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The order for rDNA is to identify a target gene, select a plasmid, use a restriction enzyme, gene insertion, use ligase, introduce the recombinant DNA and detect recombinant cells. rDNA involves genetic recombination.
Recombinant DNA (rDNA) technologies are used in molecular biology laboratories to create chimeric DNA molecules containing nucleotide sequences from different species.
Recombinant DNA techniques can be used to identify and sequence genes, as well as to discover gene function.
The sequential steps of rDNA technologies are as follow:
Identify a gene of interest.Select a DNA plasmid to be used.Use restriction enzymes to cleave the plasmid DNA.Introduce the gene of interest in the plasmid.Use ligase enzymes to join both nucleotide sequences.Introduce the recombinant DNA molecule into the host cell.Detect and replicate the cells containing the desired recombinant DNA molecule.Learn more in:
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Related Questions
Describe a hypothetical result of the fluctuation test that would have supported the hypothesis that mutations occur in response to an environmental challenge, and explain how this result was different from the observed result described above
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In order to support the hypothesis that mutations occur in response to an environmental challenge, the experiment in question would have to show an increase in mutation rates shortly after the environmental challenge was introduced.
When we test a hypothesis, we perform an experiment and gather data over the course of the testing. In order to obtain evidence that supports a hypothesis, we have to ensure that only one variable is changing and be sure to repeat the experiment to confirm the values measured.
Taking this into account, a hypothetical result of the fluctuation test that would support the hypothesis of mutations in reaction to environmental challenges would be to measure a spike in mutation rates in the generation following an environmental challenge, and then continue to do so with future post-challenge generations.
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Which of the following statements is true about body system interactions?
a. When someone drinks fluids, these fluids are absorbed into the blood stream (cardiovascular system) from the small intestines (digestive system).
b. When someone drinks fluids, the kidneys (urinary system) sends a message to the brain (nervous system) that more water needs to be released from the body.
c. When someone drinks fluids, these fluids are absorbed into the kidneys (urinary system) from the stomach (digestive system).
d. When someone drinks fluids, these fluids are transported directly to the kidneys (urinary system) by the blood stream (cardiovascular system) and go nowhere else in the body.
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Answer:
true
false
true
true
hhhh
In granulocytes, what happens to the nucleus as the cell ages?
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The granulocytes have been commonly recognised and distinguished histologically by their nuclear shapes and sizes. They contain multi-lobed nuclei, each lobe connected by a short region of nucleoplasm.
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Granulocytes are recognized and distinguished histologically by their nuclear shapes and sizes.
Do granulocytes have a nucleus?The granulocytes have been commonly recognized and distinguished histologically by their nuclear shapes and sizes. They contain multi-lobed nuclei, each lobe connected by a short region of nucleoplasm.
What do granulocytes turn into?
A granulocyte is a type of white blood cell. A blood stem cell goes through several steps to become a red blood cell, platelet, or white blood cell.
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How waste is removed from plant body ?
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Answer:
Living organisms sustain life, based on certain life processes. These life processes help to regulate their daily activities. Excretion is one among them. The metabolism takes place in the cells of living organisms resulting in both useful and toxic products. The accumulation of toxins may harm the organism. Thus, the living organisms remove all these metabolic wastes from their body and this process is called excretion. Different organisms follow different modes of excretion. Let’s have a glance at excretion in plants and how excretion in plants is different from that of animals.
Explanation:
I hope you can help buddy (:
Answer:
the excretory organ removes these westes.
this process of removal of metabolic waste from the body is known as excretion
why behavioral perspectives is the most logical perspectives in your opinion. Explain
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Answer:
The behavioral approach is the most logical perspective because it is easier to quantify and collect data and information when one is conducting a research.
Explanation:
What are the four things that plants need to live
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Answer:
Sunlight, water, carbon dioxide, dirt/soil
Explanation:
which is NOT a example of a biotic factor?
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Answer:
Explanation:
Plants, trees, humans, insects, animals, birds, etc. ... Abiotic factors are those that contain non-living organisms. These factors include soil, air, water, sunlight, etc.
Question 1: DNA remains in the nucleus. mRNA can travel in and out of the nucleus. Explain how the function of DNA and mRNA differs. ( Will Mark Brainliest and please only answer if your company 100% sure. And Do Not Repost Someone Else's Answer that is on Brainly Or Any Other Websites Please.
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Answer:
DNA is a nucleic acid that carries the genetic instructions but mRNA carries information from the DNA to the cytoplasm where proteins are made
the location where the restriction enzyme “cuts” is called a..
