Based on your knowledge of the genetic tools for studying baker's yeast, how would you clone the genes that are mutated in your respective yeast strains?

Answers

Answer 1

Answer:

By using specific primers for PCR amplification and DNA sequencing

Explanation:

In molecular biology, different techniques are used to clone the full sequence of desired genes. In general, gene cloning is based on the assembly of genetically engineered recombinant DNA cloning vectors into host organisms. The cloning procedure generally consists of isolating target DNA sequences to be cloned, ligation into a corresponding vector, transfection and screening of clones. The recombinant DNA is first amplified by PCR. Subsequently, DNA is sequenced and finally assembled by bioinformatic methods in order to characterize the full sequence of the gene.


Related Questions

While sitting at your desk, you drop your pencil onto the floor. You bend over to pick up the pencil. In order to straighten up and continue your exam you must use which muscles?

Answers

Answer:

Deltoid muscle

Trapezius muscle

Carpel muscle

Quadriceps

Hamstrings

Glutes

Abdominal muscles

what happens when liquid is heated? class five​

Answers

Explanation:

when a liquid is heated it will break . The molecules there will be vibration of molecules and then they will loosen up(the molecules that is) and then there will be a lot of heat which will remain constant and then the heat will make the liquid to evaporate (which is known as boiling point)

1. One important feature of DNA is that it can be accurately copied and inherited from one generation to the next. Imagine if DNA was NOT accurately replicated. What errors could occur and how would they affect the offspring in the next generation

Answers

Answer:

Explanation:

If DNA is not accurately replicated it can lead to mutation. Mutation is a genetic disorder that occur when a change occur and affect the genome of an organism. It can be result in frameshift mutation shifting nucleotide arrangement.

When DNA is not accurately replicated, it could lead to formation of an entirely new protein different from the original which has a different function this could lead to a form of disorder in the offspring. The effect of this disorder depends on it severity.

Describe the biosynthetic pathway that produces catecholamines such as norepinephrine and epinephrine from amino acid precursors and explain how the lack of available amino acids precursors would impact homeostasis regulated by catecholamines.

Answers

Answer:

Tyrosine and phenylalanine are amino acids required for the synthesis of catecholamines

Explanation:

The catecholamines are hormones composed of a catechol ring group (benzene) and an amine lateral chain. One of the most common catecholamines is the neurotransmitter norepinephrine (noradrenaline). The biosynthesis pathway of the norepinephrine hormone includes the following steps: 1-tyrosine hydroxylase produces L-DOPA from tyrosine, 2-L-DOPA is subsequently tranformed into dopamine by the L-amino acid decarboxylase, and finally, 3-this chemical precursor is converted into norepinephrine by the action of the dopamine β-hydroxylase.  

Phenylketonuria is a congenital metabolic disease associated with a decrease in the metabolism of phenylalanine (Phe), which is an amino acid residue that acts as a precursor of different catecholamines including dopamine, norepinephrine (noradrenaline), and epinephrine (adrenaline). Dietary restriction of tyrosine and phenylalanine is the most common treatment against phenylketonuria.

Natural resources are not required for all energy producing technology
Please select the best answer from the choices provided
OT
OF

Answers

Answer:

True

Explanation:

Artificial resources can be used too.

Hope it helps.

Assume a population of deer has a crude birth rate of 36 per 1,000/yr, a crude death rate of 20 per 1,000/yr, an immigration rate of 15 per 1,000/yr, and an emigration rate of 8 per 1,000/yr. What is the population growth rate

Answers

Answer:

0.023%

Explanation:

The Population growth rate is a change in population with time taken into consideration. It can be expressed with the formula

[tex]N/t[/tex] where N is the total change in the population size, and t is time.

When immigration and emigration are factored in, we now have the formula;

[tex](CBR +I) - (CDR + I)/1000[/tex]

[tex]= (36 + 15) - (20 + 8) = 51 - 28= 23\\\\expressing as a percentage of the population gives us,\\\\23/1000=0.023%\\\\[/tex]

Fast reproduction by binary fission enables bacteria to
cause infections in other organisms.
quickly modify their DNA to changing conditions.
change shape from coccus to bacillus.
transfer DNA from one cell to another.

