Describe how restriction enzymes and ligase enzymes are used to make the plasmids that are introduced to bacteria cells during transformation. Include the words sticky ends and complementary in your response.

Answers

Answer 1

Answer:

DNA restriction enzymes cut the DNA molecule, while DNA ligases join the resulting DNA fragments

Explanation:

Transformation is a naturally occurring process by which bacteria incorporate exogenous genetic material from their surrounding environment. This process (transformation) is used for DNA cloning via plasmid vectors. In DNA cloning, transformation occurs after restriction enzymes cut the DNA at specific sequences named palindromic sequences (i.e, sequences that can be read the same in opposite direction). Restriction enzymes can generate sticky-ends, where enzymes make staggered cuts in the two strands (e.g., BamH), or blunt ends, where the resulting strands are of the same length (e.g., HaeIII). In general, sticky-end enzymes are more useful because they generate a 3' overhang in one molecule and a complementary 5' overhang in the other, increasing the yield and specificity of ligation. During ligation, a DNA ligase is used to join both DNA strands by forming phosphodiester bonds in the plasmid. Following transformation, bacteria can be selected on antibiotic plates.


Related Questions

Give the word equation for photosynthesis.
[2 marks]

Answers

Answer:

Carbon dioxide + Water → Glucose + oxygen

The area of the picture highlighted by the green square is a monomer of nucleic acids(DNA/RNA). Which of the following is the name for that monomer?

nucleotide

phosphate backbone

thymine

Answers

Answer:

Examples of the monomers are glucose, vinyl chloride, amino acids, and ethylene. Every monomer can link up to form a variety of polymers in different ways. For example, in glucose, glycosidic bonds that bind sugar monomers to form polymers such as glycogen, starch, and cellulose

Explanation:

Answer:

Nucleotide

Explanation:

What is an example of a physical weathering?


A: a metal tool left outside beginning to rust
B: microscopic organisms producing compounds that break down rock
C: a rock wearing down due to acid rain
D: bits being blown off a rock by heavy winds

Answers

Answer:

D) Bits being blown off a rock by heavy winds

Explanation:

All the other answer choices are examples of chemical weathering.

Rusting is chemical weathering.

Compounds that break down rocks is chemical weathering.

Acid rain is chemical weathering.

Wind breaking rocks, however, is physical weathering.

What is the difference between a normal gene sequence and a mutated gene sequence?

Answers

Answer:

Is that original sequences do not have any mutated nucleotides or damages in DNA whereas mutated sequences may either contain nucleotide alterations or DNA damages

Explanation:

HELP I ONLY HAVE 3 MINUTES If a species of animal was exposed to a deadly virus, which reproductive strategy would give the species a higher chance to survive?

a.neither
b.both are the same
c.sexual reproduction
d.asexual reproduction

Answers

Actually, it is C. In this way, a mutation can occur to make that organism able to survive the virus.

3. The right sided of the heart is completely separated from the left side by a wall of tissue called the _______________.

Answers

Answer: Septum

Explanation:

In the normal heart, the right and left chambers are completely separated from each other by a wall of muscle called a septum. The right atrium is separated from the left atrium by the atrial septum and the right ventricle is separated from the left ventricle by the ventricular septum.

:)

Explain why the earth is flat. Use two reasons in your answer [2 marks]
1.
2.

Answers

Answer:

Reasons why the earth is flat:

1. The earth is not flat

2. The earth is round  

You need to change schools <3

what is the biological role of hemoglobin? (giving brainly for a well structured explanation)

Answers

Answer:

Hemoglobin is essential for transferring oxygen in your blood from the lungs to the tissues. Myoglobin, in muscle cells, accepts, stores, transports and releases oxygen.

Explanation:

hope this help:)

Answer:

the answer is b I got a 100

what is the definition of stimulus and response​

Answers

Explanation:

The stimulus–response model is a characterization of a statistical unit. The model allows the prediction of a quantitative response to a quantitative stimulus, for example one administered by a researcher.☺️☺️☺️

an act of responding. 2 : something constituting a reply or a reaction: such as. a : a verse, phrase, or word sung or said by the people or choir after or in reply to the officiant in a liturgical service.☺️☺️☺️follow me

A couple has six children, all with blood type A. The mother’s blood type is O, and the father blood type is A. Based on this information, which describes the most probable genotype of the father?

Answers

Answer:

most probable bg of father is IAIA

Identify the levels of biological classification and what is different about each.

