After conducting SDS-PAGE, you will conduct a staining/destaining procedure, which allows for visual identification of the proteins in your gel. This procedure requires the following staining solution: Coomassie Blue staining solution: 0.1% Coomassie Blue dye (dry powder) in 50% methanol, 10% acetic acid.

Required:
How to make 1 L of this solution?

Answers

Answer 1

Answer:

Add 700mL of water.

Explanation:

The technique of separation known as the Sodium Dodecyl Sulfate Poly-Acrylamide Gel Electrophoresis in which its acronym is SDS- PAGE is a very important aspect of Chemistry and Biochemistry as the technique make use of molar mass when it work in seperating proteins.

From the question we have the following parameters or information:

Case 1=> 50% of methanol which is the same as saying 50mL in 100mL of water.

Case 2=> 0.1% Comasse Blue dye (dry powder) which is the same as saying 0.1g in 100mL of water.

Case 3=> 10% acetic acid is the same as saying 10mL in 100mL of water.

Adding the three together, we have ;

50mL in 100mL of water + 0.1g in 100mL of water + 10mL in 100mL of water.

The volume of water in the three = 100 mL + 100mL + 100mL = 300mL.

Therefore, there is need to add 700mL of water to make 1L.

That's 700mL of water + 300mL = 1000mL = 1L


Related Questions

This is for science! I have until tomorrow to finish this and I need your help. Can you guys answer one of the three matters? Pleasel

Answers

I've attached a picture down below for you, if this will help. If you need further explanation, please ask me :)

1. Determine the mass of 2.5 mol of AgNO3

Answers

Explanation:

1 gram of AGNO3 is 169,87 g/mol

2.5 mole = 2.5×16987

= 42467

A student says that since the atomic theory is just a theory, it should not be considered useful. Which statement best argues against the student’s opinion?

Scientific theories change over time.
Scientific theories are the results of many experiments and observations.
Scientists often do not agree about specific details of scientific theories.
Scientists often propose competing theories.
Scientific theories do not become Scientific Laws.

Answers

Answer:

srry

Explanation:

Answer:

Scientific theories are the results of many experiments and observations.

Explanation:

Sooo BBBBBBBBB

What happens as the activation energy increases?
The pressure of the system decreases.
The kinetic energy of colliding molecules changes.
The reactant surface area decreases.
The reaction begins to slow down.

Answers

Answer:

the kinetic energy of colliding molecules changes

Average kinetic energy is the average sum of all kinetic energy of all the molecules of gases. Therefore, the correct option is option B that is the kinetic energy of colliding molecules changes.

What is kinetic energy?

It is a energy which is possess by a moving object. It is very difficult to find the correct value of kinetic energy so we find average kinetic energy. Which is very easy to calculate as compared to calculating kinetic energy. It is not dependent on the type of gases or mass of gases.

Mathematically,

K.E=1/2mv²

Where,

m= mass of object

v= velocity of object

As the activation energy increases, the kinetic energy of colliding molecules changes.

Therefore, the correct option is option B that is the kinetic energy of colliding molecules changes.

Learn more about the kinetic energy, here:

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how many moles of each atom in the formula HClO3

Answers

Answer:

H has one mole

Cl has one mole

O has three mole

Explanation:

There is a single Hydrogen, thus there is one mole, this also applies to Cl.

With Oxygen, there are three O's, thus there are three moles of Oxygen.

There are 1 mole of H, 1 mole of Cl, and 3 moles of O.

Number of moles in molecules

The number of moles of an atom present in a molecule is classified as the number located in the lower right corner of that atom in the molecule, for example, in the water molecule below:

                                                 [tex]H_2O[/tex]

you have 2 moles of hydrogen and 1 mole of oxygen.

Thus, in the HClO3 molecule, there are 1 mol of hydrogen, 1 mol of chlorine and 3 mol of oxygen.

Learn more about mole calculation in: brainly.com/question/2845237

3.0m3 of sulfer dioxide is reacted with 2.0dm3 of oxygen . What volume of sulfur trioxide is form?

Answers

Answer:

3.0 dm^3 of sulphur trioxide is formed

Explanation:

From Gay Lussac's law of combining volumes;

The equation is;

                                                  SO2(g) + 1/2O2(g) ------> SO3(g)

Reacting volumes                       1 vol          1/2 vol             1 vol

Initial volumes                           3.0 dm^3    2.0 dm^3       0.0 dm^3

Final volumes                            0.00 dm^3  1.5 dm^3        3.0 dm^3

Hence, 3.0 dm^3 of sulphur trioxide is formed.

