Another step in PCR requires small single-stranded DNA primers to anneal to a target sequence on denatured DNA. Two primers are used. Ideally these primers will have similar melting temperatures. The melting temperature is defined as the temperature at which 50% of the DNA is in the single-stranded form. Which of the following primers will have the highest melting temperature?
A. GGGGAAATTTCCCC.
B. AAAAGGGCCCTTTT.
C. AAAAGGCCTTTT.
D. GGGAAATTTCCC.

Answers

Answer 1

Answer:

A. GGGGAAATTTCCCC.

Explanation:

When calculating the melting temperature in the DNA primers, we use the formula:

( 2 × ( A + T ) ) + ( 4 × ( G + C ) )

From the above formula; it is obvious that G and C have a greater tendency of participation when determining which DNA primers that have the highest melting temperature.

A.  GGGGAAATTTCCCC = 8

B. AAAAGGGCCCTTTT   = 6

C. AAAAGGCCTTTT        = 4

D. GGGAAATTTCCC        = 6

From the given options, we can see that option A have the highest component of G and C.


Related Questions

Why do stem cells have the potential to
cure many human diseases?

Answers

Beacuse you are born with them they help stop it

This process does not require the cell to use ______ to move molecules.
It is an example of _______ transport.

Answers

Answer:

Energy, passive

Explanation:

This process does not require the cell to use energy  to move molecules. It is an example of passive transport.

Plz help I’ll mark brainliest

Answers

the answer is A,photosynthesis

Answer:

Through Photosynthesis

What process is partially responsible for creating local climates in urban areas

Answers

Urban heat islands effect

What would you feel while singing "ang mga ibon ay mahal ng diyos"​

Answers

Answer:

Uhm I can't answer that for you just, because that really depends on how you feel about the song.

Explanation:

While monosaccharides typically all have a molecular formula that is a multiple of CH20, they differ in factors such as the length of the carbon skeleton or the orientation of their atoms around asymmetric carbon atoms. What is the impact of this variation in molecular structure? 1.) Different monosaccharides will form disaccharides with the same properties. 2.) Cells will use more energy to metabolize a wider variety of polysaccharides. 3.) Molecules that all share the same bonding pattern but have different properties will be produced. 4.) Sugars with different properties that can build a wide variety of carbohydrates will result.

Answers

Answer:

Sugars with different properties that can build a wide variety of carbohydrates will result.

Explanation:

This is the correct answer.This is because the arrangement of the monosaccharides units though the linking of the monomers with the glycosidic bonds via condensation reaction  goes a long way to determine the orientation of the monosccharides and therefore the chemical properties  displays.

Therefore when monosaccharides are linked by glycosidic bonds  in different ways to form diasaccharidesa and polysacharrides,these macromolecueles  display different chemical properties and physical properties  from one another.

This is evident in cellulose((beta-glucose) whose 3-helix structure    strengthened the wall of green plants.This orientation ensures that the hydrogen of -OH group is attached to the Oxygen of the same  OH in the same molecule,or to that of another molecule.This formed hydrogen bond strong enough to form microfibrils which are held by fibres.

This is different from the structure of glycogen(from alpha 1,4 and 1,6-glycosidic bonds) despite the two been formed from monoscacharides. This is also evident in the structure of  starch  formed by alpha glucose

It is the orientation of the monomoers of the monosccharides in space that determines this different properties

A scientist was investigating why several fish caught from a local stream displayed similar mutations. He found that the water temperature of the stream was elevated since an industrial plant began discharging heated water into the stream. The scientist concluded that increased water temperature during the egg phase led to the mutation in the fish. According to this conclusion, the mutation was caused by

Answers

Answer: Increased water temperature caused by the industrial plant.

Explanation: this is explained in the text lol.

Answer:

An Environmental Condition

Explanation:

It explains in the text that it started when an electrical plant began discharging heated water into the stream.

Drag each label to the correct location on the image.
A diagram of an animal cell is shown below. Each arrow points to a different organelle. Correctly label each organelle.

centriole
cell
membrane
ribosome
Golgi
apparatus
nucleus
rough
endoplasmic
reticulum
mitochondrion
smooth
endoplasmic
reticulum

Answers

hope this helps :D (I know a few are missing sry <3)

Name a protein structure

Answers

Answer:  Types; Examples : Transport (Hemoglobin, albumin )

Structural, (Actin, tubulin, keratin ), and Hormones, (Insulin and thyroxine).

Explanation: Protein structure is the three-dimensional arrangement of atoms in an amino acid-chain molecule. Proteins are polymers – specifically polypeptides – formed from sequences of amino acids, the monomers of the polymer. A single amino acid monomer may also be called a residue indicating a repeating unit of a polymer.