Answers

Cells —-> Tissue
Tissues —-> Organ
Organs —> organ system
Organ system—-> organism
Organisms —-> Population
Populations —-> Community
Communities —-> Ecosystem
Ecosystems ——> Biomes
Biomes —-> Biosphere (aka Earth).

why does water take up more space than cold water? Your explanation should include the behavior of molecules in the water. 
HELP ASAP!!!!!!

Answers

Explanation:

the molecules in water is more tightly packed than ice, so water has greater density than ice as water freezes it expands. so ice has more volume ( it takes up more space, but has less density) than water.

One of the greenhouse gases, methane has a particular molecular structure that enables it to trap more heat than carbon dioxide However, methane does not exist as long as carbon dioxide in the atmosphere: both naturally and as a result of man's activities on earth . Identify all the sources of naturally occurring methane

Answers

Answer: Wetlands, animal digestion, along with thermogenic sources such as oil and gas production.

Explanation:

Methane (CH4)

Methane comes from natural sources, such as wetlands and animal digestion, along with thermogenic sources, including oil and gas production. Natural gas is approximately 90% methane. Hope this helped!

Answer: A) V and VI

Explanation: got it right on USATP

Recovery Where are the majority of chloroplasts found? A. in animal cells B. in prokaryotes C. in rose petals D. in the leaves of plants​

Answers

Answer:

D

Explanation:

In plants, chloroplasts occur in all green tissues, though they are concentrated particularly in the parenchyma cells of the leaf mesophyll.

Sodium has one electron in its outer energy level. According to the octet rule, _____.

Answers

Answer:

Referring to the octet rule, atoms attempt to get a noble gas electron configuration, which is eight valence electrons. Sodium has one valence electron, so giving it up would result in the same electron configuration as neon. Chlorine has seven valence electrons, so if it takes one it will have eight (an octet).

it wants to give up an electron?

Explanation:

if this helped could i have a brainlist

An investment in labor helps a business increases productivity by

Answers

Answer: Providing more tools to make goods more quickly, thereby increasing the output.

Where is the intertidal zone located?
A. where the ocean meets the shore
B. where the ocean floor starts to slope downward
C. the ocean floor
D. where the ocean floor drops sharply

Answers

Answer:

its where the ocean meets high and low tide sooooo I'd say A? sorry if I'm wrong


Which of the following is a possible base pair?
АТ
GA
ст
АВ


WILL MARK BRAINLIEST

Answers

GCAT is a good way to remember this. G can only pair with C and A can only pair with T.

The possible pairs are either GC or AT.

The answer is AT.


In addition to how much force Earth exerts on the object, which features of an object affect its weight?
mass and location of the object
shape and location of the object
location of the object and how much energy the object has
mass of the object and how much energy the object has

Answers

Answer: A (I got it right)

In addition to how much force Earth exerts on the object, which features of an object affect its weight?

mass and location of the object

shape and location of the object

location of the object and how much energy the object has

mass of the object and how much energy the object has

Explanation:

Answer: A

Explanation:

hope this helps!

A forest is cut down to plant a field of corn.How will this affect the bioversity of the area?

Answers

Answer:

Since the egg membrane is semi-permeable, water can move in but proteins cannot move out. If a naked egg is placed in the corn syrup the egg will shrink. This is also due to osmosis, but in the opposite direction.

Explanation:

Which of the following statements best describes Community Supported Agriculture (CSA)?
A. a service club that provides volunteer labor to local farmers
B. special zoning laws permitting agriculture within residential neighborhoods
C. a local tax on the town or county scale that is contributed to local farmers
D. local farms that sell produce directly to subscribed consumers

Answers

Answer:

D

Explanation:

D. local farms that sell produce directly to subscribed consumers

/Community-supported agriculture (CSA) is a food production and distribution system that directly connects farmers and consumers. In short: people buy "shares" of a farm's harvest in advance and then receive a portion of the crops as they're harvested.

I believe it would be b

If 34 percent of the bases in a DNA molecule are adenines, then which of the
following is true?
O Thirty-four percent of the bases in the molecule will be thymines
Thirty-four percent of the bases n the molecule will be cytosines
Sixty-six percent of the molecule will be deoxyribose sugars.
Sixty-six percent of the molecule will be phosphate.

Answers

Answer:

34 percent will be thymine since the amount of thymine corresponds with the amount of adenine.

Label each organism with its symmetry. Fill in asymmetry, radial, or bilateral.