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What does a pyramid of biomass represent? A. Amount of living tissue within a tropic area.
B. Percentage of energy that’s passed through each tropic level.
C. Range of food webs found in one trophies level.
D. Number of total organisms within each trophic level.
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Explanation:
I hope it's helpful
B
Explanation
help now asap plssssss ols dont answer if you dont know
Answers
Answer:
telescope
Explanation:
Answer:
Optical Telescopes , Radio Telescopes , Spectroscopes , Star Charts
Explanation:
how can you prepare a balanced ration for pigs from locally available ingredients ?
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Answer: Feed your pigs that are being weaned off of their mother's milk, a special mix of food until they are about 10 to 12 weeks old. Then create a feed mix that is comprised of 76 percent corn, 12 percent skim milk powder, 6 percent soybean meal, 6 percent calcium and protein, and also 0.2 percent salt.
Explanation:
A program designed to interfere with your computer, device or network's normal operation is called a Answer
Answers
Answer:
A virus
Explanation:
If a mutation occurs such that splicing does not remove one of the introns in a gene, what effect will this have on the protein encoded by that gene
Answers
Translation will continue, but it is likely that a nonfunctional or aberrant protein will be made as a result of this effect.
Mutation is described as the alteration in the genetic sequence caused by varying effects. Diseases and exposure to dangerous materials or elements (like x-rays) can cause mutation.
However, when alteration in a genetic sequence is effected such that the splicing that occurs prior to mRNA translation, does not remove a segment of a DNA or RNA molecule (which does not code for protein), and this will disrupt the gene sequence. Although, translation will continue, but the protein synthesised will be a nonfunctional or abnormal one.
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what you know about meiosis, fertilization, genotypes, and phenotypes to describe how traits are inherited. Include predicted ratios of types of offspring. This is a combination of learning objectives 3.18 (meiosis), 3.23 (fertilization), 4.1 on inheritance, and 4.2 on monohybrid crosses. This will require deep thinking about how these four learning objectives are connected.
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Meiosis is a type of cell division in which four daughter cells are produced from a single parent cell. In each daughter cell, half number of chromosomes are present. This type of cell division occurs in sex cells such as sperm and eggs.
Fertilization is a process in which a sperm cell successfully meets or combine an egg cell. The newly fertilized cell is called zygote is produced during the process of fertilization.
A genotype is a collection of genes. Genotype is also refer to the two alleles that are inherited for a particular gene. The genotype is expressed when the information encoded in the genes' DNA is used to make protein and RNA molecules.
The term "phenotype" refers to the physical properties of an organism which can be seen. Phenotype includes the appearance, development, and behavior of an organism. An organism's phenotype is determined by its genotype.
Meiosis, fertilization, genotypes, and phenotypes are interconnected to each other because all these terms perform a specific contribution in the process of reproduction.
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the business cycle illustrates the long-run fluctuations of ___.
a) real GDP
b) nominal GDP
c) aggregate supply
d) the dollars value
Answers
Answer:
1 Real GDP
2 expansion stage of the business cycle
3 two
4 high unemployment
5 shock
OCA Stages of the Business Cycle Quick Check
Morgan and his fruit flies are famous for two discoveries. What are they and how do they help us in understanding genetics?
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The carbon dioxide that is used by plants ________
1. Is combined with oxygen to make sugar molecules
2. Is absorbed through the roots of plants
3. Comes from the air
4. Is actually a source of food
Answers
Answer:
3
Explanation:
because carbon dioxide can be found in air
Answer:
The answer is 3.) Comes from the air
Explanation:
Since in this question, were talking about carbon dioxide, there is only one place where the plant can take the carbon dioxide. And that one place is the air.
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provide an example of a theory that is a hypothesis supported by repeated experimentation.
Answers
Answer:
A good example of scientific theory repeated many times is the “theory of relativity” proposed by Albert Einstein in 1915 which was tested and repeated in 1919 at the time of solar eclipse which was later repeated again by many scientists.Explanation:
It's a good fact. Hope this helps
The three most common homoglycans found in nature are cellulose, starch and __________. Group of answer choices Amylose Lactose Fructose Glycogen Sucrose
Answers
Answer:
Glycogen
Explanation:
Select all of the muscles that can be involved in abducting or adducting the eye.
Check All That Apply
lateral rectus
medial rectus
superior rectus
inferior rectus
Answers
The lateral rectus and the medial rectus muscles are responsible for Adduction and Abduction of the eye
The muscles of the eye help to move and stabilize both eyes. Six extraocular muscles control all of the movement of the eye which are
Superior rectus, Inferior rectus, lateral rectus, medial rectus, superior oblique, inferior oblique.Adduction is the movement of the eye towards the nose with the medial rectus muscle responsible for it while Abduction is the movement of the eye away from the nose with the lateral rectus responsible for it .
