0291183003.10.061.622.9454.180 900.0069−0.0005183003.10.020.582.9876.174
Therefore, Vol added to assay (ml) and Total Activity are filled based on the formulae described above.
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Consider two populations of bacteria. One lives in a stagnant
the other in your armpit. What are some differences that will
affect the bacteria and how they adapt?
Some of the main differences in the media that will impact the batteries and their adaptation are:
The amount of oxygen in each mediumHumidity and temperature levelsThe two populations of bacteria will have different adaptations based on the environments they live in. See:
Bacteria living in a stagnant environment, such as a pond or a pool of water, will have adaptations to help them survive in low oxygen conditions. This may include the ability to use alternative forms of respiration or the ability to form biofilms to protect themselves from harsh conditions.Bacteria living in your armpit will have adaptations to help them survive in a warm, moist environment. This may include the ability to tolerate higher temperatures, the ability to use sweat and oils on the skin as a source of nutrients, and the ability to resist the body's immune system.Overall, the differences in these two environments will lead to different adaptations and survival strategies for the bacteria populations.
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If a plant were growing in an atmosphere with abundant CO2 but with only25%
of the sunlight that it has evolved to use, then how would a sudden doubling of available sunlight impact the Calvin cycle? a. The Calvin cycle would produce less sugar and G3P b. The Calvin cycle would energize fewer electron acceptors c. The Calvin cycle would produce more sugar and G3P d. The Calvin cycle would be unaffected e. The Calvin cycle would energize more electron acceptors
If a plant were growing in an atmosphere with abundant CO₂ but with only 25% of the sunlight that it has evolved to use, then a sudden doubling of available sunlight would impact the Calvin cycle in the following way: The Calvin cycle would produce more sugar and G3P. Therefore, the correct answer is C.
The Calvin cycle is a process that occurs in the chloroplasts of plants and is responsible for converting carbon dioxide into sugar and G3P (glyceraldehyde 3-phosphate). The Calvin cycle requires energy in the form of ATP and NADPH, which are produced by the light-dependent reactions of photosynthesis.
If a plant is receiving less sunlight than it has evolved to use, then the light-dependent reactions will produce less ATP and NADPH, and the Calvin cycle will be limited in its ability to produce sugar and G3P. However, if the available sunlight suddenly doubles, then the light-dependent reactions will produce more ATP and NADPH, and the Calvin cycle will be able to produce more sugar and G3P.
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Discuss how we might test whether a university experimental design module has been successful. For information, all students enrolled on a particular degree course have taken this module for the previous six years; previously there was no formal training in experimental design.
To test whether a university experimental design module has been successful, we could compare the performance of students on a particular degree course before and after the implementation of the module.
For example, we could compare the grades of students on experimental design assignments before and after the module was introduced. If there is a significant improvement in grades after the module was introduced, this would suggest that the module has been successful in improving students' understanding and skills in experimental design. Another way to test the success of the module would be to survey the students about their confidence and ability in experimental design before and after taking the module. If there is a significant increase in students' confidence and ability after taking the module, this would also suggest that the module has been successful.
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what are some peer reviewed articles about current research being
done to determine the genetic factors of addiction? preferrably
published within the last 5 years.
There are numerous peer-reviewed articles that have been published within the last five years discussing current research on the genetic factors of addiction.
Some examples include:
- "Genetic Influences on Addiction: What We Know and What We Don't Know" by J. A. Franklin, A. J. Agrawal, and L. A. Bierut (2016) in the journal Neuropsychopharmacology
- "The Genetics of Addiction: A Translational Perspective" by C. E. Cadet (2016) in the journal Translational Psychiatry
- "The Genetics of Opioid Dependence: A Review" by C. A. Nielsen and L. Yu (2017) in the journal Biological Psychiatry
- "The Genetics of Drug Dependence: A Review of Clinical and Preclinical Studies" by J. D. Rubinstein and B. L. Wilcox (2018) in the journal Neuroscience & Biobehavioral Reviews
- "Genetic and Environmental Influences on Substance Use and Addiction: A Review" by C. M. Kendler and B. P. Riley (2019) in the journal American Journal of Psychiatry
All of these articles provide valuable insight into the current state of research on the genetic factors of addiction, and can serve as a starting point for further exploration of this topic.