There are seven types of proteins: antibodies, contractile proteins, enzymes, hormonal proteins, structural proteins, storage proteins, and transport proteins.

It is convenient to describe protein structure in terms of 4 different aspects of covalent structure and folding patterns. The different levels of protein structure are known as primary, secondary, tertiary, and quaternary structure. The primary structure is the sequence of amino acids that make up a polypeptide chain.

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Have a good day!     :)

you don't have to eat a variety of foods to get the nutrients your body needs. True or false.

Answers

This is trueeeeeeee yeah

Answer:

True

Explanation:

Question: What do you think has to happen for
the bond between the nucleotides to be broken:
a) More water molecules must be removed.
b) A water molecule must be added.

Answers

Answer:

A water molecule must be added

Which of the following performs the phosphorus cycle A) Autotrophs C) Carnivore B)Heterotrophs D) Herbivore

Answers

Answer:

The autotrophs are the ones that perform the phosphorus cycle.

Explanation:

A is correct because the autotrophs are the organisms that are able to consume the inorganic phosphates, use them, and release parts of them in the other spheres, thus contributing to the phosphorus cycle.

B is not correct because the heterotrophs are not organisms that can process inorganic matter and produce their own food, instead, they are dependent on the autotrophs to do that.

C is not correct because the carnivores are not able to consume and process inorganic phosphates, with their food sources being based around the primary and secondary consumers.

D is not correct because the herbivores use the producers (autotrophs) as their food source, and they are not able to process inorganic phosphates on their own.

What are three things that eukaryotic cells have? Write in two sentences

Answers

Answer:

They have membrane bound organelles as well as a membrane bound nucleus.

Here's extra: "Endoplasmic reticulum, microtubules, and the Golgi apparatus are unique to eukaryotic cells, and will not be found in prokaryotes."

HELP ME PLEASE IM STRUGGLING
How does rainfall positively impact the species and evenness of the ecosystem?

Answers

Answer: it gives  water to the plants and water for animals to drink

PLEASE HELP. I WILL GIVE YOU BRAINLIEST!

Which terms correctly identify the indicated structures in this sketch of a cell viewed under a microscope?

Drag and drop a term to match each label.

Answers

Answer:

A: The right answer is Vacuole

Vacuoles are organelles present in plant cells.  It is a large single structure, of variable shape according to the cells, delimited by a lipid membrane called tonoplast.

The vacuoles allow the storage of water, ions, sugars, ... and by magnifying, play an important role in the growth of the plant.

B: The right answer is Mitochondria

Mitochondria are organelles present in eukaryoptic cells. The mitochondrion is composed of a double membrane, the inner membrane being folded into mitochondrial ridges.

Mitochondria are the organelles that produce the energy of the cell. The mitochondria thus have a role of power station for the cell. They are the ones who perform cellular respiration.

C: The right answer is Cell membrane (the inside layer)

All cells (prokaryotic and eukaryotic) are separated from their environment by a palsmic membrane.

The plasma membrane is the line of demarcation between the cell and its environment (= envelope of the cell).

It determines what goes into the cell and what comes out of it. Like all biological membranes, the plasma membrane has a selective permeability, that is to say it is allowed to pass through certain substances more easily than others.

The plasma membrane consists of a double lipid layer in which proteins are inserted.

D: The right answer is Chloroplast

Chloroplasts are important organelles specific to plant cells. Chloroplasts appear green ... They contain chlorophyll, a pigment that captures solar energy.

Choroplasts perform photosynthesis, that is, they absorb sunlight and use it for the manufacture (synthesis) of organic compounds from carbon dioxide and water.

E: The right answer is Lysosome

The lysosome is a small spherical structure (a vesicle) delimited by a lipid membrane located in the cytoplasm of eukaryotic cells. The membrane contains ion channels (proton pumps) that allow the active entry of H + ions, in order to maintain an acid pH (between 3.5 and 5) within the lysosomal vesicle.

Lysosomes are organelles of eukaryotic cells that contain a mixture of digestive enzymes used to degrade macromolecules.

Lysosomes are considered the "stomach" of the cell.

F: The right answer is Endoplasmic reticulum

The endoplasmic reticulum are organelles of eukaryotes that extend throughout the cytoplasm (like a labyrinth). These are organelles that produce proteins and play a vital role in the development of the other membranes of the cell.

Explanation:

Dont forget my brainliest

The parts of a flower

Answers

Answer:

A typical flower has four main parts or whorls known as the calyx, corolla ,androecium and gynoecium

Explanation:

the outermost whirl has green leafy structures know as the sepals. The sepals collectively called the calyx help to protect the unopened bud.

Answer:

The parts of a flower is in the above picture.

How are proteins and carbohydrates similar?