Answers

Answer:

A.) Cause infections in other organisms

Explanation:

Binary fission helps the bacteria to cause infections in other organisms because of duplication of its genetic material.

Through fast reproduction in bacteria enables to cause infections in other organisms by binary fission.

How does binary fission help bacteria reproduce quickly?

During binary fission, the chromosome copies itself, forming two genetically identical copies. Then, the cell enlarges and divides into two new daughter cells.

The two daughter cells are just like the parent cell. Binary fission can happen very rapidly.

In the process of binary fission, an organism duplicates its genetic material, or desoxyribonucleic acid (DNA), then divides into two parts (cytokinesis), with each new organism receiving one copy of DNA.

Many prokaryotic organisms like bacteria reproduce by the method of binary fission.

Therefore, the correct option is A that bacteria cause infections in other organisms.

Learn more about the binary fission here:

https://brainly.com/question/82412

The spread of cancer cells from one site to others in the body is known as _____.​

Answers

Answer:

metastasis

Explanation:

Metastasis is the process in which cancer cells break away from the place where they first formed and travel through the bloodstream to other parts of the body where they form a new tumor.

Hope that helps.


what is a type of emigration where offspring move
away from their parents.

Answers

Answer:Dispersal

Explanation: This refers to offspring moving away from their parents. This prevents the offspring from competing with the parents for resources such as light or water. For example, dandelion seeds have “parachutes.” They allow the wind to carry the seeds far from the parents

A cylinder at left with balls evenly spaced throughout the cylinder has an arrow leading to a cylinder at right cylinder with balls stacked near its bottom. How is energy related to the change of state represented by the model? Atoms gain energy as a gas changes to a solid. Atoms gain energy as a gas changes to a liquid. Atoms lose energy as a gas changes to a solid. Atoms lose energy as a gas changes to a liquid.

Answers

Answer:

C. Atoms lose energy as a gas changes to a solid.

Explanation:

Physical changes of matter take place by adding or removing energy from the matter.

In the given images, The left cylindrical is showing gas phase while the right cylindrical is showing a solid phase.

The process of conversion of gas into solid is called deposition. Deposition process takes place when atoms lose their energy and have high kinetic energy so they directly convert into solid and not into liquid.

Hence, the correct option is "C".

Answer:

c i took the quiz

Explanation:

In melosis, the homologous chromosomes are side by side. Are they side by side in mitosis?

Answers

in metaphase 1 of meiosis, the homologous chromosomes line up side by side along the equator so that crossing over can occur between the homologous pairs and the homologous chromosomes can be pulled to opposite poles

I'm confused on labelling the stages of mitosis in these onion cells. I know what these stages look like illustrated in the textbook, but am confused when looking at actual slides of cells. Can anyone help? Photo is attached.

Answers

Answer:

Hello. I numbered your cells to make it easier to name them. The image with the numbers is attached.

cell 1: Interphase.

Cell 2: Telophase.

Cell 3: Metaphase.

Cell 4: Prophase

Cell 5: Interphase.

Cell 6: Anaphase.

Explanation:

In the interphase, it is possible to notice that the cell increased its volume, due to the increase in the size of the organelles and the increase in the number of the organelles, which indicates the beginning of the cell cycle.

Prophase is really the beginning of the cell cycle. At that moment, you can see that the nuclear membrane has been disorganized and we can see a 'tangle' of the cell interior that is very full and disorganized. This is mainly because the material present in the nucleol spreads through the cell.

In the metaphase, the chromosomes are condensed and start to connect to each other forming the spindle fibers, which are located right in the middle of the cell, forming the equatorial plate.

In anaphase there is a rupture of the centromeres and sister chromatids. Each part of these elements migrates to one of the poles of the cell. This is mainly due to the shortening of the spindle fibers.

Telophase is the final phase of the cell cycle, at that moment, the spindle fibers disappear, the chromosomes are condensed and the nuclear membrane is reconstructed. However, the cell has not yet separated and it is possible to see two nuclei in a single cell, each nucleus is at a pole, indicating that the division will occur.

Which sequence shows the transfer of oxygen for use by the body?

Answers

Answer:

Hello!!

I think your answer is GAS EXCHANGE!

