Determine the correct sequence of events during the creation of recombinant DNA.
I. Plasmid is cut open with enzymes
II. Target gene is cut out with enzymes
III. Plasmid is inserted into the bacterium
IV. Target gene is bonded to the plasmid with Ligase
A. IV, I, II, III
B. II, I, IV, III
C. I, II, III, IV
D. IV, III, I, II

Answers

Answer 1

Answer:

I believe the correct answer to your question is A

IV, I, II, III


Related Questions

Which of the following best describes passive transport?
A. Cellular transport that requires cellular energy in the form of ATP.
B. Cellular transport that does not require cellular energy.
C. Cellular transport in which the cell membrane pinches around a substance in order to bring it into the cell.
D. Cellular transport in which substances move from areas of low concentration to areas of higher concentration.

Answers

Answer:

d

Explanation:

passive transport needs no energy W when transporting from high to low and need energy when transporting from low to high

Cellular transport that does not require cellular energy is the example of passive transport. The correct option is B.

What is passive transport?

Passive transport is a type of membrane transport in which substances are moved across cell membranes without the use of energy.

Differing from active transport, the one that uses cellular energy to move substances across cell membranes, passive transport utilizes the second law of thermodynamics to move substances across cell membranes.

Passive transport processes play critical roles in homeostasis. They maintain normal conditions within the cell and the organism as a whole by allowing substances to move into and out of the cell.

Active transport is defined as the movement of a solute from one side of the cell membrane with a low electrochemical potential to the opposite side with a higher electrochemical potential.

Thus, the correct option is B.

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What impact does altitude have on pressure?

Answers

Answer:

As altitude increases, the amount of gas molecules in the air decreases—the air becomes less dense than air nearer to sea level.

Explanation:

Thin air exerts less pressure than air at a lower altitude.

Which of the following is TRUE regarding the flow of blood through the heart:__________
a) The pulmonary artery carries deoxygenated blood.
b) The aorta is responsible for carrying oxygenated blood to the pulmonary system.
c) During systole, blood is going from the atria to the ventricles by gravity.
d) During diastole, blood is flowing over the semilunar valves

Answers

Answer:

a The pulmonary artery carries deoxygenated blood.

Explanation:

This is the the only artery that transports deoxygented blood in mammals. specifically it carry deoxgenated blood from the the atria  to the pulmonary circulation. That is to the lungs  for the exchange of the C02 in the deoxygenated blood with oxygen  in the lungs.

The  blood thus become oxygenated,and therefore return through the pulmonary veins to the left ventricle.

From the left ventricle ,through ventricular systole,blood is empty into the aorta through the aortic valves into the  systemic circulation.

Although,they carry blood away from the heart,but the blood is de oxygenated instead of the usual oxygenated by arteries,They are still regarded as arteries  because of convey of blood away from the heart,a typical role of arteries

Between each presynaptic and postsynaptic neuron is a ____________ that is the fluid-filled space between them. Each presynaptic neuron releases ____________ that binds with receptors in the ____________ segment of a postsynaptic neuron. The ____________ channels of the receptive segment open when these molecules bind. Specific ions then cross the membrane, initiating a ____________ potential in the postsynaptic neuron. This local potential is a small voltage change in the ____________ potential. Graded potentials that occur in postsynaptic neurons are specifically called ____________ potentials.

Answers

Answer:

The correct terms or fillers are -

1. Synaptic cleft

2.Neurotransmitter

3. Postsynaptic

4. Gated

5. Graded

6. Resting membrane

7. Receptive

Explanation:

Between each presynaptic and postsynaptic neuron is a Synaptic cleft  that is the fluid-filled space between them. Each presynaptic neuron releases Neurotransmitter  that binds with receptors in the Postsynaptic  segment of a postsynaptic neuron. The Gated  channels of the receptive segment open when these molecules bind. Specific ions then cross the membrane, initiating a Graded potential in the postsynaptic neuron. This local potential is a small voltage change in the Resting membrane  potential.

Graded potentials that occur in postsynaptic neurons are specifically called  Receptive potentials.

The pathogenesis of pneumonia includes the inhalation of microbial agents and the resulting of which of the following? (Select all that apply.) Inflammation Lung consolidation Formation of exudates Alveolar wall thickening Decreased lung compliance

Answers

Answer:

Inflammation

Lung consolidation

Formation of exudates

Explanation:

Pneumonia could be caused by Bacteria, viruses, and fungi. The air sacs are usually affected and characterized by presence of pus in the sacs. It could be serious and life threatening and can be treated with antibiotics.