Answers

Answer:

Proteins consist of units called amino acids, strung together in complex formations. Because proteins are complex molecules, the body takes longer to break them down. As a result, they are a much slower and longer-lasting source of energy than carbohydrates. There are 20 amino acids.

Answer:

they both give us energy

Explanation:

Consider a population of Clostridium difficile living in the human gut. Regular ingestion of an antibiotic is a selective process on the gut ecosystem. Which of the following events could be considered adaptive mutations for C. difficile in this environment? A. Acquisition of a plasmid with a gene encoding an enzyme to inactivate the antibiotic B. Deletion of the gene encoding a restriction endonuclease C. Duplication of the gene encoding flagellin D. Point mutation changing a single amino acid in a membrane transporter

Answers

Answer:Acquisition of a plasmid with a gene encoding an enzyme to inactivate the antibiotic

Explanation:

Clostridium difficile is a Gram-positive gastroimtestinal bacteria that forms spore it cause a range of diseases like antibiotic-associated diarrhea (AAD).

C. difficile genome harbors a variety of resistance genes that makes it resistance to different classes of antibiotics. Analysis conducted on C. difficile 630 genome identified genes encoding β-lactamase-like proteins and penicillin-binding proteins (PBPs) as one that mediate the resistance to the β-lactam antibiotics such as penicillin and cephalosporins this is done by an enzymes that unlocks the drug active site making the bacteria resistance to it.

In which specimen were cells first identified?

Answers

Answer:

The specimen where cells were first identified was cork.

hope this helps have a good day

Helpppp!!

how does pinocytosis work

Answers

Answer:

In cellular biology, pinocytosis is a type of endocytosis that involves the taking in of extracellular fluid into the cell. By utilizing this process, the cell can draw fluids and even solutes (as long as they are dissolved in fluids) into the cell with only a small amount of energy (ATP) needed.

Explanation:

vote brainlyest

Lipids such as fats serve as a stored form of energy and are required for varius functions of the body

Answers

Answer:

uhm this is true is that is what your asking?

Explanation:

Lipids such as fats serve as a stored form of energy and are required for various functions of the body. The body needs to digest and absorb the fats we consume so that it can accomplish these functions.

what is biomimicry pls someone help me!!! i need it asap and i’m so confused

Answers

Bio mimicry is like copying models, systems, and elements of nature for the purpose of solving human problems

In 1918, the tsar (king) and tsarina (queen) of Russia and their five children were presumably assassinated and buried in an unmarked grave. Later, several women came forward and claimed that they were in fact Anastasia, one of the daughters whose bones had not been found. More recently, forensic scientists have extracted DNA, including mtDNA, from the skeletal remains of the tsar (father), tsarina (mother), and children. Which of the following samples of mtDNA would NOT be helpful in confirming that a woman is Anastasia?
a. the tsar and tsarina and living relatives of each of them.
b. each of the claimants or their living children.
c. the tsar, tsarina, and the other assassinated children.
d. the tsar and tsarina only.
e. the tsarina and the claimants.

Answers

The correct answer is B. Each of the claimants or their living children.

Explanation:

One of the basic principles in genetics establishes genes are passed from the parents to their offspring. Indeed, children receive 50% of genes from each parent. This implies the daughter of the tsar and tsarina will have share part of the DNA including mtDNA (mitochondrial DNA) of her mother, father, and siblings.

According to this, to prove if someone is Anastasia or not, it is possible to use the mtDNA sample from the tsar, tsarina, or death children but not from the claimants. This is because the claimants are not confirmed relatives of the tsar or the tsarina, and therefore their DNA cannot be used to determine if a woman is the daughter of the tsar and tsarina. Indeed, a woman claiming to be Anastasia can share DNA with another claimant and this does not prove they are related to the tsar and tsarina or that the woman is the real Anastasia.

What is the name of the solid part of Earth, including rock, minerals, and metals?

Answers

Answer:

Lithosphere is the name of the solid part of Earth, including rock, minerals, and metals.

Answer:

Geosphere/Lithosphere

Explanation:

The answer would be lithosphere but for this question it wouldn't' be because I took the test.So another word for lithosphere would be geosphere.These answers are both correct just that sometimes the test would only show one of these.

Explain how available energy changes at each trophic level. What happens to the energy that is lost?

Answers

Answer:

Energy decreases as it moves up trophic levels because energy is lost as metabolic heat when the organisms from one trophic level are consumed by organisms from the next level. Trophic level transfer efficiency (TLTE) measures the amount of energy that is transferred between trophic levels

need help please ):

Answers

It’s C ) Mitochondria-makes energy
I’m like 70% sure it’s ribosomes

A specific ligand is polar in nature. Where is the ligand-binding portion of the receptor most likely to be located?