Explanation:

HOPE THIS HELPS!!

itsMATT04

The right answer is D.

Breathing is the process by which oxygen from the air gets into your lungs and gets into your body. Our lungs take oxygen from the air and carry it into our bloodstream, which carries it to the cells, tissues and organs that allow us to walk, talk and move.

Our lungs also remove carbon dioxide from our blood, through the air we breathe.

Once set up, solar energy is inexpensive to convert to a useful form, for every day use.
True/False

Answers

Answer:

Hey there!

The answer is true, making solar energy one of the most cost effective sources of energy.

Let me know if you need more help :)

Answer: True!

Explanation:

Once set up, solar energy is inexpensive to convert to a useful form, for every day use.

All you need to do is get the converter and convert the suns energy!

Rainforests around the world are being cleared at an alarming rate to make room for new communities, agriculture, mining, and roads, and to provide lumber for construction and furniture. How might the destruction of rainforests affect the balance of gases in Earth's atmosphere?

by increasing carbon dioxide and decreasing oxygen

by increasing carbon dioxide and increasing oxygen

by decreasing carbon dioxide and increasing oxygen

by decreasing carbon dioxide and decreasing oxygen

Answers

Answer:

(1) by increasing carbon dioxide and decreases oxygen

The destruction of the rainforests will affect the balance of gases in Earth's atmosphere by increasing carbon dioxide and decreasing oxygen.

Rainforests, like the Amazon, are the world's greatest concentration of living trees. Through the process of photosynthesis, these trees contribute to the composition of the atmosphere that we enjoy every day and depend on to survive.

All trees perform photosynthesis, the fuel for this is carbon dioxide. This process then releases oxygen into the atmosphere as a by-product. For this reason, the destruction of the rainforests will affect the balance of gases in Earth's atmosphere by increasing carbon dioxide and decreasing oxygen.

To learn more:

https://brainly.com/question/1478703?referrer=searchResults

Prokaryotes, like most living organisms, need movement to locate food and to survive.

Answers

Answer:

Explanation:

Prokaryotes, like most living organisms, need movement to locate food and to survive. If a prokaryote needed to move to a new food source, which structure would it use for locomotion?

DDT was once considered a "silver bullet" that would permanently eradicate insect pests. Instead, DDT is largely useless against many insects because they are resistant. Propose a hypothesis as to why these organisms have become resistant to the chemical.

Answers

Answer:

None of the individual insects should have possessed genomes that made them resistant to DDT.

Explanation:

Pesticides such as DDT have been considered one of the best way to kill insects in the past. But with time, farmers have observed that this is not an authentic method. This is because the insects can gain resistance to the pests if they are exposed to the pesticide again and again. Even they could transmit these resistant genes in their offsprings. Hence, DDT will become useless against insects over time.  

which question would you ask the client to obtain some environmental history of the pet? a. what medications does your pet take b. how many pets are in the house hold? c. has your pet had any previous surgeries? d. is your pet up to date with vaccinations?

Answers

Answer: B

Explanation:

That question lets you know how the pet is living on a daily basis. The other answer choices ask about medical history, but B asks about environment.

Help Res
Is there a symbiotic
relationship between
plants and water? If so,
what effect does it have on
their ecosystem?
A. Yes, there is a symbiotic
relationship that is beneficial to both.
B. No, this would not be a symbiotic
relationship
C. Yes, there is a symbiotic
relationship that is beneficial for the
plant but harmful to the water.

Answers

Answer: i think its a

Explanation:

Which sequence correctly represents the order of events during egg formation?

Answers

Answer:

The egg is made by the hen. It creates an embryo (the future chick, the little white spot that you see on the egg yolk) which will be fed by the yolk of the egg.

The yolk is a food reserve (called the yolk) for the chick in formation: by eating the yolk, the embryo becomes a chick.

The white consists of gelatinous proteins, which the future chick will eat during incubation, warm for 21 days under the hen.

The shell protects the embryo, the yolk and the white. It is made of two membranes that are made from calcium that the hen stores in her bones and intestines. In the chicken's belly, muscles spin the egg during training and give it its perfect shape.

The shell is made in such a way that it is difficult to the pan from the outside, but very easy to break it from the inside. So the hen does not crush her eggs when she is brooding, but the chick can come out easily!