The pathogenesis of pneumonia includes the inhalation of microbial agents, Inflammation , Lung consolidation and Formation of exudates.

The protein RDA for adults is 0.8 grams per kilogram of healthy body weight per day. A woman weighs 60 kg and her weight falls within the normal range for her height. How much protein (in grams) should she consume in one day?

Answers

Answer:

Amount of protein required = 48 gram

Explanation:

Given:

Adult required = 0.8 gram protein per kg

Weight of women = 60 Kg

Find:

Amount of protein required

Computation:

⇒ Amount of protein required = Total body weight × 0.8 gram protein

⇒ Amount of protein required = 60 × 0.8

Amount of protein required = 48 gram

Which type of current flows in only one direction and is produced by a battery? magnetic voltage alternating direct

Answers

Answer:

Direct

Explanation:

Direct current flows in one direction are often referred to as unidirectional (uni- one, directional -direction). Whereas, other current flows typically can reverse the flow from time to time which is present in case of Alternating current flow

Direct

Current FlowThe pace at which electrons flow past a location in a complete electrical circuit is defined as current.The flow of electric charges with respect to time is referred to as current flow. When an electric charge flows in one way in an electric circuit, the current flows in the opposite direction.Direct current flows in only one direction and is produced by a battery.

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The sudden rapid movement of an unsaturated cohesive mass of soil, regolith, or bedrock is a The sudden rapid movement of an unsaturated cohesive mass of soil, regolith, or bedrock is a:______
a) debris avalanche.
b) mudflow.
c) landslide.
d) rockfall.

Answers

Answer:

The correct option is C: landslide

Explanation:

A landslide is a huge amount of land, rocks, and other materials that moves downhill. These land moves are caused by erosion and other strengths that may cause the land to move and fall from a steep hillside. Erosion might cause separation of different material layers, and when a land o rocky layer is separated from its immediately anterior one, gravity causes the fall of these layers.

In general, these land moves are very destructive. They can cover rivers, routs, buildings, among others, putting in danger any kind of life.  

There are other moves very similar to landslides, such as mudflow, avalanche, or rockfall.

Rockfall refers to the fall or big rocks from a precipice or steep hillside.

Mudflow refers to a thick stream of mud and other materials.

Avalanche refers to the collapse of snow and other materials.

Read the text: Trans fat is obtained industrially after certain vegetable oils, under certain conditions, are hydrogenated and transformed into solid fat. This type of fat has been used in the production of some food products, such as stuffed cookies, giving them, for example, a more crunchy consistency. However, it is admitted that the excessive consumption of trans fat contributes to obesity and the development of cardiovascular diseases. a) What group of lipids do oils and fats belong to? b) How can we differentiate oils from fats at room temperatures, around 20 ° C? c) Many seeds, such as soybeans, store relatively large amounts of oil. What is the importance of the presence of oil for the embryo of oil seeds? d) Birds and mammals store fats in adipose tissue, under the skin. What is the advantage of the fatty layer under the skin being especially developed in animals that live in cold climates, such as polar bears? e) Recent studies indicate that fat is associated with cardiovascular disease. Name another substance in the lipid group also commonly associated with these diseases.

Answers

Answer and Explanation:

a) What group of lipids do oils and fats belong to? Lipids and fats belong to the triglyceride group. Triglycerides are a mixture of glyceride esters and fatty acids. They are stored in specialized adipose cells, known as adipocytes. Adipocytes give a place to the adipose tissue in the organism.  

b) How can we differentiate oils from fats at room temperatures, around 20 ° C? At room temperature, fats can be recognized for being solid, while oil is liquid.

c) Many seeds, such as soybeans, store relatively large amounts of oil. What is the importance of the presence of oil for the embryo of oil seeds? The plant´s embryo needs to nurture while it is developing. The oil in the seeds plays the role of nutritive storage, together with other nutritive substances. These are used by the plant until it is able to photosynthesize.

d) Birds and mammals store fats in adipose tissue, under the skin. What is the advantage of the fatty layer under the skin being especially developed in animals that live in cold climates, such as polar bears? The advantage of the fatty layer under the skin is to keep or maintain the animal´s temperature. It is very common to find a thick fatty layer under the skin of animals that live in very cold environments, such as polar bears or deep divers marine mammals.

e) Recent studies indicate that fat is associated with cardiovascular disease. Name another substance in the lipid group also commonly associated with these diseases. Cholesterol is a fatty substance needed for the good functioning of the organism. It is part of cellular layers and it plays a role in hormone synthesis and biliary salts. Although in excess they might be harmful as they tend to accumulate in the artery´s walls, blocking the passage of blood. This can lead to a heart attack.