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1. What makes up a phenotype of a plant or animal?
2. What are the genes that produce a phenotype?
3. The study of heredity is called _________________.
4. Who is considered the “father” of genetics?
5. Genes had two copies each. These copies are called ___________.
6. What are Mendel’s 4 findings from his study?
7. The nucleus contains genetic material, or ______. (This is short for _______________________.)
8. How many PAIRS of chromosomes do humans have?
9. True/False: All species have the same number of chromosomes.
10. What are the four kinds of bases that join together in DNA strands?
11. What three people were instrumental in discovering DNA?
12. ___________ _________ make up proteins, and ____________ provide the code to make those proteins.
13. The human genome contains ________________ genes.
14. Describe sequencing.
15. Human DNA is 96% identical to the DNA of ___________________.
16. What are mutations and what do they cause?
17. Adaptations are caused by a ________________ in the DNA of an organism.
18. Describe one way studying genetics of organisms can be beneficial?
19. What are some ethical concerns we have about genetic studies?
Answers
Answer:
#1 The term "phenotype" refers to the observable physical properties of an organism; these include the organism's appearance, development, and behavior. An organism's phenotype is determined by its genotype, which is the set of genes the organism carries, as well as by environmental influences upon these genes.
#2 Phenotype
#3 Genetics
#4 Gregor Mendel
#5 an allele
#6 Gregor Mendel, through his work on pea plants, discovered the fundamental laws of inheritance. He deduced that genes come in pairs and are inherited as distinct units, one from each parent. Mendel tracked the segregation of parental genes and their appearance in the offspring as dominant or recessive traits.
#7 The nucleus (plural, nuclei) houses the cell's genetic material, or DNA, and is also the site of synthesis for ribosomes, the cellular machines that assemble proteins.
Explanation:
Explain the use of oxytocin in labor inductoin in the case of obstructed labor
Answers
When did Lincoln declare that Thanksgiving would be celebrated on the last Thursday of November?
Answers
During the process of photosynthesis,
which of the following is being created
by producers?
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Answer:
Producers are the plants that use sunlight, water, and carbon dioxide to make their own food. They are called producers because they produce sugars from CO2 which they share with the rest of the world through photosynthesis. The carbohydrates, fats, proteins etc in your diet were all originally made by green plants! All green plants need is CO2 and water to live.
Explanation:
Answer:
Sugar and oxygen
Explanation:
energy from the sun converts the carbon dioxide and water it absorbs into sugar and the byproduct of oxygen
The term ____ refers to anatomical structures that are found on the same side of the body.
A. Contramedial
B. Bilateral
C. Contralateral
D. Ipsilateral
Answers
Answer:
D. Ipsilateral
Explanation:
Ipsilateral is the occurrence of anatomical structures like the muscles, body organs, parts, etc., on the same side of the body. Thus, option D best fits the blank.
What is Ipsilateral?Ipsilateral is an anatomical terminology that defines the position, regions, and body structures that lie or occur on the same side of the body. On contrary, contralateral defines the structures on the opposite side of the body.
Bilateral is an anatomical term that refers to the anatomical structures that are present on the left and right side of the body and affects both sides. It defines the symmetry of the body.
Therefore, the Ipsilateral refers to structures of the same side.
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in terms of volume does blood normally contain more plasma or more formed elements
Answers
Answer:
I think blood is made up of more plasma
Explanation:
I think more than 50% is made up of blood plasma
Meiosis occurs in the organs of which body system?
1.) reproductive
2.) nervous
3.) circulatory
4.) muscle
Answers
Answer:
A Reproductive
Explanation:
A Reproductive is the answer.
Can Earth's human population increase indefinitely? *
*
A) yes, even though resources are finite
B) yes, because resources are infinite
C) no, even though resources are infinite
D) no, because resources are finite
Answers
Answer: D
Explanation: Humans need resources to survive, and eventually those resources will not be enough to provide for the population, meaning a population cap.
When babies are born, many
of their bones are still
cartilage. As they grow, these
harden into bone.
What do we call the process
of forming and hardening
bone?
A. digestion
B. circulation
C. ossification
Answers
Answer:
C. ossification
Explanation:
All bones develop in the same way in a process called ossification. At about 10 weeks, bone tissue starts to form as cartilage or membrane. Then, calcium and phosphate – minerals stored in your body and replenished by the foods you eat – are added to the tissue