It works pretty much the same for all birds, reptiles, fish ... that lay eggs. On them, moreover, oviparous animals.

Explanation:

Question 8 (5 points)
Which of the following best describes the mechanism by anti depressant
medications function?
Provide a positive rush of the neurotransmitter dopamin-
Made of artificial neurotransmitters that generate exciter
O Allow the body to prochyce more neurotransmitters such a
serotonin

Answers

Answer:

Help maintain a balance in neurotransmitters such as serotonin

Explanation:

Anti-depressant medications help the body maintain a balanced, homeostasis level of neurotransmitters, often serotonin.

Ms. Fisher is a 68-year-old woman with the classic symptoms of gallbladder disease. She is diagnosed with gallstones and is scheduled for surgery in 2 weeks . She asks you about the cause of gallstones and why she would develop them. How would you respond to her?

Answers

Answer: I will respond as this;

Gall stones develop from the chemical makeup imbalance of the bile inside the gall bladder which can increase in the levels of cholesterol, and cholesterol levels is increase, the excess form into stones.

Mrs Fisher can develop gall stones because she is 40 years and above and she is a female. Age and sex are the risk factors of gall stones

Explanation:

Gallstones are solid particles that are developed from bike cholesterol or bilrubin build up.

It Is caused by can increase in the levels of cholesterol, and cholesterol levels is increase, the excess form into stones.

Symptoms include .

The most common symptom is pain in the right upper part of the abdomen.

nausea and vomiting,

fever,

indigestion, belching, bloating,

intolerance for fatty or greasy foods, and

jaundice (yellowing

Mrs Fisher can develop gall stones because she is 40 years and above and she is a female. Age and sex are the risk factors of gall stones.

Women that had have children also are at risk of having gall stones.

The most effective treatment is surgical removal of the gallbladder, or cholecystectomy

Part D
Cervical pregnancies are extremely rare. Women undergoing cervical pregnancies often experience unusual vaginal bleeding. In extreme cases, they may require a hysterectomy (removal of the uterus). Why do you think the symptoms and risk occurs with this type of ectopic pregnancy?

Answers

Answer

The places where the fertilized egg got implanted are not appropriate

for the fetus´ development. These places lack space and needed tissues.  

Explanation:

Ectopic pregnancies occur most often in the Fallopian tubes,  which are the structures that transport the eggs from the ovaries to the uterus. But some other times, ectopic pregnancies might also occur in ovaries, abdominal cavity, or in the cervix.

These pregnancies are not viable, this means that the fertilized egg can not survive. However, the firsts stages of its development seem to be normal and involve an increase in tissues surrounding the egg.

As the places where the egg got implanted are not prepared for its development, they might be harmed. These areas do not have enough space,  nor nutritional tissues needed by the embryo. And as the fetus grows, it creates a constant pressure in the organ, that can make it burst. This provokes important bleeding that might cause the patient´s death if they are not properly assisted.    

At first, the woman might feel the normal symptoms of pregnancy, like the lack of the period during the firsts months or breast sensitivity.  But as the egg grows in the inappropriate place, there appear some new signs and symptoms, such as a light bleeding or pelvic pain. If the organ bursts, the bleeding might be so intense that the woman might be in danger.    

Answer:

Bleeding would occur because the baby is growing near the vaginal canal, which leads to the outside of the body. The cervix is the bottom portion of the uterus, so if the uterus is damaged, the entire uterus may need to be removed.

Explanation:

answer from Plato

for edmentum

One characteristic of fungi is that they have a cell wall made of chitin. What are two other characteristics of fungi? Produce spores and heterotrophic Contain membrane-bound nucleus and photosynthetic Heterotrophic and produce seeds Absorb food and photosynthetic

Answers

Answer:

Produce spores and are heterotrophic

Explanation:

All animals and fungi are heterotrophic and fungi produce spores which is how they reproduce.