Question 7 of 18
What is the standard deviation of this data set? Use a calculator to help you.
Round your answer to the nearest tenth.
11, 16, 22, 28, 29, 30, 31, 34, 37, 38, 43, 49, 56, 78, 99
O A. 25.9
O B. 23.2
O C. 29.3
O D. 27.5
PREVIOUS

Answers

Answer:

23.2

Explanation:

Corn oil has a density of 0.7 g/cm3. Water has a density of 1.0 g/cm3. A piece of plastic with a density of 0.9 g/cm3 will _____.


sink in corn oil and in water

sink in corn oil and float to the top of water

float in corn oil and in water

sink in corn oil and float in the middle of water

Answers

Answer:

Corn oil has a density of 0.7 g/cm3. Water has a density of 1.0 g/cm3. A piece of plastic with a density of 0.9 g/cm3 will sink in corn oil and float to the top of the water.

Explanation:

Density is a unit that let us know the quantity of mass that a substance has per unit of volume. In a solution, the component with lower density will float, and be on the surface.

If we compare the densities of corn oil and a piece of plastic, the plastic will sink because its density ( 0.9 g/cm³ )is higher than the one of the corn oil (0.7 g/cm³).

In the case of the water, the plastic will float to the top because its density ( 0.9 g/cm³) is lower than the one of water (1.0 g/cm³).

Explain the basic steps in the first half of photosynthesis. Describe the movement of electrons and the importance of hydrogen ions in developing a proton gradient that allows for ATP production.

Answers

Answer:

This simply refers to photolysis of water.

Explanation

Generally,two steps are involved in photosynthesis.

The light  dependent stage or light reaction,(which is the first part of photosynthesis,

The light independent stage (which is the second part.)

The light independent reactions involved the trapping of sunlight by the photo systems, in the leaves,and the splitting of the water molecules by sunlight.

          H20 + Sunlight → 1/2H+ +2 e- +1/2 O2.

The  movement of electron can be cyclic or non cyclic photophosphorylation, In Cyclic, photo system 1 absorbs  the electrons and pass these to the primary pigments in the leaves. This excited the electron  in chlorophyll molecule to higher energy levels , The electron emitted from the leaves is by a process called by photoactivation The emitted electron do not return to the photosystems,rather they are pick up by the primary electron acceptors  and returned to the chlorophyll molecules by electron carriers,NADP.

During this process Energy is released for ATPase synthase of  ATP for phosphorylation of ADP with phosphate,

In Non-cyclic photophorylation,photosystem I and II  absorbs the electrons as Z-scheme  movement of electrons.,Photoactivation  of chlorophyll takes place as in the Cyclic.The primary electron acceptors  takes the  excited electrons  to move along the chain of electron carriers.This makes the photosystems positively charged.

Hence to regain stability the primary pigment of photo system 1 takes electrons from photosystem II.To replace its lost electrons, P II takes electrons from the photolysis of water.ATP is generated  as the carriers molecules transport electrons from the energy produced.

The hydrogen ions is used for the production of electrochemical gradients,which supplies the energy used by the ATPase synthase for the ATPs production.The process is called chemiosmosis. It  involved the  transports of H atoms by NADPH into the stroma,and the splitting of these into protons and electrons,The electrons  form ETC,which forms the PMF.The latter  pumped protons  into the thylakoid membrane,and as this returns it  generate the electrochemical gradient.

The ATP is used in the Calvin Cycle for CHO,proteins productions,

Where do I can find a white deer in far cry primal

Answers

Try to find a flock of deers and look for a golden trace around them. If you see it follow it and it will lead you to a rare white deer or rare animal

what faution generator????​

Answers

Answer:

Fusion power is a proposed form of power generation that would generate electricity by using heat from nuclear fusion reactions. In a fusion process, two lighter atomic nuclei combine to form a heavier nucleus, while releasing energy. Devices designed to harness this energy are known as fusion reactors.