A. The ivy had more genes than the cactus

B. Natural selection choose to kill the cactus

C. The ivy had good genes, but the cactus had bad ones

D. The environmental conditions were right for the ivy but wrong for the cactus

Answers

the answer is c ..............::

4 points 15. The diagram below shows the relative positions of Earth, the moon, and the sun. The moon is shown at four possible positions around Earth. 3 Sunlight Sun Earth mo Which position of the moon could result in a lunar eclipse? В. 2 A B ​

Answers

Answer:

b c

Explanation:

Answer: b c

Explanation: i did test

Analyze Between visits 1 and 7, what kind of ecological succession is shown? Explain your answer.​

Answers

Answer:

Is there supposed to be a picture??

Explanation:

Determine the energy released per kilogram of fuel used.

Answers

Answer:

what is the energy rate? or how much energy do you have.

Explanation:

what is the answer?!!?
Plzzzz help

Answers

Answer:

The planet would stop

Good luck!

The planet will stop I agree

Why is classification important?

Answers

it is important because it helps scientists to solve problems and do more research
If we were to classify organisms into groups based on their ancestry, characteristics, evolutionary traits, etc., we would have a much easier time studying them in detail. It's like sorting out your school assignments. You might want similar subjects grouped together, and so you can find everything much faster.

stoichiometry is hawd

Using the following reaction:

C12H22O11 + 12 O2 → 12CO2 + 11H2O + energy

How many grams of carbon dioxide, CO2, are produced from metabolizing 448g sucrose?

Answers

Answer:

691 g CO₂

General Formulas and Concepts:

Math

Pre-Algebra

Order of Operations: BPEMDAS

Brackets Parenthesis Exponents Multiplication Division Addition Subtraction Left to Right

Chemistry

Atomic Structure

Reading a Periodic TableMoles

Organic

Organic Compounds CₓHₙ

Stoichiometry

Using Dimensional AnalysisAnalyzing Reactions RxNExplanation:

Step 1: Define

[RxN - Balanced] C₁₂H₂₂O₁₁ + 12O₂ → 12CO₂ + 11H₂O + Energy

[Given] 448 g C₁₂H₂₂O₁₁ (sucrose)

[Solve] grams CO₂ (carbon dioxide)

Step 2: Identify Conversions

[RxN] 1 mol C₁₂H₂₂O₁₁ → 12 mol CO₂

[PT] Molar Mass of C - 12.01 g/mol

[PT] Molar Mass of H - 1.01 g/mol

[PT] Molar Mass of O - 16.00 g/mol

Molar Mass of C₁₂H₂₂O₁₁ - 12(12.01) + 22(1.01) + 11(16.00) = 342.34 g/mol

Molar Mass of CO₂ - 12.01 + 2(16.00) = 44.01 g/mol

Step 3: Stoich

[DA] Set up conversion:                                                                                   [tex]\displaystyle 448 \ g \ C_{12}H_{22}O_{11}(\frac{1 \ mol \ C_{12}H_{22}O_{11}}{342.34 \ g \ C_{12}H_{22}O_{11}})(\frac{12 \ mol \ CO_2}{1 \ mol \ C_{12}H_{22}O_{11}})(\frac{44.01 \ g \ CO_2}{1 \ mol \ CO_2})[/tex][DA] Multiply/Divide [Cancel out units]:                                                           [tex]\displaystyle 691.119 \ g \ CO_2[/tex]

Step 4: Check

Follow sig fig rules and round. We are given 3 sig figs.

691.119 g CO₂ ≈ 691 g CO₂

how do plants release carbon into the atmosphere

Answers

Answer:

Plants release more carbon dioxide into atmosphere than expected. ... Plants use photosynthesis to capture carbon dioxide and then release half of it into the atmosphere through respiration. Plants also release oxygen into the atmosphere through photosynthesishesis to capture carbon dioxide and then release half of it into the atmosphere through respiration. Plants also release oxygen into the atmosphere through photosynthesis.

I don’t think plants release carbon dioxide into the atmosphere, do you mean oxygen? Because if you did, plants do photosynthesis and release oxygen in the environment.