Answer:

Produce spores and heterotrophic

Justin is a 24-month-old male with multiple food allergies, including milk, eggs, and peanuts. At six months old a cooking utensil that had been used to cook eggs touched his arm and his mother noticed a significant dermatological response. As a result, Justin’s pediatrician advised her to avoid eggs, cow’s milk, and peanuts in Justin’s diet until food allergy testing could be done at two years of age. Fortunately, Justin hasn’t had any negative reactions to soy milk. Food allergy testing has confirmed that Justin needs to continue to avoid cow’s milk and peanuts. His pediatrician plans to conduct food allergy testing annually and advises his mother that Justin will likely be able to outgrow the milk allergy. Justin is referred to a registered dietitian for an assessment of his growth and nutrition intake. His mother reports that he is able to feed himself and she has noticed no problems with chewing and swallowing. A 24-hour dietary recall shows Justin consumed the following foods:
Fortified soy milk (4 ounces), rice cereal, bananas, grilled soy-cheese sandwich, fortified soy milk (4 ounces), green beans, apricots, orange juice (8 ounces), apple slices, almond butter on toast, apple juice (8 ounces), roast turkey, baked potato, green peas, and fortified soy milk (4 ounces).
Date Weight Weight for age % Height Height for age % Weight for length %
12 months 23# a.____ 29" d.____ g.____
18 months 26# b.____ 31.5" e.____ h.____
24 months 29# c.____ 33.5" f.___ i.____
1) Complete the above table. Is Justin’s growth on track?
2) Does the diagnosis affect his nutritional needs? If so, how ?

Answers

Answer:

The correct answer is -

1. Based on height he is 50% and height he is 25%

2. The nutritional needs are normal due to the constant percentage in both height and weight throughout.

Explanation:

By the reference of the chart of growth rate presented by CDC,

These informations can be found-

Weight :

A= 50%

B=50%

C=50-75%

Height :

D=10-25%

E=25%

F=25%

Then the percentage of height by weight ratio

Height by weight%:

G=75-90%

H=75-90%

I=75-90%

Thus, the correct answer is -

1. Based on height he is 50% and height he is 25%

2. The nutritional needs are normal due to the constant percentage in both height and weight throughout.

Which one of the following statements about secreted proteins is incorrect? Secreted proteins: are likely to have received N-linked glycosylation in the rough ER. are synthesized on membrane-bound ribosomes, not free cytosolic ribosomes. that are constitutively expressed are exported as they are prepared, as opposed to being released as a response to a signal. cannot be exported into the extracellular space until the vesicle they are in fuses with the plasma membrane. travel directly from the rough endoplasmic reticulum into the trans face of the Golgi Apparatus.

Answers

Answer:

they travel directly from the rough endoplasmic reticulum into the trans face of the golgi apparatus

What type of feedback loop is breathing?

Answers

Any type of feedback could be breathing, and you wouldn’t notice

Since viruses are typically 20-200nm in diameter.. what is the best microscope to view them?

Answers

Answer:

electron microscope

Explanation:

Due to their extremely small size, the best microscope to view viruses would be the electron microscope.

The electron microscope has the capability to create resolved images of extremely small objects by utilizing electrons as a source of light instead of the visible light photon that is typical of light microscopes.

There are different two types of electron microscopes:

the scanning electron microscope, andthe transmission electron microscope.

A typical scanning electron microscope creates an image of an object by detecting knock-off electrons from the object, whereas a transmission electron microscope uses electrons that pass through the object to create the image of the object.

what causes metamorphoic rocks to form from existing rocks

Answers

Answer:

metamorphic rocks form from heat and pressure changing the original or parent rock into a completely new rock.

2. If a nucleus has 8 chromosomes, how many chromosomes will be present after Telophase I? 3. What advantage does crossing-over have in reproduction? 6. Compare and contrast spermatogenesis and oogenesis (where do they occur, how many gametes are formed, when do they develop, etc):

Answers

Answer:

Telophase I: 8 chromosomes composed of two sister chromatids  

Crossing over refers to the mechanism of recombination, this process being characterized in that increases the genetic variation

Spermatogenesis refers to the mechanism of formation of male gametes (sperms), while oogenesis is the process of formation of female gametes (oocytes)

Explanation:

Telophase I is the final step in meiosis I. During telophase I, the nuclear membrane is formed around the decondensed set of chromosomes and the nucleoli reappear.  