3) a) What are stomata (singular = stoma)?

b) Where on a plant are they located?​

Answers

Answer:

A stoma (singular for stomata) is surrounded by two types of specialized plant cells that differ from other plant epidermal cells. These cells are called guard cells and subsidiary cells.

The majority of stomata are located on the underside of plant leaves reducing their exposure to heat and air current.

Explanation:

Answer:

a stomata is a pore in the coat of the leaves and/or stems

a stomata is found on stems and/or leaves

Dark skin (a result of increased melanin production in equatorial peoples) is likely a response to ultraviolet (UV) radiation, because UV radiation can cause Group of answer choices skin cancer. hypothermia. rickets. hypoxia.

Answers

Answer:

skin cancer

Explanation:

because a human skin system is not evolved to handle the harsh conditions invoked by ultraviolet rays

When the embryo reaches about 500 cells, what best describes its growth and division? A. One daughter cell is a stem cell, one is a tissue cell, they stop being smaller than the parent cell B. Both daughter cells are stem cells at this point and are smaller than the parent cell C. Both daughter cells become specialized cells, they stop being smaller than the parent cell D. One daughter cell is a stem cell, one is a specialized tissue cell, both are smaller than the parent cell

Answers

Answer:

B. Both daughter cells are stem cells at this point and are smaller than the parent cell.

Explanation:

The embryo passes through several consecutive cell divisions. With each division, the daughter cells become smaller in relation to their mother cells. In addition, these cells are embryonic and can be called pluripotent. This is because they have the ability to transform into any type of cell that the human body needs, including tissue cells.

Study the diagram of the geologic time scale. Which method is most likely used to identify what happened before the start of the Paleozoic era?

Answers

Hello. This question is incomplete. The full question is:

Study the diagram of the geologic time scale. Which method is most likely used to identify what happened before the start of the Paleozoic era?

a. Scientists study fossils from the Jurassic period.

b. Scientists observe index fossil records of the Mesozoic era.

c. Scientists examine fossil evidence during the Precambrian time.

d. Scientists explore the emergence of mammals and humans in the Cenozoic era.

Answer:

c. Scientists examine fossil evidence during the Precambrian time.

Explanation:

As you may already know, to facilitate the study of the geological periods of the earth, scientists, especially geologists, decided that the history of the earth should be buried in eras, periods and geological periods, so that the studies could be done in more specific and organized way.

The geological era before the Paleozoic era is the Precambrian period. During this period, there were forms of life on earth that generated fossils that allow scientists to record, date and understand this period, in addition to being able to relate it to others. Thus, the method most likely used to identify what happened before the beginning of the Paleozoic era would be to examine fossil evidence during the Precambrian period.

Answer:

c. Scientists examine fossil evidence during the Precambrian time.

Explanation:

edge 2021

Darwin's Hypothesis of Evolution by Natural Selection included the observation that populations often grow faster than the food supply and therefore individuals need to compete for resources. Darwin borrowed this idea from the work of:

Answers

Answer:

Thomas Malthus on  his essay on population.

Explanation:

Malthus concluded that human population grow faster than the available resources in their environment.However,no matter how this population increases,selection pressure like famine,natural disasters,always  keep the population in check,thus  maintaining the population size within moderate levels.

He believed the weakest in the population (with little resistance to the selection pressure) will die,thereby reducing the population, encouraging the survival of the fittest,and stabilizing and preventing expulsion.

Darwin, assumed that the surviving  organisms in the population must have inherited certain characteristics/traits  from their parents,which make them to resist the selection pressures.He called the surviving ones the survival of the fittest by natural selection.

how does an Amoeba obtain its food​

Answers

Answer:

through the process of endocytosis or phagocytosis,

Answer:

Amoeba Obtain Its Food By

Amoeba envelops its food through the process of endocytosis or phagocytosis, where pseudopodia is created by flexibility of the plasma membrane. ... Amoeba takes in food using temporary finger-like extensions of the cell surface which fuse over the food particle forming a food-vacuole

Explanation:

Good luck ..... Brainliest please

one reason Mendel chose pea plants for his experiment.

Answers

Answer:

Peas were an ideal choice for Mendel to use because they had easily observable traits there were 7 of which he could manipulate

The main reason why Mendel used a pea plant rather than anything else was because a pea plant had observable traits that could be easily recognized. Seven, in particular, he could manipulate in his experiment. Finally, he would cross-pollinate the peas and study each trait that passed on from one pea to another.