This is for science! I really need help.

Answers

Answer

Solid

liquid

Gas

Explanation:


the three states of matter are solid, liquid, and gas

S plus 11r minus fifteen​

Answers

Answer:

s+11r-15

Explanation:

HELP! WILL GIVE BRAINLIEST DUE SOON

Dome Mountains are formed when:

A: plateaus are eroded away

B: faults rub against each other

C: magma escapes from the crust

D: magma pushes up sedimentary rock

Answers

Answer:

I am not the best to answer this but I think it would be B

Explanation:

Dome Mountains are formed from hot molten material (magma) rising from the Earth's mantle into the crust that pushes overlying sedimentary rock layers upward to form a “dome” shape.
So it’s D.

The electron configuration for Zn2 is [Ar] 3d10 and NOT [Ar] 4s23d8. What experimental evidence has been collected that would allow one to conclude that the [Ar]3d10 is correct

Answers

Answer:

Zn2+ is colourless

Explanation:

We know that transition metal salts are usually coloured due to the possibility of d-d transition.

This d-d transition can only occur when there are vacant d-orbitals. The electronic configuration [Ar] 4s23d8 suggests the presence of vacant d-orbitals and the possibility of the compounds of Zn2+ being coloured.

However, the absence of colours in Zn2+ compounds shows that there is no d-d transition(electronic) spectra observed for Zn2+ because the d orbitals are completely filled. This means that the correct electronic configuration of the ion is [Ar] 3d10.

what happens to ammonia when reacts with heated sodium?​

Answers

When dry ammonia is passed over heated sodium in absence of air, the product formed is sodium amide. - When dry ammonia is passed over heated sodium in absence of air, sodium amide is produced. - Sodium amide aka sodamide is mainly used as a strong base in organic chemistry.

Answer:

When dry ammonia is passed over heated sodium in absence of air, the product formed is sodium amide. - When dry ammonia is passed over heated sodium in absence of air, sodium amide is produced. - Sodium amide aka sodamide is mainly used as a strong base in organic chemistry.

Explanation:

Classify each of the substances as an atomic element, molecular element, molecular compound, or ionic compound. Provide one sentence explanation for each. a. fluorine b. N2 O c. silver d. K2 O e. Fe2 O3 g

Answers

Answer and Explanation:

a. fluorine ⇒ atomic element

Fluorine (F) is a chemical element because it is a pure substance that cannot be decomposed into simpler substances.

b. N₂O ⇒ molecular compound

We can see that N₂O (nitrous oxide) is a molecule composed by two different atomic elements: nitrogen (N) and oxygen (O). Thus, it is a molecular compound.

c. silver ⇒ atomic element

Silver is a chemical element with the symbol Ag. It is a pure substance which cannot be decomposed into simpler substances.

d. K₂O ⇒ ionic compound

Potassium oxide (K₂O) is composed by a metallic element (potassium, K) and a non-metallic element (O). Thus, there is a difference in the electronegativity of the chemical elements, so the substance can dissociate into ions. In consequence, it is an ionic compound.

e. Fe₂O₃ ⇒ molecular compound (with ionic character)

Iron(III) oxide (Fe₂O₃) is composed by iron element (Fe), which is a metal, and oxygen element (O), which is a non-metal. Since it is a Metal- Non-Metal combination, it would be an ionic compound. The difference in electronegativity between Fe and O is not high (<2.0) in comparison with other ionic compounds, so Fe₂O₃ is considered as a polar covalent compound (it is between an ionic compound and a molecular compound).

Fall is usually a little warmer than spring. And yet, the Sun's path is the same in both seasons. Select all that apply.PLEASE ANSWER CORRECTLY I WILL GIVE BRAINLIEST!-THIS IS SCIENCE

Answers

I don't know the answer sorry I would answer if if I did

Which statement does NOT correctly compare boron with another element?
Boron is a better conductor of electricity than sulfur.
Boron conducts electricity as well as copper does.
Boron is a ſolid at room temperature, but argon is a gas.
O Boron is less malleable than silver.

Answers

Statement #2 does not correctly compare boron to copper. B Boron is a semi metal so it can conduct electricity however metals (like Cu copper) are much better conductors of electricity.