Spermatogenesis refers to the process by which haploid male germ cells (sperms) are generated within the seminiferous tubules in the testis, while oogenesis refers to the process of differentiation of the female egg cells (ova or oocytes) in the ovaries. Spermatogenesis is known to produce small and motile spermatic cells, while oogenesis is known to produce bigger non-motile oocytes.

Other Questions
The volume of a certain gas increases by 25%. Complete the following statement.The new pressure will beof the original pressure.120%75%80%125% What conclusion is best supported by the data in this table? (4 points)A. Workers today make more money thanks to the fair labor standards actB.Rising minimum wages have made the fair labor standards act irrelevantC. the fair labor standards act still affects workers wages todayD. minimum wages must rise at a steady rate according to the fair labor standards act. Tina had d dollars. She bought three cupcakes for her and her friends, which cost c dollars each. How much money does she have left after being so nice? Steve likes to entertain friends at parties with "wire tricks." Suppose he takes a piece of wire 56 inches long and cuts it into two pieces. Steve takes the first piece of wire and bends it into the shape of a perfect circle. He then proceeds to bend the second piece of wire into the shape of a perfect square. What should the lengths of the wires be so that the total area of the circle and square combined is as small as possible When a country produces on its production possibilities curve, then this country's unemployment is expected to be at one of its lowest rates, however, prices in this country are not expected to be relatively low.a. Trueb. False How did the Khmer Rouge attempt to remove Western influences from Cambodia? Select all that apply. A) They made people study and then practice Theravada Buddhism. B) They moved city people to the country to work in the fields. C) They exiled anyone who had studied or traveled to the West. D) They killed or starved to death all who disagreed with them. Determine the area under the standard normal curve that lies between (a) Upper Z equals negative 0.12Z=0.12 and Upper Z equals 0.12Z=0.12, (b) Upper Z equals negative 0.35Z=0.35 and Upper Z equals 0Z=0, and (c) Upper Z equals 0.02Z=0.02 and Upper Z equals 0.82Z=0.82. (a) The area that lies between Upper Z equals negative 0.12Z=0.12 and Upper Z equals 0.12Z=0.12 is nothing. (Round to four decimal places as needed.) (b) The area that lies between Upper Z equals negative 0.35Z=0.35 and Upper Z equals 0Z=0 is nothing. (Round to four decimal places as needed.) (c) The area that lies between Upper Z equals 0.02Z=0.02 and Upper Z equals 0.82Z=0.82 is nothing How does a reflection across the y-axis change the coordinates of a shape? What is the length of JM in the given figure? Just need to know the elements of (A n B) Find the number. PLEASE HELP!!! A user reports that a file they shared out on their computer for another network user is not accessible to the third party. The user insists they specifically gave this third party Allow rights for Read and Write access. Which of the following could explain the problem at hand?A. The parent folder has explicit Allow rights set for this user.B. The parent folder has explicit Deny rights set for this user.C. The user forgot to share the parent folder and only shared the specific file.D. The parent folder likely has the "archive" attribute enabled. x^2+8x=20 Solve by completing the square what is the term that describes what occurs when genetic drift results in the formation of a new population that has less variation than the original population had A. Speciation B.Founder effect C.Mutation D. Sexual selection . A large scuba tank with a volume of 18 L is rated for a pressure of 220 bar. The tank is filled at 20 C and contains enough air to supply 1860 L of air to a diver at a pressure of 2.37 atm (a depth of 45 feet). Was the tank filled to capacity at 20 C? An integer is 7 more than 2 times another. If the product of the two integers is 60, then find the integers. my father gave him many advices correct this sentence A box with a hinged lid is to be made out of a rectangular piece of cardboard that measures 3 centimeters by 5 centimeters. Six squares will be cut from the cardboard: one square will be cut from each of the corners, and one square will be cut from the middle of each of the -5 centimeter sides . The remaining cardboard will be folded to form the box and its lid . Letting x represent the side-lengths (in centimeters) of the squares, to find the value of that maximizes the volume enclosed by this box. Then give the maximum volume. Round your responses to two decimal places. PLEASSSSEEE HELP ! what number belongs in the box ? y=200+ ? x The core of an optical fiber has an index of refraction of 1.35 , while the index of refraction of the cladding surrounding the core is 1.21 . What is the critical angle c for total internal reflection at the corecladding interface?