Best of Luck!

PLEASE HELP ME I WILL MAKE YOU BRAINLIEST!!!!!!! Need help with number 31!!

Answers

Explanation:

A is 6H2O

B is C6H12O6

C is 6O2

draws conclusions about the nutritional relationships that exist between fox, rabbit and lettuce

Answers

Answer:

Explanation:

According to the nutrition between the fox, the rabbit and the lettuce, it can be concluded:

-All three belong to the food chain of a specific region.

-The lack of any, would make the chain unbalanced, breaking it.

-The fox is a carnivore, so it feeds on the rabbit. While the rabbit is     herbivorous, it feeds on plants.

-The rabbit and the fox are mammals, while the lettuce belongs to the Asteraceae family.

What happens when an antigen is introduced into an organism?
A. Myeloma cells are activated.
B. Monoclonal antibodies are produced from plasma cells
C. Polyclonal antibodies are produced from plasma cells.
D. Polyclonal antibodies are produced from somatic cells.

Answers

Answer:

B. Monoclonal antibodies are produced from plasma cells

Explanation:

An antigen is a molecule present on the surface of a pathogenic organism and they are capable of generating an immune response by binding to specific antibodies (immunoglobulins) that are secreted by lymphocytes B cells of the immune system. B cells are a type of white blood cell that plays key roles during humoral immunity of the adaptive immune system. When an antigen is introduced into the organism, a specific lineage of B cells proliferate, in a process known as monoclonal expansion.

In the late 1870s, Charles Darwin and his son Francis discovered that if the tip of the grass was covered, phototropism did not occur, even though the rest of the grass was exposed to light. However, if the rest of the plant was covered and only the tip exposed to light, the grass bent a few centimeters below the tip. How could light striking the tip of the grass cause bending a few centimeters from the tip

Answers

Answer:

The summary of the particular subject is provided in the segment below on clarification.

Explanation:

Noticed that saplings of the plant would still curve forward into light unless the tip of their ranges were available. The crop developed straight ahead as they cut the edge of such a grass crop, instead of just rotating forward towards the ground.The target stayed straightforward as they put an opaque caps over its end. However, when individuals positioned a transparent caps on its own edge, or perhaps an opaque mask over its core, it streamlined.This was found that perhaps the shoot's tip was essential to detect light. Acknowledged that although the answer to development, trying to bend the shooting, actually happens beneath the tip. A certain message was conveyed downwards from either the service charge to the take the shot's growing market.

The mantle of the moon Select one: a. is composed of two layers. b. may have high levels of iron. c. contains the mineral olivine. d. all of the these.

Answers

Answer:

a. is composed of two layers.

Explanation:

Hope it helps.

Describe 2 facts about organic molecules

Answers

Organic compounds are composed mostly of carbon atoms.

Organic compounds make up life.

Which describes speciation? the adaptation of an extinct species the formation of a new species the extinction of an existing species the grouping of a species

Answers

Answer:

The formation of a new species

Explanation:

My extra notes. Good luck!

Answer:

A and C

Explanation:

100% on edg 2021

which part of a plant serves as a home for nitrogen-fixing bacteria

Answers

Answer:

Mainly the root nodules serve as a home for nitrogen fixation

Explanation:

Egg membranes from three eggs were carefully taken out and labeled as a, b , c. Each of these membranes were filled with 1% solution of sugar. Membrane A was immersed in 0.5% sugar solution, Membrane B was immersed in 1% sugar solution and Membrane c was immersed in 2% sugar solution. Predict the behavior of the three membranes?

Answers

Answer:

The correct answer would be -

Membrane A - Hypotonic solution - the movement of water towards inside the cell

Membrane B - Isotonic soltion - there will be no movement of water

Membrane C - Hyertonic solution - the movement of water towards outside of the cell

Explanation:

This experiment deals with tonicity as this solution will affect the tonicity of the egg membrane. In membrane A there are more solutes inside the cell than outside the cell so it is hypotonic solution so the movement of water will be towards inside the cell.

In membrane B the solutes are equal in both sides so there will be no movement as its isotonic condition while in membrane C the solution is in hypertonic situation as the solutes are more outside than inside.

Thus, the behavior of the membranes are-

Membrane A - Hypotonic solution - the movement of water towards inside the cell

Membrane B - Isotonic soltion - there will be no movement of water

Membrane C - Hyertonic solution - the movement of water towards outside of the cell

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