Answers

Answer:

on the extracellular side of the cell membrane

Explanation:

Ligands can be defined as signaling molecules that interact with receptors localized on target cells. Protein ligands are generally polar and soluble in water. In consequence, they tend to bind with extracellular domain receptors localized on the cell surface, on the outside surface of the cell. Some examples of protein ligands include, among others, hormones (e.g., insulin), growth factors, neurotransmitters, etc.

Breathing occurs when the changes in air pressure causes air to flow into or out of your lungs as you inhale or exhale.

true or false

Answers

Answer:

true

Explanation:

is oxygen an atom or molecule​

Answers

Answer:

i think its a molecule

Explanation:

Oxygen is found naturally as a molecule.

Which of the following are functions (jobs) of the cell membrane?

Answers

Answer:

Biological membranes have three primary functions: (1) they keep toxic substances out of the cell; (2) they contain receptors and channels that allow specific molecules, such as ions, nutrients, wastes, and metabolic products, that mediate cellular and extracellular activities to pass between organelles and between the cell and the outside environment; and (3) they separate vital but incompatible metabolic processes conducted within organelles.

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The council members of a small town have decided that the earth levee that protects the town from flooding should be rebuilt and strengthened. The town engineer estimates that the cost of the work at the end of the first year will be $68,000. He estimates that in subsequent years the annual repair costs will decline by $7000, making the second-year cost $61,000; the third-year $54,000, and so forth. The council members want to know what the equivalent present cost is for the first 5 years of repair work if interest is 6%. he length of a rectangle is 3.7 inches when rounded to the nearest tenth of an inch. Select all of the following that could be the actual length of the rectangle. Mr. and Mrs. Wagner are getting ready to drive across the USA from Philadelphia, PA to Los Angeles, CA in five days. The distance is approximately 3,000 miles. The average price of gasoline is about $2.10 per gallon. They plan to stay in a hotel for four nights and budget the cost of meals be about $45 per day per person. Can someone please tell me what the equation of the line is in standard form by using the graph? I need to sleep and this is due tomorrow. Whoever answers, Ill mark you as brainliest!! I need help writing a paragraph talking about culture but relating to "art". PLEASE DON'T GET IT OFF THE WEB Summarize the steps that occur in the process of chromosomal DNA replication. Please place the following steps in the proper order. A. DnaA proteins bind to the origin of replication, resulting in the separation of the AT-rich region.B. DNA helicase breaks the hydrogen bonds between the DNA strands, topoisomerases alleviate positive supercoiling, and single-strand binding proteins hold the parental strands apart.C. Topoisomerases unravel the intertwined chromosomes, if necessary.D. Primase synthesizes one RNA primer in the leading strand and many RNA primers in the lagging strand, and DNA polymerase III synthesizes the daughter strands of DNA. E. DNA polymerase I removes the RNA primers and fills in that region with DNA, and DNA ligase covalently links Okazaki fragments together. F. Replication continues until the two replication forks meet on the other side of the circular bacterial chromosome. Two new wind-farm tower projects are proposed for a small company that installs them in south western Pennsylvania. Project A will cost $250,000 to complete and is expected to have an annual net cash flow of $75,000. Project B will cost $150,000 to complete and should generate annual net cash flows of $52,000. As a small company, the owner and senior management team are very concerned about their cash flow. Use the payback period method and determine which project is better from a cash flow standpoint. Show your work and include any formulas used to calculate PP. Can someone please help me How does the side length of the square relate to the square root? Tariffs and subsidies are both types ofO restrictionsO economic benefits.economic penalties.o incentives PLEASE HELP ME What does the phrase the menace of the year mean? A) the possibility of life after death B) the threat of passing C) the years that cause anger D) the years that fuse frustration what happened to George Washington and his small Force in May 1754 A box is sliding along a frictionless surface and gets to a ramp. Disregarding friction, how fast should the box be going on the ground in order to slide up the ramp to a height of 2.5 meters, where it stops? (Use g = 9.8 m/s2.) What is Las Casas overall opinion of the people of the Indies? How did the caste systemgive power? True or false: Informative writing pieces must have a formal style of writing. True Or False She paid with a $100 bill and received $32.16 in change. If sales tax is charged at a rate of 6%, what was the price for one candle holder before taxes? 15. A student graphed f(x) = x and g(x) = f(x) + 3 on the same coordinate grid. Whichstatement describes how the graphs of fand g are related? A. The graph of f is shifted 3 units up to create the graph of g.B. The graph of f is steeper than the graph of g.C. The graph of f is shifted 3 units down to create the graph of g.D. The graph of f is less steep than the graph of g. Pat has 9cards. Frank has 2more cards than Steve. Steve has 3cards. How many more cards does pat have than frank ? yeah I dont get this at all whatsoever.