What is the mass in grams of 5.2 moles of NaCl?
303.89 g
0.09 g
119.6 g
1828

Answers

Explanation:

mole=mass/molar mass

mass= mole×molar mass

m= 5.2×( 23+35.5)

m= 5.2×58.5

m= 304.2g

What is the chemical formula for a molecule of salt?

Answers

Answer:

Su fórmula molecular es Na Cl y es el producto de la base hidróxido sódico (NaOH) y ácido clorhídrico, H Cl. En general, las sales son compuestos iónicos que forman cristales. Son generalmente solubles en agua, donde se separan los dos iones. Las sales típicas tienen un punto de fusión alto, baja dureza, y baja compresibilidad.

Explanation:

Use Hess’s Law and solve for the enthalpy change of the following rxn:
12 P(s) + 10 Fe2O3 (s) -> 3 P4O10 (s) + 20 Fe (s)


2 C2H6 (l) + 7 O2 (g) -> 4 CO2 (g) + 6H2O (g)

Answers

Dang that is messed up you have a EVIL teacher to do that to you

What is the percent yield if the theoretical yield of ChCl3 is 14.50 g but the actual yield is 12.33 g?

Answers

Percentage is a ration of part to a whole. In our case, in perfect conditions, yield is theoretical and thusly considered larger, a whole. While actual yield is less because of imperfect conditions. The percentage is,

[tex](12.33\cdot 100)/14.5\approx85.03\%[/tex].

Hope this helps.

Answer: 85.03%

Explanation:

Percentage yield = (actual yield/ theoretical yield) x100

so: (12.33/ 14.50)x 100

= 85.0344827586

Plz help mee
Sandy soil
A) holds water well
B) contains clay and humus
C) has loose grains and few nutrients

Answers

A

think of this way: in Florida and many other costal places, people have sand in their backyards to limit flooding and things due to being so close to the ocean.

After mixing the solutions in a separatory funnel, the stopper should be ________and the liquid should be ________and the layers allowed to separate. When you get close to the interface between the layers, ________the funnel and________until the first layer______ is collected. _______to collect the second layer.

Answers

Answer:

The answer is below

Explanation:

The separation technique is used for separating immiscible liquids.

When separating, the stopper has to be removed when draining the lower layer so as to prevent a vacuum. If vacuum is allowed, the draining rate will reduce and stop.

The liquid should be mixed by shaking the funnel and then opening the stopcock so as the vent out gases.

When near interface between the layers, you should set your eye level so that you do not drain up to the second layer.

After completely draining the first layer, the second layer should be collected in a new flask.

After mixing the solutions in a separatory funnel, the stopper should be removed and the liquid should be mixed thoroughly and the layers allowed to separate. When you get close to the interface between the layers, get eye level with the funnel and slow the draining until the first layer is collected. Switch to a new flask to collect the second layer.

After mixing the solutions in a separatory funnel, the stopper should be removed and the liquid should be mixed thoroughly and the layers allowed to separate. When you get close to the interface between the layers, get eye level with the funnel and slow the draining until the first layer is collected. Switch to a new flask to collect the second layer.

Separatory funnel:It is used in liquid-liquid extractions to separate (partition) the components of a mixture into two immiscible solvent phases of different densities.The lab apparatus majorly used for the separation of the two immiscible solutes in the given mixture. For example, oil and alcohol. It is applicable to separate the solvent which are immiscible and which cannot be separated by steam distillation. This method is useful for liquids only.

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Hello I'm really in a rush this is due tonight, please help I'd really appreciate it if one of you guys answer this, (I hope you guys can help me)

Answers

Answer:

caloricenergylivingmatterkinetic, particleheat, joules

hope this helps :)

What is the specific heat of a 44 g piece of metal if 202 Joules of heat are required to
raise the temperature from 22ᵒC to 33.6ᵒC?

Answers

Answer:

≈ 395,8 J/(kg * °C)

Explanation:

m = 44 g = 0,044 kg

[tex]t_{1}[/tex] = 22 °C

[tex]t_{2}[/tex] = 33,6 °C

Q = 202 J

The formula is: Q = c * m * ([tex]t_{2} - t_{1}[/tex])

c = [tex]\frac{Q}{m * (t_{2} - t_{1} )}[/tex]

Calculating:

c = 202 J / 0,044 kg * (33,6 °C - 22 °C) ≈ 395,8 J/(kg * °C)

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