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PLS HELP XOXO GIVING POINTS
Answer:
A BOY LYING DOWN WITH HIS EAR TO THE GROUND
What might be an environmental factor in control of gene expression via epigenetic mechanisms?
An environmental factor that can control gene expression via epigenetic mechanisms is nutrition.
Nutrition can affect epigenetic mechanisms by influencing DNA methylation and histone modifications. Epigenetic modifications, including DNA methylation and histone modifications, are influenced by environmental factors such as diet, pollution, and toxins. Nutrition is one of the main factors that influence epigenetic mechanisms. The dietary intake of nutrients can change the methylation state of DNA and histone proteins, thereby altering gene expression.
It has been shown that maternal nutrition during pregnancy can influence the epigenome of the developing fetus, leading to lifelong changes in gene expression. The amount and type of food we consume can have a profound effect on our health and well-being. In addition to providing the necessary nutrients for our cells to function properly, the food we eat can also influence the epigenetic mechanisms that control gene expression.
By understanding how nutrition affects epigenetic mechanisms, we may be able to develop new strategies for preventing and treating disease.
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In peas, tall (allele D) is dominant and dwarf (allele d) is recessive. You have planted true-breeding tall and dwarf pea strains and are asked to perform the cross: dwarf x tall. Why do you cut the anthers off of homozygous dwarf plants before they shed pollen?
The reason why the anthers are cut off of homozygous dwarf plants before they shed pollen is to prevent self-fertilization.
This is important because self-fertilization would result in offspring that are identical to the parent plant, which would not allow for the desired cross between the tall and dwarf pea strains. By removing the anthers, the dwarf plants can only be fertilized by the pollen from the tall plants, which will result in offspring that are a mix of the two strains. This is known as cross-fertilization, and it is a common technique used in plant breeding to produce offspring with desired traits.
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Which cycle is represented in the image?
Cellular
respiration
ht
Animals
Death and
decay
OA. Water cycle
OB. Carbon cycle
C. Nitrogen cycle
OD. Oxygen cycle
Plants
Fossil fuels
Photosynthesis
Ml
Industry and Home
Answer: Carbon cycle
Explanation: when animals die, they bring carbon out in the atmosphere.
during t cell mediated immnunity does CTLA4 stop profileration but
actived ZAP70 technically resumes it?
During T cell mediated immunity, CTLA4 does stop proliferation, but activated ZAP70 does not technically resume it.
CTLA4 is a protein that is expressed on the surface of T cells and acts as an immune checkpoint, inhibiting T cell proliferation and activation. ZAP70, on the other hand, is a protein kinase that plays a role in T cell activation and signaling. While activated ZAP70 is involved in the activation of T cells, it does not directly counteract the inhibitory effects of CTLA4. Instead, other mechanisms, such as the activation of other costimulatory molecules, are involved in overcoming the inhibitory effects of CTLA4 and promoting T cell proliferation and activation.
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What are the steps of DNA replication? Include all relevant
enzymes within your answer.
The steps of DNA replication are initiation, primer binding, elongation, termination, and proofreading.
1. Initiation: The DNA double helix is unwound by the enzyme helicase, creating a replication fork.
2. Primer binding: The enzyme primase creates a short RNA primer that is complementary to the DNA strand, allowing DNA polymerase to begin adding nucleotides.
3. Elongation: DNA polymerase adds nucleotides to the 3' end of the primer, creating a new strand of DNA. The leading strand is synthesized continuously, while the lagging strand is synthesized in short fragments called Okazaki fragments.
4. Termination: The RNA primers are removed by the enzyme RNase H and replaced with DNA by DNA polymerase. The enzyme ligase then seals the gaps between the Okazaki fragments, creating a continuous DNA strand.
5. Proofreading: DNA polymerase checks for any errors and corrects them to ensure accurate replication.
These steps are carried out by a complex of enzymes and proteins called the replisome, which includes helicase, primase, DNA polymerase, RNase H, and ligase.