1.) humans __________

2.) sponges ________

3.) starfish _______

4.) monkey ________

5.) baboons _______

6.) flatworms _______

7.) birds _______

8.) fishes ______

Answers

Answer:

1.) Humans: Bilateral symmetry

2.)sponges: asymmetry

3.) starfish: radial symmetry

4.) monkey: Bilateral symmetry

5.) baboons: Bilateral symmetry

6.) flatworms: Bilateral symmetry

7.) Birds: Bilateral symmetry

8.)fishes: Bilateral symmetry

Explanation:

The divisions and subdivisions of the kingdom Animalia is based on several important features which includes the body symmetry.

Body symmetry is defined as balanced division of body parts in an orderly manner. There are three main types of body symmetry these includes:

--> Asymmetry

--> Bilateral and

--> Radial symmetry

Asymmetrical body division involves animals that when divided, doesn't show any identical features. That is one side doesn't match the other side.. Atypical example is seen in phylum porifera, the sponges. They are simple aquatic invertebrates that lack specialised tissues.

BILATERAL SYMMETRY: This is where the body of animals can be cut along its axis in only one plane to give two identical halves, the right and the left side. The terms anterior, posterior, ventral, and dorsal are used to describe a bilateral symmetrical animal. The evolution of bilateral symmetry allow body parts to become specialised seen in most advanced animals.

RADIAL SYMMETRY: this involves animals where the body can be cut along its axis in any plane to give two identical halves. This is an ideal body symmetry for immobile organisms such as starfish and hydra.

Label each organism with its symmetry:

humans - bilateral symmetrysponges - asymmetrystarfish - radial symmetrymonkey - bilateral symmetrybaboons - bilateral symmetryflatworms - bilateral symmetrybirds - bilateral symmetryfishes - bilateral symmetry

Symmetry

It represents Regularity or a pattern in the arrangements of body parts of an organism. Each organism can be classified by three types of body plan symmetry:

radial symmetry - regularity of parts around a central axisbilateral symmetry - there is a left and a right side along the midlineasymmetry - having a disproportionate arrangement of parts

Learn more about the classification of symmetry:

https://brainly.com/question/238772

What is the approximate temperature of the troposphere, mesosphere, and stratosphere.​

Answers

Answer:

Heat is produced in the process of the formation of Ozone and this heat is responsible for temperature increases from an average -60°F (-51°C) at tropopause to a maximum of about 5°F (-15°C) at the top of the stratosphere. This increase in temperature with height means warmer air is located above cooler air.

Explanation:

Example #2: Oil is poured into a glass water. The oil and water are mixed.

What happens over time?

A) The oil becomes evenly distributed in the water.

B) The oil separates out.

C) The oil chemically combines with the water.

Answers

Answer:

C

Explanation:

im a mechanic i know this stuff

Where are the plates in the convection currents

Answers

Answer:

Convection currents drive the movement of Earth's rigid tectonic plates in the planet's fluid molten mantle. In places where convection currents rise up towards the crust's surface, tectonic plates move away from each other in a process known as seafloor spreading (Fig. 7.21).

Explanation:

3.
We prefer a wire of
energy
for making the 'heating element in
4.
5.
Electromagnet should show rapid
when the current is switched
domestic appliances like the electric iron, electric heater and the electric toaster.
A safety device, based on the heating effect of current is the​

Answers

Answer:

what's your question I am not understanding state the correct question

What is an invasive species and how can it affect an ecosystem?

Answers

An invasive species is an organism that causes ecological or economic harm in a new environment where it is not native. Invasive species are capable of causing extinctions of native plants and animals, reducing biodiversity, competing with native organisms for limited resources, and altering habitats.

What is the number of chromosomes in a haploid cell?

Answers

Answer:

23 chromosomes

Explanation:

In humans, gametes are haploid cells that contain 23 chromosomes, each of which a one of a chromosome pair that exists in diplod cells. The number of chromosomes in a single set is represented as n, which is also called the haploid number. In humans, n = 23.

Answer:

23 chromosomes

Explanation:

In humans, gametes are haploid cells that contain 23 chromosomes, each of which a one of a chromosome pair that exists in diplod cells. The number of chromosomes in a single set is represented as n, which is also called the haploid number. In humans, n = 23.

In human beings, the cell that results from the fusion of an egg cell and sperm cell is considered to be

Answers

Answer:

birth

explanation:

when the sperm comes into contact with the egg the sperm penetrates resulting in a baby

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