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Discuss the use of gel electrophoresis for the separation of macromolecules (DNA, RNA and protein) of different sizes and topological forms. (Include in you answer the different recombinant DNA techniques that require gel electrophoresis) What is the product of -2x^3+x-5 and x^3-3x-4?(A) Show your work.(B) Is the product equal to (x^3-3x-4)*(-2x^3+x-5)? Explain your answer. I NEED HELP PLS I NEED THIS BY FRIDAY t Marie's Beading Boutique, 32 out of the 32 beads on clearance are plastic. What percentage of beads on clearance are plastic?Write your answer using a percent sign (%). What "developing idea of freedom" contributed to the arguments underpinning the Peasants' Revolt? In what ways did medieval religious teachings add to that argument? Explain citing textual evidence. Select all of the characteristics common to both prokaryotic and eukaryotic cells nucleus cytoplasm genetic material membrane bound organelles pls helpWhich evidence from the passage best supports the author's statement that "the court was under many difficulties concerning me"? A. emperor gave orders to certain proper officers to search me B. not feeding me would not rid them of me C. for he expressed himself very imperfectly D. my diet would be very expensive, and might cause a famine 10. Explain which strategy from the two previous questions is the most important, and why. 11. Why is it sometimes necessary to change leaders to ensure cultural change? Answer the basic 5W questions about the American Heart Association study of kids' "screen-time" in Montreal, Canada Both British and Spanish American colonies might have had rebellions to separate from their mother countries in the 18th century, yet it only happened at this point in North America. Why? What was unique about the British colonies and not present in the Spanish colonies? How did these factors influence the potential for revolution at this point in time?write in paragraph form please. Phenol, C6H5OH, is a weak organic acid. Suppose 0. 505 g of the compound is dissolved in enough water to make 125 mL of solution. The resulting solution is titrated with 0. 123 M NaOH. C6H5OH(aq) + OH-(aq) -> C6H5O-(aq) + H2O(epsilon) What are the concentrations of all of the following ions at the equivalence point: Na+, H3O+, OH-, and C6H5O-? Ka = 1. 3 times 10^-10 Lisa is 12(4)/(5) years old. Bill is 1(1)/(6) years older than Lisa and Jane is 1(1)/(3) years older than BilL How old is Jane? Find the area of a regular 8 sides polygon with radius 4 units long. What are important things to think about as we read Eleanor and Park? Factor the following polynomial given that it has a zero at 10 with multiplicity 2 (x^(4)-7x^(3)-118x^(2)+460x+4200) The graph shows the number of birdhouses Penn and his father can build if they have enough time to build no more than 10 birdhouses. What is the domain of this graph? As we start to lose species in an ecosystem, what will happen if the Portfolio Effect is true?a) Nothing at first, but as species with similar ecosystem functions are extirpated, the ecosystem will lose those functions as well.b) A 1:1 decline in ecosystem function per species lostc) If the extirpated species are especially important to ecosystem function, we will lose ecosystem functiond) If the extirpated species dont hold important roles in the ecosystem, it doesnt matter if we lose them. Given matrices =[10]X=[ 10 ] and =[21]Y=[ 21 ] , which of the following matrices is XY ? Now the area can be calculated as: [(7)/(3)t^((3)/(2))-(1)/(5)t^((5)/(2))]_(0)^(7) Read the excerpt from "HuskyThe Wolf-Dog of the North."A peculiarity they are remarkable for is their howling. It can be likened to nothing on land or sea. When the frost grows bitter and the aurora-borealis trails its cold fires across the heavens, they voice their misery to the night. Heartbreaking, sobbing, it rises like a wail of lost and tortured souls, and when a thousand huskies are in full chorus it is as though the roof had tumbled in and (Where Seyton Lives) stood (Not Wearing Cloths) to the stars. No man can hear this for the first time and preserve the equanimity of that portion of his skin which lies contiguous to his spinal column.What is the central idea of this excerpt?A. Huskies often howl when in large groups.B. Huskies howls are not like the howls of wolves.C. Huskies' howls can bring on strong feelings in people.D. Huskies express a wide range of feelings when they howl.