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Another form of 3) Non-target binding of drug is --- ---. Many drugs accumulate in tissues at levels higher than --- or --- ---; can --- drug action. Can bind cellular proteins, phospholipids, etc tha
Another form of non-target binding of drugs is known as tissue binding. Many drugs accumulate in tissues at levels higher than in blood or other fluids, which can affect drug action. Tissue binding can occur when drugs bind to cellular proteins, phospholipids, and other components within the tissue.
Tissue binding can result in altered drug distribution and elimination, as well as potential toxicity. It is important to consider tissue binding when designing and administering drugs in order to optimize their therapeutic effects and minimize potential adverse effects. Understanding the mechanisms of tissue binding and how they affect drug action is an important aspect of drug development and can help to optimize dosing and minimize toxicity. It is also important to consider the potential for tissue binding when selecting drugs for particular indications and patient populations.
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A student is investigating the process of mitosis with a microscope. The hazard warning label on the chromosome stain they use states: may cause skin irritation, may cause eye irritation and may be harmful if swallowed
Suggest three safety precautions the student should take.
Three safety (3) precautions the student should take:
Safety Precaution 1:
The student should wear gloves to prevent skin irritation from the chromosome stain.
Safety Precaution 2:
The student should wear safety goggles to protect their eyes from irritation caused by the chromosome stain.
Safety Precaution 3:
The student should avoid eating or drinking in the lab to prevent accidentally ingesting the chromosome stain, which could be harmful.
About safety precautions in labLaboratory safety precautions help prevent or avoid accidents and advise what to do in an emergency. As the Boy Scout motto says: Be prepared.
Lists of rules and procedures are common tools for explaining laboratory safety measures. They should be taught to all employees before they start work and reviewed regularly during short refresher days such as safety moments.
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explain obesity problem and how
to solve it.
tell us more about the
problem
what will solve the problem
what supports the solution
Obesity is a medical condition in which excess body fat accumulates to the point of negative health impacts. It is typically caused by an unhealthy diet and lack of physical activity. To solve the problem of obesity, a combination of healthy diet, regular physical activity, and lifestyle changes is needed.
A healthy diet includes eating whole grains, lean proteins, fruits, vegetables, and healthy fats. Regular physical activity can include any type of physical activity such as walking, running, cycling, or swimming. Making lifestyle changes such as reducing stress levels and getting adequate sleep can also help.
Support for these solutions come from studies showing that these measures can help reduce and even reverse obesity. For example, the Centers for Disease Control and Prevention (CDC) recommends an individualized approach to losing weight that includes a combination of healthy eating, physical activity, and lifestyle changes.
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In Maine, a decrease in the gene pool was observed when disease killed off a large portion of the moose population. This is an illustration of
A. Natural selection
B. Genetic bottleneck
C. The Founder Effect
D. Genetic isolation
E. All of the above are correct
The situation stated in the problem illustrates genetic bottleneck. The correct answer is B. Genetic bottleneck.
A genetic bottleneck occurs when a population experiences a drastic reduction in size, leading to a decrease in genetic diversity. This can happen due to natural disasters, disease, or human activities. In the case of the moose population in Maine, the disease that killed off a large portion of the population caused a genetic bottleneck, leading to a decrease in the gene pool.
Natural selection (A) is the process by which certain traits become more or less common in a population due to their impact on an organism's ability to survive and reproduce. The Founder Effect (C) occurs when a small group of individuals establishes a new population, leading to a decrease in genetic diversity. Genetic isolation (D) occurs when a population becomes separated from other populations, preventing gene flow. While all of these processes can impact genetic diversity, the specific scenario described in the question is an example of a genetic bottleneck.
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Three patterns of genetic diversity were seen, a loss in variability, a gain in variability, or an insignificant change in variability. Describe how selection could lead to each of those patterns in evolution.
Selection is the process of choosing individuals that are better adapted to their environment to produce offspring. This can lead to different patterns of genetic diversity in a population.
1. Loss in variability: This can occur when there is strong selection for a particular trait or allele. If one allele or trait is strongly favored, then individuals with that trait will be more likely to survive and reproduce, leading to a decrease in genetic diversity as the favored allele becomes more common in the population.
2. Gain in variability: This can occur when there is selection for diversity, such as in the case of balancing selection. Balancing selection occurs when there are two or more alleles that are favored in different environments or situations. This can lead to an increase in genetic diversity as different alleles are favored in different situations.
3. Insignificant change in variability: This can occur when there is no strong selection for or against any particular trait or allele. In this case, the genetic diversity of the population will remain relatively constant over time.
In summary, selection can lead to different patterns of genetic diversity depending on the strength and direction of selection. Strong selection for a particular trait can lead to a loss in variability, while selection for diversity can lead to a gain in variability. If there is no strong selection, then the genetic diversity of the population will remain relatively constant.
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What is the enzymatic activity of DNA polymerase.
Draw the synthesis of both strands of DNA, show the role of DNA A (ori-binding), DNA B
(Helicase), FIS, histone, HU, IHF, SSB, Gyrase (topoisomerase), Dam (Ori GATC methylase)
The enzymatic activity of DNA polymerase is the process by which a strand of DNA is replicated and/or repaired.
DNA polymerase adds nucleotides, which are the building blocks of DNA, to a single-stranded DNA template in a process known as polymerization. The synthesis of both strands of DNA involves several enzymes, including DNA A (ori-binding), DNA B (helicase), FIS, histone, HU, IHF, SSB, Gyrase (topoisomerase), and Dam (Ori GATC methylase).
DNA A binds to the origin of replication (ori) and opens up the double helix to allow access to the strands of DNA. DNA B, a helicase, then unwinds the DNA and separates the two strands of the double helix. FIS, histone, HU, IHF, and SSB all help maintain the stability of the open single-stranded DNA.
Gyrase (topoisomerase) helps relieve the torsional stress that results from the unwinding of the double helix, and Dam (Ori GATC methylase) helps protect the replication machinery from damage. Finally, DNA polymerase adds nucleotides to the template strands to synthesize the new strands of DNA.
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What are three factors that are essential for the optimal
functional lay out of a cell culture room.
The most efficient and effective design of a cell culture chamber depends on a number of elements. Here are the three most crucial considerations:
Cell cultures must be kept clean to avoid contamination. To avoid cross-contamination, the room should have smooth, easy-to-clean surfaces and designated work areas.
Cell cultures need carefully controlled temperature and humidity. This requires a reliable HVAC system, humidifiers, and regular temperature and humidity monitoring.
Cell culture rooms should be organized for efficient workflow and easy access to equipment and supplies. This means there should be designated areas for different types of work, and the layout should be designed with researchers in mind.
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this could occur with hypoxia, intravenous infusion, ventilation, or pneumothorax (lung collapse), capillary rupture could occur; brain anoxia then occurs distal to the rupture.
It is important to understand the potential causes of capillary rupture and the effects that they can have on the body. By understanding these factors, we can better identify and treat potential issues before they lead to serious complications.
Hypoxia, intravenous infusion, ventilation, and pneumothorax (lung collapse) are all potential causes of capillary rupture. When a capillary ruptures, it can lead to brain anoxia, which is a lack of oxygen in the brain. This can occur distal to the rupture, meaning that the area of the brain that is affected is located further away from the site of the rupture.
It is important to note that each of these potential causes of capillary rupture can have different effects on the body. For example, hypoxia can lead to a decrease in oxygen levels in the blood, which can in turn lead to brain anoxia. Intravenous infusion can cause an increase in blood volume, which can put pressure on the capillaries and potentially lead to rupture. Ventilation can also affect capillary rupture by changing the pressure within the lungs, which can affect the blood flow to the brain. Finally, pneumothorax (lung collapse) can lead to a decrease in oxygen levels in the blood, which can also lead to brain anoxia.
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The Graduate Record Examination (GRE) consists of three sections: Verbal Reasoning, Quantitative Reasoning, and Analytical Writing. The mean score of all test takers for the Verbal Reasoning section was 151 with a standard deviation of 8.66. The mean score of all test takers for the Quantitative Reasoning section was 153 with a standard deviation of 8.09. Suppose that the distributions of both section scores are approximately normal. What is the probability that a randomly selected GRE participant would have a Verbal Reasoning score between 135 and 145?
The probability that a randomly selected GRE participant would have a Verbal Reasoning score between 135 and 145 is approximately 0.2129, or 21.29%.
To find the probability that a randomly selected GRE participant would have a Verbal Reasoning score between 135 and 145, we need to use the z-score formula:
z = (x - μ) / σWhere x is the score, μ is the mean, and σ is the standard deviation.
First, we will find the z-score for a score of 135:
Next, we will find the z-score for a score of 145:
z = (145 - 151) / 8.66Now, we will use a z-table to find the probability that a randomly selected GRE participant would have a Verbal Reasoning score between these two z-scores. The probability that a randomly selected GRE participant would have a Verbal Reasoning score less than -1.85 is 0.0322, and the probability that a randomly selected GRE participant would have a Verbal Reasoning score less than -0.69 is 0.2451.
Therefore, the probability that a randomly selected GRE participant would have a Verbal Reasoning score between 135 and 145 is:
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Which would provide evidence of the history of life from earlier geologic periods?
A. adaptation
B. decomposer
C.fossil
D.predator
NEED HELP ASAP
Answer: C. Fossil
Explanation:
Fossils are impressions of dead organisms that have formed into rock. Studying the location of the fossil allows scientists to date them and understand life in earlier geologic periods.
Even though considered, a light hazard, a residential room could easily have___to___of potential peak HRR, provide sufficient oxygen and ventilation is available.
Even though considered a light hazard, a residential room could easily have 1,000 to 2,000 BTU/ft2 of potential peak Heat Release Rate (HRR), provided sufficient oxygen and ventilation is available.
This is because residential rooms typically contain a variety of materials that can serve as fuel sources, such as furniture, curtains, and bedding. Additionally, residential rooms often have a significant amount of air available to support combustion, due to the presence of doors, windows, and other openings.
It is important to note that the potential peak HRR of a residential room is dependent on a number of factors, including the size of the room, the types of materials present, and the availability of oxygen and ventilation. As a result, the potential peak HRR of a residential room can vary significantly from one room to another.
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Please read this queation and help me to underrestand very well.
Please don't send me other expert's explanations.
in the case of double strand breaks, describe process repair for
this damage?
In the case of double-strand breaks, the process of repair for this damage is called Homologous Recombination (HR).
HR is a mechanism of DNA repair by which two DNA molecules exchange information, producing new recombinant DNA molecules that are not present in either the original or parental molecule. Homologous recombination repairs double-stranded DNA breaks, allowing the broken strand to rejoin the intact, homologous region of another chromosome or the sister chromatid. Homologous recombination involves the use of a template from a homologous chromosome to repair a breakage in the DNA.
It includes the resection of 5′ ends, strand invasion, and heteroduplex DNA formation, DNA synthesis, and resolution. The recombinational repair of DSBs can also bring about genome rearrangements, such as translocations, deletions, and inversions, in addition to restoration of the original structure. So, Homologous recombination plays a key role in maintaining genome stability by repairing double-stranded DNA breaks.
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1. Define the role of the following enzymes; RAG-1 and RAG-2, TdT, and AID
2. Describe how naïve B cells can coexpress IgM and IgD
3. Describe how a B cells switch from producing a membrane bound BCR to a soluble antibody after
antigen encounter
1. RAG-1 and RAG-2 (Recombination Activating Genes) are responsible for initiating V(D)J recombination, which is the process of rearranging DNA to produce a functional antibody.
2. Naïve B cells produce membrane bound immunoglobulins (IgM and IgD) which are involved in the recognition of antigens, and enable activation of the B cells.
3. After antigen encounter, B cells differentiate and switch to producing soluble antibodies.
TdT (Terminal deoxynucleotidyl transferase) is an enzyme responsible for random insertion of nucleotides at recombination joints to create diversity. AID (Activation Induced Deaminase) is an enzyme responsible for deamination of cytosines to generate mutations, which increases antigen binding specificity.
This switch is initiated by the enzyme AID, which deaminates cytosines in the variable regions of the immunoglobulins to produce mutations, which results in higher affinity for the antigen.
The newly produced B cells secrete immunoglobulins, which are capable of binding to antigens with higher specificity and efficiency.
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